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Principles of IVF Laboratory Practice

Principles of IVF Laboratory Practice
Optimizing Performance and Outcomes

$69.99 (G)

Alex Lagunov, Michael Crowe, Jason E. Swain, Stephen Troup, Karen Schnauffer, Kara A. Ehlers, Greg L. Christensen, Alison Campbell, Caroline Drew, Kathryn Berrisfor, María José de los Santos, Nicolás Prados, Cristina Hickman, Mina Alikani, Oriol Oliana, Cristina F. L. Hickman, Jonathan Lo, Dawn A. Kelk, Andrew Dorfmann, Dean E. Morbeck, Marius Meintjes, Georgios Liperis, Cecelia Sjöblom, Klaus E. Wiemer, Verena Nordhoff, Sabine Kliesch, Amy E. T. Sparks, Nina Desai, Pooja Rambhia, Marcela Calonge, Satoshi Mizuno, Aisaku Fukuda, Basak Balaban, Laura Rienzi, B. Iussig, F. M. Ubaldi, Caroline McCaffrey, Melicia Clarke-Williams, David H. McCulloh, Dean E. Morbeck, Gianpiero D. Palermo, Tyler Cozzubbo, Queenie V. Neri, Sabine Vanderzwalmen, David Jareno, Pierre Vanderzwalmen, Romain Imber, Maximilian Murtinger, Barbara Wirleitner, Markus H. M. Montag, Thomas Ebner, Dara Berger, Heather S. Hoff, Shunping Wang, Tien-cheng Arthur Chang, Michael Reed, Jun Tao, Courtney Failor, Mitchel C. Schiewe, Anick De Vos, Sangita Jindal, Patty Ann Labella, Rebecca Holmes, C. Brent Barrett, Kathryn J. Go, Thomas B. Pool
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  • Date Published: June 2017
  • availability: In stock
  • format: Paperback
  • isbn: 9781316603512

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  • Since the first successful human in-vitro fertilization (IVF) treatment in 1978, IVF has transformed from an experimental procedure to an established standard of care that is practised throughout the world. As the proven methodologies become more established, patients and practitioners expect higher live birth rates and excellent quality controls. This practical book covers every stage in the life of an IVF laboratory, from designing and planning the laboratory to training the embryologists and ongoing management and troubleshooting, providing step-by-step protocols for today's best practice. Complex and specialized procedures are described by experts in the respective fields using a common structure, which allows the reader to translate into standard operating procedures. The editors bring together a portfolio of techniques that will work in almost every laboratory, providing a foundation of good working practice for both new and experienced embryologists.

    • An easy-to-read, practical book, which avoids the deep theory and instead equips both new and experienced embryologists with the methods needed to provide high-quality, successful IVF
    • Useful from the first day of planning a new IVF laboratory through to quality control and troubleshooting in daily practice
    • Includes very detailed step-by-step instructions for both standard and complex procedures, presented in a style which will be familiar to readers accustomed to following laboratory protocols
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    Product details

    • Date Published: June 2017
    • format: Paperback
    • isbn: 9781316603512
    • length: 308 pages
    • dimensions: 235 x 158 x 13 mm
    • weight: 0.56kg
    • contains: 31 b/w illus. 51 colour illus. 24 tables
    • availability: In stock
  • Table of Contents

