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Development of specific SSR marker for Ee genome of Thinopyrum spp. using wheat microsatellites

Published online by Cambridge University Press:  12 February 2007

You Ming-Shan ,
Li Bao-Yun ,
Tian Zhi-Hui ,
Tang Zhao-Hui ,
Liu Shou-Bin  and
Liu Guang-Tian
You Ming-Shan*
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Li Bao-Yun
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Tian Zhi-Hui
Affiliation:
Department of Horticulture and Forestry, Beijing Agricultural College, Beijing 102206, China
Tang Zhao-Hui
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Liu Shou-Bin
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Liu Guang-Tian
Affiliation:
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
*
*Corresponding author: E-mail: msyou67@cau.edu.cn
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Abstract

Wheatgrass, Thinopyrum Löve (Elytrigia Nevski), is one of the most important alien resources for wheat improvement. To determine the transferability of wheat microsatellite primers to Thinopyrum spp., 40 wheat microsatellite primer pairs were selected to perform PCR analysis on 17 accessions of Thinopyrum spp. and two common wheat (Triticum aestivum) cultivars. Among the 40 primer pairs used, 25 pairs could produce PCR products on all or most of the alien species. Moreover, all of these 25 primer pairs, except Xgwm325, showed polymorphism in the Thinopyrum accessions. In total, 108 alleles were detected by the 25 primer pairs. The number of alleles per primer pair varied from 1 to 8, with an average of 4.3 alleles per primer. Although primer Xgwm325 did not detect polymorphic alleles in Thinopyrum spp., it could be used as a specific SSR marker for them. It amplified four specific DNA fragments in Thinopyrum spp. of length approximately 1400, 440, 120 and 100 bp. To verify the validity of these molecular markers, a further PCR programme with primer Xgwm325 was conducted on a full set of seven wheat–Thinopyrum elongatum disomic addition lines and 15 disomic substitution lines. This showed that only the 100-bp fragment appeared in all materials possessing the alien chromosome, indicating that it was a molecular marker for the entire genome of Th. elongatum, although primer Xgwm325 was specific for chromosome 6D in wheat.

Keywords

ThinopyrumgenomeSSR marker
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 1 , Issue 3 , December 2004 , pp. 143 - 148
Copyright
Copyright © China Agricultural University and Cambridge University Press 2004

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