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Activities and Kinetic Properties of Lumbar Cerebrospinal Fluid Cholinesterases in Relation to Clinical Diagnosis, Severity, and Progression of Alzheimer's Disease

Published online by Cambridge University Press:  18 September 2015

F. Jacob Huff ,
Clara T. Reiter  and
Jack Protetch
F. Jacob Huff*
Affiliation:
Departments of Neurology and Psychiatry, Alzheimer's Disease Research Center, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine
Clara T. Reiter
Affiliation:
Departments of Neurology and Psychiatry, Alzheimer's Disease Research Center, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine
Jack Protetch
Affiliation:
Departments of Neurology and Psychiatry, Alzheimer's Disease Research Center, Western Psychiatric Institute and Clinic, University of Pittsburgh School of Medicine
*
Neuroscience Research Group, Hoechst-Roussel Pharmaceuticals, Routes 202-206, Building M-338, Somerville, N.J., USA 08876
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Abstract:

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Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities of lumbar cerebrospinal fluid (CSF) have been measured in seventeen patients with a clinical diagnosis of probable Alzheimer's disease (Prob AD), possible Alzheimer's disease (Poss AD), or dementia of non-Alzheimer aetiology (Non-AD). The three diagnostic groups did not differ with regard to the Km or saturation kinetic properties of AChE and BChE. The CSF AChE activity was significantly higher in Prob AD than in Non-AD patients. The groups did not differ significantly in BChE activity. The ratio of AChE to BChE activity was significantly higher in both the Prob AD and Poss AD groups than in the Non-AD group, and the ranges of values in the Prob AD and Non-AD groups did not overlap. Among patients in the Prob AD group, severity of dementia was correlated with both AChE activity and the AChE/BChE ratio, and progression of dementia over time was also correlated with AChE/BChE.

The AChE/BChE ratio correlated more strongly than AChE with severity and progression of dementia in Prob AD patients, and also better distinguished them from Non-AD patients, suggesting that AChE/BChE may be the more useful marker for diagnosis of AD. It is not clear from the results whether AChE/BChE is useful for diagnosis of the complex dementia cases in the Poss AD group.

Résumé:

RÉSUMÉ:

L'activité de l'acétyl-cholinestérase (AChE) et de la butyrylcholinestérase (BChE) du liquide céphalo-rachidien (LCR) lombaire ont été mesurées chez dix-sept patients avec un diagnostic de maladie d'Alzheimer probable (Prob MA), maladie d'Alzheimer possible (Poss MA), ou démence d'étiologie autre (Non-MA). Les trois groupes de patients ne différaient pas quant au Km ou auz propriétés cinétiques de saturation de l'AChE et de la BChE. L'activité de l'AChE dans le LCR était significativement plus élevée chez les Prob MA que chez les Non-MA. Les deux groupes ne différaient pas significativement quant à l'activité de la BChE. Le rapport entre l'activité AChE et l'activité BChE était significativement plus élevé dans les groupes Prob MA et Poss MA que dans le groupe Non-MA, et l'écart des valeurs dans les groupes Prob MA et Non-MA ne se chevauchaient pas. Parmi les patients du groupe Prob MA, la sévérité de la démence était en corrélation avec l'activité de l'AChE et le rapport AChE/BChE, et la progression de la démence dans le temps était également en corrélation avec l'activité de l'AChE et le rapport AChE/BChE, et la progression de la démence dans le temps était également en corrélation avec l'AChE/BChE.

La corrélation entre le rapport AChE/BChE et la sévérité et la progression de la démence chez les patients Prob MA était plus forte que celle de l'AChE et les distinguait mieux des patients Non-MA, suggérant que l'AChE/BChE peut être le marqueur le plus utile pour le diagnostic de la MA. Ces résultats ne nous permettent pas de déterminer si l'AChE/BChE est utile dans le diagnostic des cas complexes de deménce du groupe Poss MA.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 16 , Issue 4 , November 1989 , pp. 406 - 410
Copyright
Copyright © Canadian Neurological Sciences Federation 1989

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