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Focal Cerebral Ischemia in Rats: Effect of Hemodilution with α-α Cross-Linked Hemoglobin on Brain Injury and Edema

Published online by Cambridge University Press:  18 September 2015

Daniel J. Cole ,
Randall M. Schell ,
John C. Drummond ,
Robert J. Pryzbelski  and
Suzzane Marcantonio
Daniel J. Cole*
Affiliation:
Departments of Anesthesiology, Loma Linda University, Loma Linda, California (D.J.C., R.M.S., R.J.P., S.M.), VA Medical Center. San Diego, California (J.C.D.), and University of California at San Diego, La Jolla. California (J.C.D.)
Randall M. Schell
Affiliation:
Departments of Anesthesiology, Loma Linda University, Loma Linda, California (D.J.C., R.M.S., R.J.P., S.M.), VA Medical Center. San Diego, California (J.C.D.), and University of California at San Diego, La Jolla. California (J.C.D.)
John C. Drummond
Affiliation:
Departments of Anesthesiology, Loma Linda University, Loma Linda, California (D.J.C., R.M.S., R.J.P., S.M.), VA Medical Center. San Diego, California (J.C.D.), and University of California at San Diego, La Jolla. California (J.C.D.)
Robert J. Pryzbelski
Affiliation:
Departments of Anesthesiology, Loma Linda University, Loma Linda, California (D.J.C., R.M.S., R.J.P., S.M.), VA Medical Center. San Diego, California (J.C.D.), and University of California at San Diego, La Jolla. California (J.C.D.)
Suzzane Marcantonio
Affiliation:
Departments of Anesthesiology, Loma Linda University, Loma Linda, California (D.J.C., R.M.S., R.J.P., S.M.), VA Medical Center. San Diego, California (J.C.D.), and University of California at San Diego, La Jolla. California (J.C.D.)
*
Department of Anesthesiology. Loma Linda University, Loma Linda, California, U.S.A. 92354
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Abstract:

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The effect of hemodilution, with α-α cross-linked hemoglobin (DCLHb), on brain injury and edema was assessed after temporary middle cerebral artery occlusion in rats. Injury was analyzed with 2,3,5-triphenyltetrazolium chloride (TTC) stain and edema by microgravimety. Part A: DCLHb was given to maintain one of the following hematocrits (Hct) and normotension: 1) 45/Hct, 2) 30/Hct, 3) 16/Hct, or 4) 9/Hct. Brain injury (% of ischemic hemisphere, mean ± SD) was less in the 30/Hct group (31 ±4) versus the 45/Hct group (42 ± 5); and in the 16/Hct (20 ± 3) and 9/Hct (19 ± 4) groups versus the 45/Hct and 30/Hct groups. Edema was less in the hemodiluted groups versus the 45/Hct group. Part B: DCLHb was given to maintain one of the following hematocrits and hyper (HTN) − or normotension (Norm): 1) 45/Norm, 2) 30/Norm, 3) 30/HTN, 4) 16/Norm, or 5) 16/HTN. In hematocrit matched groups hypertension decreased brain injury (30/HTN − 24 ± 2 < 30/Norm − 34 ± 4; and 16/HTN − 17 ± 3 < 16/Norm − 24 ± 4). Edema was not effected by hypertension. These results suggest that hemodilution with DCLHb decreases focal ischemic injury, and is most effective when given in a manner that induces hypertension.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 20 , Issue 1 , February 1993 , pp. 30 - 36
Copyright
Copyright © Canadian Neurological Sciences Federation 1993

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