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Energy expenditure and water turnover in hunting dogs in winter conditions

Published online by Cambridge University Press:  12 October 2011

Øystein Ahlstrøm*
Affiliation:
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Ås, Norway
Paula Redman
Affiliation:
School of Biological Sciences, University of Aberdeen, Aberdeen, Scotland, UK
John Speakman
Affiliation:
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK
*
*Corresponding author: Ø. Ahlstrøm, fax +47 64 96 51 01, email oystein.ahlstrom@umb.no
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Abstract

Hunting with dogs in winter conditions is practised in the Nordic countries. The present study aimed at determining daily energy expenditure (DEE) and body water turnover (BWT) by the doubly labelled water technique in eight hunting dogs (body-weight (BW) range 14–27 kg) working 3 h/d for 3 d ( − 6°C) on ground covered with 20–40 cm of loose snow, to provide information on energy and water requirements. The mean distance run during the hunting period was recorded by the global positioning system and averaged 19·4 km/d. DEE increased with increasing BW (P < 0·001) and varied between 7·20 and 16·6 MJ/d (mean 11·0 MJ/d) corresponding to 950–1350 kJ/kg BW0·75 per d (mean 1170 kJ/kg BW0·75 per d). The larger dogs tended to run longer than the smaller dogs and therefore spent more energy per kg BW0·75 but not significantly (P>0·05). DEE values determined were close to the values measured for hunting dogs running for 3 h/d in hot climates, suggesting that climate within the range of the two studies has little impact on energy expenditure per h running activity. Compared with the work of sled dogs per km travelled running on a track, the work performed by the hunting dogs was suggested to be higher when running in a loose snow layer. However, DEE was much lower because sled dogs ran for a longer distance each day. Mean BWT was 217 ml/kg BW0·75 or 19 ml/kJ metabolisable energy.

Type
Full Papers
Copyright
Copyright © The Authors 2011

Trained hunting dogs (gun dogs) display extraordinary working capacity by running several hours daily and covering long distances. The work that gun dogs do is to assist hunters in finding and retrieving different types of game, mainly birds. Bird hunting is particularly popular in Europe and the USA but also common in other parts of the world. It takes place in the autumn and winter period in various terrains and on different ground conditions, from open fields with solid ground to snow-covered mountain areas. Climate can vary from hot and humid to cold and dry with temperatures as low as − 20°C. Endurance is the main quality required in a gun dog, and dog breeders and trainers show large interest in optimising the feeding of their dogs under various environmental conditions. Scientific information on the daily energy expenditure (DEE) and body water turnover (BWT) of dogs engaged in these activities, which is required for the correct supply of energy and water, is limited. The DEE and BWT have been measured in long-distance sled dogs(Reference Hinchcliff, Reinhart and Burr1, Reference Gerth, Redman and Speakman2), and this has some relevance for hunting dogs, but the daily work load in sled dogs is probably higher as they are pulling a weight and have a longer running period each day. In addition, unlike sled dogs, because of the nature of their work, hunting dogs also have periods of low activity mixed with high-intensity running. Moreover, ground conditions are very different between hunting and sled dogs, which may also affect the daily energy requirements.

A pilot study with two hunting dogs has shown that DEE was higher during hunting in winter conditions compared with controlled exercise, such as running on a treadmill or road running in harness, but results were not conclusive because of the low number of observations(Reference Ahlstrøm, Skrede and Speakman3). In the present study, we aimed to measure DEE and BWT by the doubly labelled water (DLW) technique in eight hunting dogs performing under rough winter conditions.

Materials and methods

Dogs and location

The experiment was conducted at the Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Norway. The use of live animals at the department was authorised by the Norwegian Animal Research Authority. The study comprised eight privately owned dogs (two Irish setters, one English setter, two Gordon setters, two Brittany and one English pointer) with an average body weight (BW) of 19·8 (sd 3·8) kg. The dogs were in good condition and healthy throughout the experiment. The body condition score, scale 1 (lean) to 5 (obese), was 2 for all dogs. The dogs were allowed to hunt with their owners for 3 h/d for three consecutive days in a mountain habitat typical for ptarmigan (Lagopus lagopus) (altitude 1100 m) located at Skinnarbu, Telemark, Norway (59°87′N, 8°58′E). The terrain was a mix of small wooded hills and marshland. The maximum altitude difference was 150 m and hill climbing was moderate. The experiment was divided into two periods, one in late January and another in early February. Of the eight dogs, four were tested in each period. Climatic ( − 6°C, light variable breeze, partly snowy weather) and ground conditions (20–40 cm loose snow on top of a harder snow layer) were similar in the two periods. The start and end point of the hunting sessions was the same for every dog to balance up- and downhill running. When not hunting, the dogs were mainly resting and confined to 10 m2 (owner's room) at room temperature (18–20°C).

