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Characterization of non-typable strains of Staphylococcus aureus from cases of hospital infection

Published online by Cambridge University Press:  19 October 2009

Ana Vindel ,
Cecilia Martín-Bourgon ,
Juan A. Saez-Nieto  and
Saez Nieto
Ana Vindel
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
Cecilia Martín-Bourgon
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
Juan A. Saez-Nieto
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
Saez Nieto
Affiliation:
Laboratorio de Referenda de Fagolipia de S. aureus, Servicio de Bacteríologia, Centro Nacional de Microbiología, Virología e Immunología Sanitarias, 28220 Majadahonda, Madrid, Spain
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Summary

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A high percentage of non-typable (NT) Staphylococcus aureus strains was isolated in Spanish hospitals during 1984 and 1985. Several alternative methods of typing were employed to study these isolates. These were: phage-typing at 1000 × RTD, phage-typing after heat-treatment (48 °C), thermal shock (56 °C), reverse-typing and induction of additional phages. Using these methods the number of NT isolates was reducedby 60%. Best results were obtained with heat-treatment. Additional phages and reverse-typing were also useful.

A scheme for the study of outbreaks and sporadic cases caused by NT strains is proposed using the methods described.

Type
Research Article
Information
Epidemiology & Infection , Volume 99 , Issue 1 , August 1987 , pp. 191 - 200
Copyright
Copyright © Cambridge University Press 1987

References

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