Hostname: page-component-848d4c4894-4hhp2 Total loading time: 0 Render date: 2024-05-29T10:50:36.778Z Has data issue: false hasContentIssue false
  • English
  • Français

Alterations of the fatty acid composition and lipid metabolome of breast muscle in chickens exposed to dietary mixed edible oils

Published online by Cambridge University Press:  09 January 2020

X. Y. Cui Open the ORCID record for X. Y. Cui [Opens in a new window] ,
Z. Y. Gou ,
K. F. M. Abouelezz ,
L. Li ,
X. J. Lin ,
Q. L. Fan ,
Y. B. Wang ,
Z. G. Cheng ,
F. Y. Ding  and
S. Q. Jiang
X. Y. Cui
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
Z. Y. Gou
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
K. F. M. Abouelezz
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China Department of Poultry Production, Faculty of Agriculture, Assiut University, Assiut71526, Egypt
L. Li
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
X. J. Lin
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
Q. L. Fan
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
Y. B. Wang
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
Z. G. Cheng
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
F. Y. Ding
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
S. Q. Jiang*
Affiliation:
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou510640, China
*
†E-mail: jsqun3100@sohu.com
Get access

Abstract

The fatty acid composition of chicken’s meat is largely influenced by dietary lipids, which are often used as supplements to increase dietary caloric density. The underlying key metabolites and pathways influenced by dietary oils remain poorly known in chickens. The objective of this study was to explore the underlying metabolic mechanisms of how diets supplemented with mixed or a single oil with distinct fatty acid composition influence the fatty acid profile in breast muscle of Qingyuan chickens. Birds were fed a corn-soybean meal diet supplemented with either soybean oil (control, CON) or equal amounts of mixed edible oils (MEO; soybean oil : lard : fish oil : coconut oil = 1 : 1 : 0.5 : 0.5) from 1 to 120 days of age. Growth performance and fatty acid composition of muscle lipids were analysed. LC-MS was applied to investigate the effects of CON v. MEO diets on lipid-related metabolites in the muscle of chickens at day 120. Compared with the CON diet, chickens fed the MEO diet had a lower feed conversion ratio (P < 0.05), higher proportions of lauric acid (C12:0), myristic acid (C14:0), palmitoleic acid (C16:1n-7), oleic acid (C18:1n-9), EPA (C20:5n-3) and DHA (C22:6n-3), and a lower linoleic acid (C18:2n-6) content in breast muscle (P < 0.05). Muscle metabolome profiling showed that the most differentially abundant metabolites are phospholipids, including phosphatidylcholines (PC) and phosphatidylethanolamines (PE), which enriched the glycerophospholipid metabolism (P < 0.05). These key differentially abundant metabolites – PC (14:0/20:4), PC (18:1/14:1), PC (18:0/14:1), PC (18:0/18:4), PC (20:0/18:4), PE (22:0/P-16:0), PE (24:0/20:5), PE (22:2/P-18:1), PE (24:0/18:4) – were closely associated with the contents of C12:0, C14:0, DHA and C18:2n-6 in muscle lipids (P < 0.05). The content of glutathione metabolite was higher with MEO than CON diet (P < 0.05). Based on these results, it can be concluded that the diet supplemented with MEO reduced the feed conversion ratio, enriched the content of n-3 fatty acids and modified the related metabolites (including PC, PE and glutathione) in breast muscle of chickens.

Keywords

Qingyuan chickenscoconut oilgrowth performancen-3 fatty acidsphospholipids
Type
Research Article
Information
animal , Volume 14 , Issue 6 , June 2020 , pp. 1322 - 1332
Copyright
© The Animal Consortium 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors contributed equally to this work.

