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Conjugated linoleic acid and betaine affect lipolysis in pig adipose tissue explants

Published online by Cambridge University Press:  31 May 2019

I. Fernández-Fígares Open the ORCID record for I. Fernández-Fígares [Opens in a new window] ,
M. Lachica Open the ORCID record for M. Lachica [Opens in a new window] ,
M. Martínez-Pérez  and
T. G. Ramsay
I. Fernández-Fígares*
Affiliation:
Departmento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, Granada 18008, Spain
M. Lachica
Affiliation:
Departmento de Fisiología y Bioquímica de la Nutrición Animal, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, Granada 18008, Spain
M. Martínez-Pérez
Affiliation:
Instituto de Ciencia Animal, Carretera Central San José de las Lajas, Mayabeque, Cuba
T. G. Ramsay
Affiliation:
Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, USDA-ARS, 10300 Baltimore Avenue, BARC-East, Beltsville MD 20705, USA
*
†E-mail: ifigares@eez.csic.es
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Abstract

Consumers’ demand of leaner meat products is a challenge. Although betaine and conjugated linoleic acid (CLA) have the potential to decrease porcine adipose tissue, their mode of action is poorly understood. The aim of the study was to determine the lipolytic effect of betaine and CLA in the adipose tissue of Iberian pigs. Adipose tissue explants from five pigs (38 kg BW) were prepared from dorsal subcutaneous adipose tissue samples and cultivated for 2 h (acute experiments) or 72 h (chronic experiments). Treatments included 100 µM linoleic acid (control), 100 µM trans-10, cis-12 CLA, 100 µM linoleic acid + 1 mM betaine and 100 µM trans-10, cis-12 CLA + 1 mM betaine (CLABET). To examine the ability of betaine or CLA to inhibit insulin’s suppression of isoproterenol-stimulated lipolysis, test medium was amended with 1 µM isoproterenol ±10 nM insulin. Media glycerol was measured at the end of the incubations. Acute lipolysis (2 h) was increased by CLA and CLABET (85% to 121%; P < 0.05) under basal conditions. When lipolysis was stimulated with isoproterenol (1090%), acute exposure to betaine tended to increase (13%; P = 0.071), while CLA and CLABET increased (14% to 18%; P < 0.05) isoproterenol-stimulated lipolysis compared with control. When insulin was added to isoproterenol-stimulated explants, lipolytic rate was decreased by 50% (P < 0.001). However, supplementation of betaine to the insulin + isoproterenol-containing medium tended to increase (P = 0.07), while CLABET increased (45%; P < 0.05) lipolysis, partly counteracting insulin inhibition. When culture was extended for 72 h, CLA decreased lipolysis under basal conditions (18%; P < 0.05) with no effect of betaine and CLABET (P > 0.10). When lipolysis was stimulated by isoproterenol (125% increase in rate compared with basal), CLA and CLABET decreased glycerol release (27%; P < 0.001) compared with control (isoproterenol alone). When insulin was added to isoproterenol-stimulated explants, isoproterenol stimulation of lipolysis was completely blunted and neither betaine nor CLA altered the inhibitory effect of insulin on lipolysis. Isoproterenol, and especially isoproterenol + insulin, stimulated leptin secretion compared with basal conditions (68% and 464%, respectively; P < 0.001), with no effect of CLA or betaine (P > 0.10). CLA decreased leptin release (25%; P < 0.001) when insulin was present in the media, partially inhibiting insulin stimulation of leptin release. In conclusion, betaine and CLA produced a biphasic response regarding lipolysis so that glycerol release was increased in acute conditions, while CLA decreased glycerol release and betaine had no effect in chronic conditions. Furthermore, CLA and CLABET indirectly increased lipolysis by reducing insulin-mediated inhibition of lipolysis during acute conditions.

Keywords

fatlipidsmetabolic modifiersmetabolismsus scrofa
Type
Research Article
Information
animal , Volume 13 , Issue 12 , December 2019 , pp. 2840 - 2846
Copyright
© The Animal Consortium 2019 

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