    Part I. Starting a New Laboratory and Training Protocols:
    1. Establishing and equipping a new IVF laboratory Alex Lagunov, Michael Crowe and Jason E. Swain
    2. Basic embryology skills in the IVF laboratory Stephen Troup and Karen Schnauffer
    3. Sperm preparation for IVF: training protocol Kara A. Ehlers and Greg L. Christensen
    4. Oocyte pick-up for IVF: training protocol Alison Campbell, Caroline Drew and Kathryn Berrisfor
    5. Embryo transfer for IVF: training protocol María José de los Santos and Nicolás Prados
    6. ICSI for IVF: training protocol Cristina Hickman
    7. Embryo assisted hatching for IVF: training protocol and method Mina Alikani
    8. Embryo biopsy for IVF: training protocol Oriol Oliana, Cristina F. L. Hickman, Jonathan Lo and Dawn A. Kelk
    9. Vitrification for IVF: training protocol Andrew Dorfmann
    10. Mouse embryo assay for quality control in the IVF laboratory Dean E. Morbeck
    11. Sperm survival assay for quality control in the IVF laboratory Marius Meintjes
    Part II. Pre-Procedure Protocols:
    12. Quality control in the IVF laboratory: continuous improvement Georgios Liperis and Cecelia Sjöblom
    13. Set-up procedures for optimizing performance in the IVF laboratory Klaus E. Wiemer
    Part III. Gametes:
    14. Sperm preparation for therapeutic in-vitro fertilization Verena Nordhoff and Sabine Kliesch
    15. Processing surgically retrieved sperm in the IVF laboratory Amy E. T. Sparks
    16. Cryopreservation of sperm for IVF: semen samples and individual sperm Nina Desai and Pooja Rambhia
    17. Oocyte collection for IVF Marcela Calonge
    18. In-vitro maturation of oocytes for IVF Satoshi Mizuno and Aisaku Fukuda
    19. Oocyte grading for IVF Basak Balaban
    20. Vitrification of oocytes for IVF Laura Rienzi, B. Iussig and F. M. Ubaldi
    Part IV. Insemination/ICSI:
    21. Conventional IVF with overnight co-incubation Caroline McCaffrey, Melicia Clarke-Williams and David H. McCulloh
    22. Conventional IVF with short co-incubation Dean E. Morbeck
    23. Technical aspects of ICSI for ejaculated spermatozoa Gianpiero D. Palermo, Tyler Cozzubbo and Queenie V. Neri
    24. Sperm selection for ICSI by morphology Sabine Vanderzwalmen, David Jareno, Pierre Vanderzwalmen, Romain Imber, Maximilian Murtinger and Barbara Wirleitner
    25. Sperm selection for ICSI by viability Markus H. M. Montag
    26. Oocyte activation for IVF Thomas Ebner
    Part V. Fertilization Assessment:
    27. Fertilization assessment in IVF and ICSI Dara Berger and Heather S. Hoff
    28. Polar body biopsy for IVF Markus H. M. Montag
    29. Zygote cryopreservation for IVF Dean E. Morbeck
    Part VI. Embryo Assessment:
    30. Embryo assessment at the precompaction stage in the IVF laboratory Shunping Wang and Tien-cheng Arthur Chang
    31. Embryo assessment at the postcompaction stage in the IVF laboratory Michael Reed and Jun Tao
    32. Embryo assessment at the blastocyst stage in the IVF laboratory Tien-cheng Arthur Chang, Shunping Wang and Courtney Failor
    33. Embryo culture and assessment via time-lapse microscopy in the IVF laboratory Markus H. M. Montag
    Part VII. Embryo Cryopreservation:
    34. Cryopreservation of precompaction embryos for IVF Cecelia Sjöblom
    35. Cryopreservation of postcompaction embryos for IVF Mitchel C. Schiewe
    Part VIII. Embryo Biopsy:
    36. Embryo biopsy by laser on day 5 in the IVF laboratory Anick De Vos
    Part IX. Embryo Transfer:
    37. Routine embryo transfer in the IVF laboratory Sangita Jindal
    Part X. Quality Management:
    38. Quality management in the IVF laboratory: witnessing David H. McCulloh, Patty Ann Labella and Caroline McCaffrey
    39. Quality management in the IVF laboratory: quality improvement, document and process control, and adverse events Rebecca Holmes and C. Brent Barrett
    40. Troubleshooting in the IVF laboratory Kathryn J. Go and Thomas B. Pool.

  • Editors

    Markus H. M. Montag, ilabcomm GmbH, St Augustin, Germany
    Markus H. M. Montag is the CEO and founder of ilabcomm GmbH, a consulting company in the field of assisted reproduction. He was previously laboratory director in a private IVF unit and was at the Universities of Bonn and Heidelberg for twenty years.

    Dean E. Morbeck, Fertility Associates, New Zealand
    Dean E. Morbeck is the Scientific Director for Fertility Associates, based in Auckland, New Zealand, where he oversees the embryology for six IVF clinics.

    Contributors

    Alex Lagunov, Michael Crowe, Jason E. Swain, Stephen Troup, Karen Schnauffer, Kara A. Ehlers, Greg L. Christensen, Alison Campbell, Caroline Drew, Kathryn Berrisfor, María José de los Santos, Nicolás Prados, Cristina Hickman, Mina Alikani, Oriol Oliana, Cristina F. L. Hickman, Jonathan Lo, Dawn A. Kelk, Andrew Dorfmann, Dean E. Morbeck, Marius Meintjes, Georgios Liperis, Cecelia Sjöblom, Klaus E. Wiemer, Verena Nordhoff, Sabine Kliesch, Amy E. T. Sparks, Nina Desai, Pooja Rambhia, Marcela Calonge, Satoshi Mizuno, Aisaku Fukuda, Basak Balaban, Laura Rienzi, B. Iussig, F. M. Ubaldi, Caroline McCaffrey, Melicia Clarke-Williams, David H. McCulloh, Dean E. Morbeck, Gianpiero D. Palermo, Tyler Cozzubbo, Queenie V. Neri, Sabine Vanderzwalmen, David Jareno, Pierre Vanderzwalmen, Romain Imber, Maximilian Murtinger, Barbara Wirleitner, Markus H. M. Montag, Thomas Ebner, Dara Berger, Heather S. Hoff, Shunping Wang, Tien-cheng Arthur Chang, Michael Reed, Jun Tao, Courtney Failor, Mitchel C. Schiewe, Anick De Vos, Sangita Jindal, Patty Ann Labella, Rebecca Holmes, C. Brent Barrett, Kathryn J. Go, Thomas B. Pool

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