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Zinc supplementation has no effect on circulating levels of peripheral blood leucocytes and lymphocyte subsets in healthy adult men

Published online by Cambridge University Press:  09 March 2007

Maxine Bonham ,
Jacqueline M. O'Connor ,
H. Denis Alexander ,
James Coulter ,
Paula M. Walsh ,
Liadhan B. McAnena ,
C. Stephen Downes ,
Bernadette M. Hannigan  and
J. J. Strain
Maxine Bonham*
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
Jacqueline M. O'Connor
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
H. Denis Alexander
Affiliation:
Department of Haematology, Level C, Belfast City Hospital, Belfast BT97AD, UK
James Coulter
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
Paula M. Walsh
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
Liadhan B. McAnena
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
C. Stephen Downes
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
Bernadette M. Hannigan
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
J. J. Strain
Affiliation:
Northern Ireland Centre for Food and Health (NICHE), Department of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK
*
*Corresponding Author: Dr M. Bonham, fax +44 2870 324965, email mp.bonham@ulst.ac.uk
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Abstract

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As a result of evidence documenting harmful effects of Zn supplementation on immune function and Cu status, thirty-eight men were recruited onto a Zn supplementation trial. The aim was to examine the effects of chronic Zn supplementation on circulating levels of peripheral blood leucocytes and lymphocyte subsets. Subjects (n 19) took 30 mg Zn/d for 14 weeks followed by 3 mg Cu/d for 8 weeks to counteract adverse effects, if any, of Zn supplementation on immune status resulting from lowered Cu status. A control group (n 19) took placebo supplements for the duration of the trial. Dietary intakes of Zn approximated 10 mg/d. Blood samples, taken throughout the trial, were assessed for full blood profiles and flow cytometric analyses of lymphocyte subsets. Putative indices of Cu status were also examined. Results indicate that there was no effect of Zn supplementation on circulating levels of peripheral blood leucocytes or on lymphocyte subsets. Cu status was also unaltered. Independent of supplement, there appeared to be seasonal variations in selected lymphocyte subsets in both placebo and supplemented groups. Alterations in circulating levels of B cells (cluster of differentiation (CD) 19), memory T cells (CD45RO) and expression of the intracellular adhesion molecule-1 (CD54) on T cells were observed. Findings indicated no adverse effects of Zn supplementation on immune status or Cu status and support the US upper level of Zn tolerance of 40 mg/d. The seasonal variations observed in lymphocyte subsets in the group as a whole could have implications for seasonal variability in the incidence of infectious diseases.

Keywords

ZincLeucocytesLymphocyte subsetsImmune status
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 5 , May 2003 , pp. 695 - 703
Copyright
Copyright © The Nutrition Society 2003

