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The distribution of the DHFR genes in trimethoprim-resistant urinary tract isolates from Taiwan

Published online by Cambridge University Press:  15 May 2009

Lin-Li Chang ,
Shui-Feng Chang ,
Teh-Yuan Chow ,
Wen-Jeng Wu  and
Jong-Chou Chang
Lin-Li Chang
Affiliation:
Department of Microbiology, Taiwan, Republic of China
Shui-Feng Chang*
Affiliation:
Department of Microbiology, Taiwan, Republic of China
Teh-Yuan Chow
Affiliation:
Institute of Botany, Academia Sinica, Taipei
Wen-Jeng Wu
Affiliation:
Department of Urology, Kaohsiung Medical College, Kaohsiung
Jong-Chou Chang
Affiliation:
Department of Obstetrics and Gynecology, Chang Gang Memorial Hospital, Kaohsiung 807, Taiwan, Republic of China
*
*Requests for reprints to: Dr Shui-Feng Chang, Department of Microbiology, Kaohsiung Medical College, Kaohsiung 807, Taiwan, Republic of China.
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Between July 1987 and June 1989, 1054 urinary isolates of enterobacteria from Kaohsiung, Taiwan were studied for their trimethoprim resistance. Trimethoprim resistance was defined as MIC greater than 4 μg/ml and high-level resistance by MIC greater than 1000 μg/ml. The incidence of trimethoprim resistance increased from 33·6% in 1987 to 42·1% in 1989. Among the resistant strains studied, 90% were resistant to high levels of trimethoprim. An increase in the proportion of resistant strains (33·9–46·3%) exhibiting high-level non-transferable trimethoprim resistance was noted. The distribution of the dihydrofolate reductase (DHFR) genes by colony hybridization in 374 trimethoprim-resistant isolates revealed the presence of type I and type V DHFR genes in most of these isolates (45·4% and 10·4% respectively). Type I was predominant in Escherichia coli whereas type V was frequently seen in Enterobacter spp. None showed homology with the type II and type III DHFR probe DNA. In addition, transposon Tn7 was present in 7·8% of 374 trimethoprim-resistant enterobacteria.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 3 , December 1992 , pp. 453 - 462
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Richmond, M. Antibiotic resistance and the evolution of bacteria. Nature 1983; 302: 657.CrossRefGoogle ScholarPubMed
2.Huovinen, P, Pulkkinen, L, Helin, HL, Makila, M, Toivanen, P. Emergence of trimethoprim resistance in relation to drug consumption in a Finnish hospital from 1971 through 1984. Antimicrob Agents Chemother 1986; 29: 73–6.CrossRefGoogle Scholar
3.Amyes, SGB, Smith, JT. R-factor trimethoprim resistance mechanism: An insusceptible target site. Biochem Biophys Res Commun 1974; 58: 412–8.CrossRefGoogle ScholarPubMed
4.Amyes, SGB, Towner, KJ. Trimethoprim resistance; epidemiology and molecular aspects. J Med Microbiol 1990; 31: 1–19.CrossRefGoogle Scholar
5.Jansson, C, Sköld, O. Appearance of a new trimethoprim resistance gene, dhfrIX, in Escherichia coli from Swine. Antimicrob Agents Chemother 1991; 35: 1891–9.CrossRefGoogle ScholarPubMed
6.Steen, R, Sköld, O. Plasmid-borne or chromosomally mediated resistance by Tn7 is the most common response to ubiquitous use of trimethoprim. Antimicrob Agents Chemother 1985; 27: 933–7.Google Scholar
7.Pulkkinen, L, Huovinen, P, Vuorio, E, Toivanen, P. Characterization of trimethoprim resistance by use of probes specific for transposon Tn7. Antimicrob Agents Chemother 1984; 26: 82–6.CrossRefGoogle ScholarPubMed
8.Mayer, KH, Fling, ME, Hopkins, JD, O'Brien, TF. Trimethoprim resistance in multiple genera of Enterobacteriaceae at a U.S. hospital: spread of the type II dihydrofolate reductase gene by a single plasmid. J Infect Dis 1985; 151: 783–9.Google Scholar