References

Alparslan, G and Özdogan, M 2006. The effects of diet containing fish oil on some blood parameters and the performance values of broilers and cost efficiency. International Journal of Poultry Science 5, 415–419.Google Scholar
Baira, E, Dagla, I, Siapi, E, Zoumpoulakis, P, Simitzis, P, Goliomytis, M, Deligeorgis, SG, Skaltsounis, AL and Gikas, E 2018. UHPLC-HRMS-based tissue untargeted metabolomics study of naringin and hesperidin after dietary supplementation in chickens. Food Chemistry 269, 276–285.CrossRefGoogle ScholarPubMed
Balogun, KA, Albert, CJ, Ford, DA, Brown, RJ and Cheema, SK 2013. Dietary omega-3 polyunsaturated fatty acids alter the fatty acid composition of hepatic and plasma bioactive lipids in C57BL/6 mice: a lipidomic approach. PLoS ONE 8, e82399.CrossRefGoogle ScholarPubMed
Bernardi, DM, Bertol, TM, Pflanzer, SB, Sgarbieri, VC and Pollonio, MA 2016. ω-3 in meat products: benefits and effects on lipid oxidative stability. Journal of the Science of Food and Agriculture 96, 2620–2634.CrossRefGoogle ScholarPubMed
Bhatnagar, A, Prasanth Kumar, P, Hemavathy, J and Gopala Krishna, A 2009. Fatty acid composition, oxidative stability, and radical scavenging activity of vegetable oil blends with coconut oil. Journal of the American Oil Chemists’ Society 86, 991–999.CrossRefGoogle Scholar
Chandrashekar, P, Lokesh, B and Krishna, AG 2010. Hypolipidemic effect of blends of coconut oil with soybean oil or sunflower oil in experimental rats. Food Chemistry 123, 728–733.CrossRefGoogle Scholar
Dannenberger, D, Nuernberg, G, Nuernberg, K, Will, K, Schauer, N and Schmicke, M 2017. Effects of diets supplemented with n-3 or n-6 PUFA on pig muscle lipid metabolites measured by non-targeted LC–MS lipidomic profiling. Journal of Food Composition and Analysis 56, 47–54.CrossRefGoogle Scholar
Dayrit, FM 2015. The properties of lauric acid and their significance in coconut oil. Journal of the American Oil Chemists’ Society 92, 1–15.CrossRefGoogle Scholar
Ecker, J, Liebisch, G, Scherer, M and Schmitz, G 2010. Differential effects of conjugated linoleic acid isomers on macrophage glycerophospholipid metabolism. Journal of Lipid Research 51, 2686–2694.CrossRefGoogle ScholarPubMed
Famurewa, AC, Aja, PM, Maduagwuna, EK, Ekeleme-Egedigwe, CA, Ufebe, OG and Azubuike-Osu, SO 2017. Antioxidant and anti-inflammatory effects of virgin coconut oil supplementation abrogate acute chemotherapy oxidative nephrotoxicity induced by anticancer drug methotrexate in rats. Biomedicine and Pharmacotherapy 96, 905–911.CrossRefGoogle ScholarPubMed
Farhoomand, P and Checaniazer, S 2009. Effects of graded levels of dietary fish oil on the yield and fatty acid composition of breast meat in broiler chickens. Journal of Applied Poultry Research 18, 508–513.CrossRefGoogle Scholar
Folch, J, Lees, M and Stanley, GHS 1957. A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226, 495–509.Google ScholarPubMed
Gandemer, G 1997. Muscle lipid and meat quality: phospholipids and flavor. Oleagineux Corps Gras Lipides 4, 19–25.Google Scholar
Harihara Iyer, MN, Sarmah, BC, Tamuli, MK, Das, A and Kalita, D 2012. Effect of dietary sunflower oil and coconut oil on adipose tissue gene expression, fatty acid composition and serum lipid profile of grower pigs. Archives of Animal Nutrition 66, 271–282.CrossRefGoogle Scholar
Huber, M, van de Vijver, LP, Parmentier, H, Savelkoul, H, Coulier, L, Wopereis, S, Verheij, E, van der Greef, J, Nierop, D and Hoogenboom, RA 2010. Effects of organically and conventionally produced feed on biomarkers of health in a chicken model. British Journal of Nutrition 103, 663–676.CrossRefGoogle Scholar
Ian, G 2009. Animal nutrition and lipids in animal products and their contribution to human intake and health. Nutrients 1, 71–82.Google Scholar
Ichihara, K and Fukubayashi, Y 2010. Preparation of fatty acid methyl esters for gas-liquid chromatography. Journal of Lipid Research 51, 635–640.CrossRefGoogle ScholarPubMed
Ji, B, Ernest, B, Gooding, JR, Das, S, Saxton, AM, Simon, J, Dupont, J, Métayer-Coustard, S, Campagna, SR and Voy, BH 2012. Transcriptomic and metabolomic profiling of chicken adipose tissue in response to insulin neutralization and fasting. BMC Genomics 13, 441.CrossRefGoogle ScholarPubMed
