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Effect of mixed meal ingestion on fuel utilization in the whole body and in superficial and deep forearm tissues

Published online by Cambridge University Press:  09 March 2007

Marinos Elia ,
Kurshid Khan  and
Graham Jennings
Marinos Elia*
Affiliation:
Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
Kurshid Khan
Affiliation:
Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
Graham Jennings
Affiliation:
Dunn Clinical Nutrition Centre, Hills Road, Cambridge CB2 2DH, UK
*
*Corresponding author: Dr Marinos Elia, fax +44 (0)1223 413763, email Marinos.Elia@mrc-dunn.cam.ac.uk
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Abstract

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Six healthy lean male adults, given a mixed meal containing 3190 kJ (16% from protein, 44% from carbohydrate and 40% from fat) were studied for the next 370 min using arteriovenous cannulation techniques across superficial and deep forearm tissues. The meal produced no significant change in forearm blood flow or skin temperature. The major differences between superficial and deep forearm tissues were (a) creatinine release by deep tissues but not superficial tissues; (b) the release of non-esterified fatty acids (NEFA) by superficial tissues and uptake by deep tissues; and (c) the more prolonged large positive arteriovenous concentration difference for glucose across deep than superficial tissues. The similarities were (a) general pattern of individual amino acid exchanges and transient positive amino acid N balance after meal ingestion; (b) consistent uptake of glutamate and release of glutamine (the main carrier of N out of superficial and deep forearm tissues); (c) the magnitude of the arteriovenous concentration differences for glucose, NEFA and total amino acids were related to the changes in their circulating concentrations and to the oxidation of carbohydrate, fat, and protein in the whole body; and (d) increases in the arterio–deep venous and arterio–superficial venous differences for glucose did not result in increased release of lactate, alanine or pyruvate, implying no increase in the activity of glucose–lactate and glucose–alanine cycles between forearm tissues and the liver. This study suggests that in a number of ways superficial and deep tissues can be regarded, at least qualitatively, as behaving as a ‘single metabolic unit’.

Keywords

Metabolite exchangeMuscleSkinAdipose tissue
Type
Research Article
Information
British Journal of Nutrition , Volume 81 , Issue 5 , May 1999 , pp. 373 - 381
Copyright
Copyright © The Nutrition Society 1999

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