Recording of running distance

The running distance covered was recorded by the global positioning system (GPS), type GEKO 201 (Garmin International, Inc., Olathe, KS, USA), weighing 85 g with a highest accuracy of 2 m. The GPS receivers were placed in a small pocket on the dog's marker cloth. The pilot study(Reference Ahlstrøm, Skrede and Speakman3) has shown that identical receivers gave high accuracy for measurement of travelled distance of free running dogs. The receivers were checked regularly during the hunting period, and new batteries were provided after 2 h to avoid power shutdown.

Measurement of energy expenditure and body water turnover

A detailed description of the theoretical background and practical procedures for measuring DEE with the DLW technique has been given elsewhere(Reference Speakman4). We used a mix of high-enrichment isotopes containing 33 % of 2H and 68 % of 18O. Before subcutaneous injection of approximately 6 ml (10− 4 g accuracy) of DLW, a plasma sample was collected to determine the background isotope enrichment. After injection, the DLW was allowed to equilibrate for 5 h before collection of the initial sample. The hunting period started approximately 12 h after the initial sampling, and the final samplings were collected approximately 12 h after the 3 d hunting period. The total time period between the two samplings was 96 h. To measure DEE in the hunting period, the isotope concentrations of the initial sample and the final sample were applied. Blood was sampled in 3 ml vacutainers and centrifuged immediately. Then, three 100 μl glass capillaries were filled with plasma and heat-sealed with a gas torch using pending analysis. The isotope analyses (three replicates) were carried out as described by Speakman et al. (Reference Speakman, Perez-Camargo and McCappin5). Dilution spaces, N d for 2H and N o for 18O, with respective elimination curves (k d and k o) for the isotopes were used to estimate CO2 production. The ‘plateau’ approach was applied for the determination of N d and N o. The dry foods consumed by the dogs contained 22–25 % protein, 20–25 % fat and 35–40 % carbohydrates. For these foods, a RQ of 0·85 was found suitable and therefore applied in the final step of the calculation of energy expenditure as described by Schoeller et al. (Reference Schoeller, Raussin and Schutz6). BW was recorded at the two blood samplings, and means were applied to calculate DEE and BWT.

BWT was calculated by the elimination curve for 2H using the formula:

where k is the elimination rate for 2H, C 1 and C 2 are the initial and final 2H enrichment in body water, respectively, and Δt is the time between C 1 and C 2 sampling.

Statistics

The data presented are single values and means and standard deviations applying the PROC MEANS procedure of SAS(7). Pearson's correlation coefficients (PROC CORR) and a simple regression model (PROC REG) were applied to test the relationship between BW and km travelled, and between BW and DEE, respectively. Relationships between BW and BWT data were tested by PROC REG.

Results

The isotope analyses revealed dilution space ratios (N d:N o) between 1·03 and 1·08 and turnover ratios (k o:k d) for 18O and 2H between 1·33 and 1·50, both of which are in the acceptable range for calculating CO2 production. All dogs were injected with approximately the same volume (6 ml) of DLW irrespective of BW. This volume worked well concerning measurements for all dogs, suggesting that the injection volume could have been reduced for smaller dogs without risking too low concentrations of isotopes at the final blood sampling.

The BW reduction after the hunting period was minor, 1·88 (sd 1·1) %, and BW presented in Table 1 are means of the initial and final BW. The mean running distance for the eight dogs ranged from 15·8 to 24·8 km/d (Table 1). There was a slight tendency that the larger dogs ran longer than the smallest. The reason for this was probably that the smaller dogs had more problems with passing through areas of deep snow than the larger dogs. If the snow was deep, the dogs tended to hop instead of galloping, which they did if the top loose snow layer was thinner. The smallest dogs seemed to hop more frequently, because of their shorter legs, which may have slowed them down compared with the larger dogs with longer legs. However, there was no significant correlation between BW and km travelled (r 2 0·63; P < 0·12).

Table 1 Body weights (BW), running distance, daily energy expenditure (DEE), body water (%) and body water turnover (BWT) data in eight hunting dogs after hunting 3 h/d for three consecutive days

(Mean values and standard deviations)

EE, energy expenditure; S, setter; P, pointer.

As expected, DEE increased with increasing BW (Table 1). The relationship between DEE and BW given by the regression equation, DEE (MJ) = 0·65 × BW (kg) − 1·83, was significant (P < 0·001). The higher DEE with increasing BW was partly due to a higher maintenance requirement and partly because the energy need associated with movement increases with body size. It is interesting to note that by running a similar distance, the smallest dog (dog 8) spent about half of the energy compared with the largest (dog 1). However, the average DEE spent per kg BW0 ·75 was rather similar for the two dogs, 1350 kJ metabolisable energy (ME) and 1080 kJ ME, respectively. Overall, the mean DEE was 11 MJ corresponding to 1170 kJ/kg BW0 ·75 (Table 1).

