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Identification and tissue expression profiling of candidate UDP-glycosyltransferase genes expressed in Holotrichia parallela motschulsky antennae

Published online by Cambridge University Press:  05 February 2018

S. Wang ,
Y. Liu ,
J.-J. Zhou ,
J.-K. Yi ,
Y. Pan ,
J. Wang ,
X.-X. Zhang ,
J.-X. Wang ,
S. Yang  and
J.-H. Xi
S. Wang
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
Y. Liu
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
J.-J. Zhou
Affiliation:
Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK
J.-K. Yi
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
Y. Pan
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
J. Wang
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
X.-X. Zhang
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
J.-X. Wang
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
S. Yang
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
J.-H. Xi*
Affiliation:
College of Plant Science, Jilin University, Changchun 130062, P.R. China
*
*Author for correspondence Phone: +86-13756072796 Fax: +86-431-87836255 E-mail: jhxi1965@jlu.edu.cn
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Abstract

It is difficult to control Holotrichia parallela Motschulsky with chemical insecticides due to the larvae's soil-living habit, thus the pest has caused great economic losses in agriculture. In addition, uridine diphosphate-glycosyltransferases (UGTs) catalyze the glycosylation process of a variety of small lipophilic molecules with sugars to produce water-soluble glycosides, and play multiple roles in detoxification, endobiotic modulation, and sequestration in an insect. Some UGTs were found specifically expressed in antennae of Drosophila melanogaster and Spodoptera littoralis, and glucurono-conjugated odorants could not elicit any olfactory signals, suggesting that the UGTs may play roles in odorant inactivation by biotransformation. In the current study, we performed a genome-wide analysis of the candidate UGT family in the dark black chafer, H. parallela. Based on a UGT gene signature and the similarity of these genes to UGT homologs from other organisms, 20 putative H. parallela UGT genes were identified. Bioinformatics analysis was used to predict sequence and structural features of H. parallela UGT proteins, and revealed important domains and residues involved in sugar donor binding and catalysis by comparison with human UGT2B7. Phylogenetic analysis of these 20 UGT protein sequences revealed eight major groups, including both order-specific and conserved groups, which are common to more than one order. Of these 20 UGT genes, HparUGT1265-1, HparUGT3119, and HparUGT8312 were highly (>100-fold change) expressed in antennae, suggesting a possible role in olfactory tissue, and most likely in odorant inactivation and olfactory processing. The remaining UGT genes were expressed in all tissues (head, thorax, abdomen, leg, and wing), indicating that these UGTs likely have different biological functions. This study provides the fundamental basis for determining the function of UGTs in a highly specialized olfactory organ, the H. parallela antenna.

Keywords

UDP-glycosyltransferaseHolotrichia parallelaantennaeolfaction
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 6 , December 2018 , pp. 807 - 816
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Copyright
Copyright © Cambridge University Press 2018 

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