Konieczka, P, Czauderna, M and Smulikowska, S 2017. The enrichment of chicken meat with omega-3 fatty acids by dietary fish oil or its mixture with rapeseed or flaxseed-Effect of feeding duration: dietary fish oil, flaxseed, and rapeseed and n-3 enriched broiler meat. Animal Feed Science and Technology 223, 42–52.CrossRefGoogle Scholar
López-Ferrer, S, Baucells, MD, Barroeta, AC and Grashorn, MA 2001. n-3 Enrichment of chicken meat. 1. Use of very long-chain fatty acids in chicken diets and their influence on meat quality: fish oil. Poultry Science 80, 741–752.CrossRefGoogle ScholarPubMed
Miller, D, Johnston, Z, Mullan, B, Pluske, J and Hansen, C 2009. Nutritional manipulation of the somatotropic axis in grower and finisher pigs. Report prepared for the Co-operative Research Centre for an Internationally Competitive Pork Industry, 7–8 December 2009, Australia, pp. 1–12.Google Scholar
Newman, RE, Bryden, WL, Fleck, E, Ashes, JR, Storlien, LH and Downing, JA 2002. Dietary n-3 and n-6 fatty acids alter avian metabolism: molecular-species composition of breast-muscle phospholipids. British Journal of Nutrition 88, 19–28.CrossRefGoogle ScholarPubMed
Panda, A, Sridhar, K, Lavanya, G, Prakash, B, Rao, S and Raju, M 2016. Effect of dietary incorporation of fish oil on performance carcass characteristics meat fatty acid profile and sensory attributes of meat in broiler chickens. Animal Nutrition and Feed Technology 16, 417–425.CrossRefGoogle Scholar
Rahim, NS, Lim, SM, Mani, V, Majeed, ABA and Ramasamy, K 2017. Enhanced memory in Wistar rats by virgin coconut oil is associated with increased antioxidative, cholinergic activities and reduced oxidative stress. Pharmaceutical Biology 55, 825–832.CrossRefGoogle ScholarPubMed
Rymer, C, Gibbs, R and Givens, D 2010. Comparison of algal and fish sources on the oxidative stability of poultry meat and its enrichment with omega-3 polyunsaturated fatty acids. Poultry Science 89, 150–159.CrossRefGoogle ScholarPubMed
Scheeder, MRL, Gläser, KR, Eichenberger, B and Wenk, C 2015. Influence of different fats in pig feed on fatty acid composition of phospholipids and physical meat quality characteristics. European Journal of Lipid Science & Technology 102, 391–401.3.0.CO;2-1>CrossRefGoogle Scholar
Srivastava, Y, Semwal, AD and Swamy, MSL 2013. Hypocholesterimic effects of cold and hot extracted virgin coconut oil (VCO) in comparison to commercial coconut oil: evidence from a male wistar albino rat model. Food Science & Biotechnology 22, 1501–1508.CrossRefGoogle Scholar
Swanson, D, Block, R and Mousa, SA 2012. Omega-3 fatty acids EPA and DHA: health benefits throughout life. Advances in Nutrition 3, 1–7.CrossRefGoogle ScholarPubMed
Wang, J, Wang, X, Li, J, Chen, Y, Yang, W and Zhang, L 2015. Effects of dietary coconut oil as a medium-chain fatty acid source on performance, carcass composition and serum lipids in male broilers. Asian-Australasian Journal of Animal Sciences 28, 223–230.CrossRefGoogle ScholarPubMed
Yeap, SK, Beh, BK, Ali, NM, Yusof, HM, Ho, WY, Koh, SP, Alitheen, NB and Long, K 2015. Antistress and antioxidant effects of virgin coconut oil in vivo. Experimental and Therapeutic Medicine 9, 39–42.CrossRefGoogle ScholarPubMed
Zakaria, Z, Somchit, M, Jais, AM, Teh, LK, Salleh, MZ and Long, K 2011. In vivo antinociceptive and anti-inflammatory activities of dried and fermented processed virgin coconut oil. Medical Principles and Practice 20, 231–236.CrossRefGoogle ScholarPubMed
Zhang, M, Li, D, Li, F, Sun, J, Jiang, R, Li, Z, Han, R, Li, G, Liu, X and Kang, X 2018. Integrated analysis of MiRNA and genes associated with meat quality reveals that Gga-MiR-140-5p affects intramuscular fat deposition in chickens. Cellular Physiology and Biochemistry 46, 2421–2433.CrossRefGoogle ScholarPubMed
Supplementary material: File

Cui et al. supplementary material

Table S1

Download Cui et al. supplementary material(File)
File 94.1 KB
Supplementary material: File

Cui et al. supplementary material

Table S2

Download Cui et al. supplementary material(File)
File 57.2 KB
Supplementary material: File

Cui et al. supplementary material

Table S3

Download Cui et al. supplementary material(File)
File 102.3 KB