BWT data, given in Table 1, show that the mean BWT was 2015 ml/d and 217 ml/kg BW0·75 corresponding to 0·19 ml/kJ. As expected, the values showed an increase in BWT (ml/d) with increasing BW. The relationship given by the regression equation BWT (ml/d) = 548+74 × BW (kg) was significant (P < 0·01). No such relationship (P>0·05) was revealed between BW and BWT (ml/kg BW0·75) or between BW and BWT (ml/kJ).

Discussion

The running distance covered by each dog was slightly longer than that measured by the GPS, as the GPS only measures horizontal movements. The terrain used for the experiment was not flat, and exact recordings including up- and downhill distance movements would probably have shown 3–4 % longer distance covered than that recorded by the GPS receivers.

The DEE determined in the present study is in line with a previous pilot study(Reference Hinchcliff, Reinhart and Burr1), in which the dogs were exposed to similar climatic and ground conditions in one part of the study. However, our DEE values were slightly higher than the values reported by Davenport et al. (Reference Davenport, Kelley and Altom8) based on ME consumption in English Pointers hunting for 3 h/d (9·6 MJ/d, 1000 kJ/kg BW0·75). In the latter study, environment and climate were very different from the present study, as the hunting area was fields and woodlands during hot and humid conditions in Georgia, USA. The impact of increased energy requirement due to heat stress during hunting and rest was probably lower in the present study than in the study of Davenport et al. (Reference Davenport, Kelley and Altom8), where the dogs were exposed to high ambient temperature and air moisture. Moreover, the distance covered by the dogs was not recorded in the latter study, and it is therefore difficult to compare the workload of running in the two experiments. However, the similarity of DEE values in the two studies, despite differences in environmental and ground conditions, suggests that trained hunting dogs willing to work with the same intensity will spend about the same amount of energy per h of hunting irrespective of climate and terrain.

Reports on DEE measurements in sled dogs have shown considerably higher levels compared with those measured for the dogs in the present study(Reference Hinchcliff, Reinhart and Burr1, Reference Gerth, Redman and Speakman2). This is mainly because running time per day has been longer for the sled dogs. Hinchcliff et al. (Reference Hinchcliff, Reinhart and Burr1) examined DEE in sled dogs (490 km in 70 h) and found that they spent an impressive 44·6 MJ corresponding to 4400 kJ/kg BW0·75. In another study(Reference Gerth, Redman and Speakman2) with Inuit sled dogs running 60–80 km/d on sea ice, DEE was measured to be 25·7 MJ or 2150 kJ/kg BW0·75. The maintenance energy requirement in the first study was determined to be 550 kJ/kg BW0·75 per d, corresponding to 3850 kJ/kg BW0·75 for muscle work. The dogs ran 168 km/d, which represented an energy cost of 22·9 kJ/kg BW0·75 per km. If a similar maintenance energy requirement is applied for the dogs in the present study, the total DEE would amount to 2·1 times the maintenance energy requirement and the energy spent per km travelled would be 32·0 kJ/kg BW0·75. The dogs in the present study ran at 6·5 km/h, while the sled dogs ran 7 km/h. Even though this type of comparisons should be interpreted with some caution since maintenance energy requirements were not determined in the present study, it may be suggested that running in loose snow as our dogs did is more energy consuming per km than running at the same speed pulling a sledge on a track.

Water supply comes from drinking-water, water in food and water produced from oxidation of the main nutrients (metabolic water), and water losses are represented by water in urine, faeces and evaporated water in respired air. Factors that influence water intake are food intake, dietary salt, ambient temperature and exercise level. Water requirement normally increases parallel to energy expenditure, and a rule of thumb is that the water requirement is 0·24 ml/kJ ME(9). The low BWT indicates that the dogs were little affected by heat stress during hunting, and that the water requirement was lower than that in inactive dogs when measured in relation to energy expenditure.

Conclusions

The present study revealed that DEE in hunting dogs was 1170 kJ/kg BW0·75 when working for 3 h/d in winter conditions ( − 6°C) with a 20–40 cm loose snow layer. BWT was 217 ml/kg BW0·75 or 19 ml/kJ ME.

Acknowledgements

We would like to thank Telemark Fuglehundklubb for allowing us to use their hunting grounds. We acknowledge the dog owners taking part in the study: Siri Kulberg Sjurseth, Finn Holm Nygaard, Per Holm Nygaard, Roy Andersen, Lars Arnesen, Nina Hagesæther, Even Bergseng and Bente Wejden. The study was funded by the Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences. There are no conflicts of interest. The contribution of each author was as follows: Ø. A. conducted the study and wrote the manuscript. P. R. and J. S. carried out the analyses and calculations concerning DLW.

References

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Figure 0

Table 1 Body weights (BW), running distance, daily energy expenditure (DEE), body water (%) and body water turnover (BWT) data in eight hunting dogs after hunting 3 h/d for three consecutive days(Mean values and standard deviations)