References

Abo, T & Kumagai, K (1978) Studies of surface immunoglobulin on human B lymphocytes III. Physiological variations of sIg cells in peripheral blood. Clinical and Experimental Immunology 33 441–452.Google Scholar
Allen, KGD & Klevay, LK (1994) Copper: an antioxidant nutrient for cardiovascular health [review]. Current Opinion in Lipidology 5 22–28.Google Scholar
Bala, S, Failla, ML & Lunney, JK (1991) Alterations in splenic lymphoid cell subsets and activation antigens in copper-deficient rats. Journal of Nutrition 121 745–753.CrossRefGoogle ScholarPubMed
Black, MR, Medeiros, DM, Brunett, E & Welke, R (1988) Zinc supplements and serum lipids in young adult white males. American Journal of Clinical Nutrition 47 970–975.Google Scholar
Boukaïba, N, Flament, C, Acher, A, Chappuis, P, Piau, A, Fusselier, M, Dardenne, M & Lemonnier, D (1993) A physiological amount of zinc supplementation: effects on nutritional, lipid and thymic status in an elderly population. American Journal of Clinical Nutrition 57 566–572.Google Scholar
Brown, KH, Peerson, JM & Allen, LH (1998) Effect of zinc supplementation on children's growth: a meta-analysis of intervention trials. Bibliotheca Nutritio et Dieta 54 76–83.Google Scholar
Brown, MA, Thom, JV & Orth, GL (1964) Food poisoning involving zinc contamination. Archives of Environmental Health 8 657–660.Google Scholar
Calvin, J & Price, CP (1986) Measurement of alpha 1 antichymotrypsin by immunoturbidity. Annals of Clinical Biochemistry 23 296–299.CrossRefGoogle Scholar
Chandra, RK (1984) Excessive intake of zinc impairs immune responses. Journal of the American Medical Association 252 1443–1446.CrossRefGoogle ScholarPubMed
Crawley, H (1992) Food Portion Sizes, 3rd ed., London: HM Stationery Office.Google Scholar
Department of Health (1991) Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. London: HM Stationery Office.Google Scholar
Duchateau, J, Delepesse, G, Vrijens, R & Collet, H (1981) Beneficial effects of oral zinc supplementation on the immune responses of old people. American Journal of Medicine 70 1001–1004.CrossRefGoogle ScholarPubMed
Fischer, PWF, Giroux, A & L'Abbe, MR (1984) Effect of zinc supplementation on copper status in adult men. American Journal of Clinical Nutrition 40 743–746.Google Scholar
Fosmire, GJ (1990) Zinc toxicity. American Journal of Clinical Nutrition 51 225–227.Google Scholar
Gidlow, DA, Church, JF & Clayton, BT (1983) Haematological and biochemical parameters in an industrial workforce. Annals of Clinical Biochemistry 20 341–348.Google Scholar
Hambidge, M (2000) Human zinc deficiency. Journal of Nutrition 130 1344s–1349s.Google Scholar
Hambidge, M & Walravens, PA (1982) Disorders of mineral metabolism. Clinical Gastroenterology 11 87–118.Google Scholar
Henry, RJ, Chiamori, N, Jacobs, JL & Segalove, M (1960) Determination of caeruloplasmin oxidase in serum. Proceedings of the Society of Experimental Biology and Medicine 104 620–624.CrossRefGoogle Scholar
Hooper, PL, Visconti, L, Garry, PJ & Johnson, GE (1980) Zinc lowers high density lipoprotein-cholesterol levels. Journal of the American Medical Association 224 1960–1961.CrossRefGoogle Scholar
Institute of Medicine, Food and Nutrition Board (2001) Dietary Reference Intakes: Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium and Zinc. Washington, DC: National Academy Press.Google Scholar
Jones, DG & Suttle, NF (1981) Some effects of copper deficiency on leucocyte function in sheep and cattle. Research in Veterinary Science 31 151–156.CrossRefGoogle ScholarPubMed
Kaplan, J, Hess, JW & Prasad, AS (1988) Impairment of immune function in the elderly: association with mild zinc deficiency. In Essential and Toxic Trace Elements in Human Health and Disease, pp. 309–317New York, NY: Alan R. Liss.Google Scholar
Kelley, DS, Daudu, PA, Taylor, PC, Mackey, BE & Turnlund, JR (1995) Effects of low-copper diets on human immune response. American Journal of Clinical Nutrition 62 412–416.Google Scholar
Klevay, LM, Inman, L, Johnson, LK, Lawler, M, Mahalko, JR, Milne, DB, Lukaski, HC, Bolonchuk, W & Sandstead, HH (1984) Increased cholesterol in plasma in a young man during experimental copper depletion. Metabolism 33 1112–1118.CrossRefGoogle Scholar
MacMurray, JP, Barker, JP, Armstrong, JD, Bozzetti, LP & Kuhn, IN (1983) Circannual changes in immune function. Life Sciences 32 2363–2370.CrossRefGoogle ScholarPubMed
Nielsen, FH, Milne, DB, Mullen, LM & Gallagher, SK (1990) Dietary sulphur amino acids, and genetic make-up or interindividual variation affect the response of men to copper depletion. Journal of Trace Elements in Experimental Medicine 3 281–296.Google Scholar
Prasad, AS, Fitzgerald, JT, Hess, JW, Kaplan, J, Pelen, F & Dardenne, M (1993) Zinc deficiency in elderly patients. Nutrition 9 218–224.Google Scholar
Prasad, AS, Miale, A, Farid, Z, Sandstead, HH & Schulert, AR (1963) Zinc metabolism in normals and patients with the syndrome of iron deficiency anemia, hepatosplenomegaly, dwarfism and hypogonadism. Journal of Laboratory and Clinical Medicine 61 537–548.Google Scholar
Provinciali, M, Montenovo, A, Di Stefano, G, Colombo, M, Daghetta, L, Cairati, M, Veroni, C, Cassino, R, Della Torre, F & Fabris, N (1998) Effect of zinc or zinc plus arginine supplementation on antibody titre and lymphocyte subsets after influenza vaccination in elderly subjects: a randomized controlled trial. Age and Ageing 27 715–722.Google Scholar
Reichert, T, DeBruyère, V, Deneys, V, Tötterman, T, Lydyard, P, Yuksel, F, Chapel, H, Jewell, D, Van Hove, L, Linden, J & Buchner, L (1991) Lymphocyte reference ranges in adult caucasians. Clinical Immunology and Immunopathology 60 190–208.Google Scholar
Reinberg, A, Schuller, E, Clench, J & Smolensky, MH (1980) Recent Advances in the Chronobiology of Allergy and Immunology, p. 251 [Smolensky, MH, editor]. New York, NY: Pergamon Press.Google Scholar
Reinberg, A & Smolensky, M (1983) Biological Rhythms and Medicine. Cellular, Metabolic, Physiopathologic and Pharmacologic Aspects. Berlin and Heidelberg, Germany and New York, NY: Springer.Google Scholar
Rink, L & Kirchner, H (2000) Zinc-altered immune function and cytokine production. Journal of Nutrition 130 1407s–1411s.Google Scholar
Samman, S & Roberts, DCK (1988) The effect of zinc supplements on lipoproteins and copper status. Atherosclerosis 70 247–252.Google Scholar
Sandstead, HH (1995) Is zinc deficiency a public health problem? Nutrition 11 87–92.Google Scholar
Sandstead, HH & Smith, S Jr (1996) Deliberations and evaluations of approaches, endpoints and paradigms for determining zinc dietary recommendations. Journal of Nutrition 126 2410s–2418s.Google Scholar
Schlesinger, L, Arevalo, M, Arredondo, S, Lönnerdal, B & Stekel, A (1993) Zinc supplementation impairs monocyte function. Acta Paediatrica 82 734–738.Google Scholar
Underwood, EJ (1977) Trace Elements in Human and Animal Nutrition. New York, NY: Academic Press.Google Scholar
Walvarens, PA, Hambidge, KM & Koepfer, DM (1989) Zinc supplementation in infants with a nutritional pattern of failure to thrive: a double blind controlled study. Paediatrics 83 532–538.Google Scholar
Wood, RJ (2000) Assessment of marginal zinc status in humans. Journal of Nutrition 130 1350s–1354s.Google Scholar
Yadrick, MK, Kenney, MA & Winterfeldt, EA (1989) Iron, copper, and zinc status: response to supplementation with zinc or zinc and iron in adult females. American Journal of Clinical Nutrition 49 145–150.Google Scholar