9.Fling, ME, Kopf, J, Richards, C. Characterization of plasmid pAZ1 and the type III dihydrofolate reductase gene. Plasmid 1988; 19: 30–3.CrossRefGoogle ScholarPubMed
10.Sundström, L, Vinayagamoorthy, T, Sköld, O. Novel type of plasmid-borne resistance to trimethoprim. Antimicrob Agents Chemother 1987; 31: 60–6.CrossRefGoogle ScholarPubMed
11.Chang, LL, Chang, SF, Chow, TY. R factor in urinary tract infection. J Formosan Med Assoc 1984; 83: 268–77.Google ScholarPubMed
12.Datta, N, Richards, H. Trimethoprim-resistant bacteria in hospital and in the community: spread of plasmids and transposons. In: Levy, SB, Clowes, RC, Koenig, RL, eds. Molecular biology: pathogenicity and ecology of bacterial plasmids. New York: 1981: 21–30.CrossRefGoogle Scholar
13.Chang, SF, Chang, LL, Chow, TY, Wu, WJ, Chang, JC. Prevalence of transposons encoding kanamycin, ampicillin and trimethoprim resistance in isolates from urinary tract infections detected by using DNA probes. Kaohsiung J Med Sci 1992; 8: 141–7.Google ScholarPubMed
14.Lin, SR, Chang, SF. Drug resistance and plasmid profile of shigellae in Taiwan. Epidemiol Infect 1991; 108: 87–97.CrossRefGoogle Scholar
15.Kado, CI, Liu, ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–73.CrossRefGoogle ScholarPubMed
16.Maniatis, T, Fritsch, EF, Sambrook, J. Molecular cloning, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1989.Google Scholar
17.Gosti-Testu, F, Norris, V, Brevet, J. Restriction map of Tn7. Plasmid 1983; 10: 96–9.CrossRefGoogle ScholarPubMed
18.Birnboim, HC, Doly, J.A. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7: 1513–23.CrossRefGoogle ScholarPubMed
19.Goldstein, FW, Papadopoulou, B, Acar, JF. The changing pattern of trimethoprim resistance in Paris, with a review of worldwide experience. Rev Infect Dis 1986; 8: 725–37.Google Scholar
20.Romero, E, Perduca, M. Compatibility groups of R-factors for trimethoprim resistance isolated in Italy. J Antimicrob Chemother 1977; 3: 35–8.CrossRefGoogle ScholarPubMed
21.Huovinen, P, Pulkkinen, L, Toivanen, P. Transferable trimethoprim resistance in three Finnish hospitals. J Antimicrob Chemother 1983; 12: 249–56.CrossRefGoogle ScholarPubMed
22.Brumfitt, W, Hamilton-Miller, JMT, Wood, A. Evidence for a slowing in trimethoprim resistance during 1981 – a comparison with earlier years. J Antimicrob Chemother 1983; 11: 503–9.CrossRefGoogle ScholarPubMed
23.Murray, BE, Alvarado, T, Kim, KH, et al. Increasing resistance to trimethoprim-sulfamethoxazole among isolates of Escherichia coli in developing countries. J Infect Dis 1985; 152: 1107–13.CrossRefGoogle ScholarPubMed
24.Fleming, MP, Datta, N, Grüneberg, RN. Trimethoprim resistance determined by R factors. Br Med J 1972; 1: 726–8.CrossRefGoogle ScholarPubMed
25.Jobanputra, RS, Datta, N. Trimethoprim R factors in enterobacteria from clinical specimens. J Med Microbiol 1974; 7: 169–77.CrossRefGoogle ScholarPubMed
26.Bannatyne, RM, Toma, S, Cheung, R, Hu, G. Resistance to trimethoprim and other antibiotics in Ontario Shigellae. Lancet 1980; i: 425–6.Google Scholar
27.Threlfall, EJ, Rowe, B, Huq, I. Plasmid-encoded multiple antibiotic resistance in Vibrio Cholerae E1 Tor from Bangladesh. Lancet 1980; i: 1247–8.CrossRefGoogle Scholar
28.Kraft, CA, Timbury, MC, Platt, DJ. Distribution and genetic location of Tn7 in trimethoprim-resistant Escherichia coli. J Med Microbiol 1986; 22: 125–31.Google Scholar
29.Heikkila, E, Sundström, L, Skurnik, M, Huovinen, P. Analysis of genetic localization of the type I trimethoprim resistance gene from Escherichia coli isolated in Finland. Antimicrob Agents Chemother 1991; 35: 1562–9.CrossRefGoogle ScholarPubMed
30.Radstrom, P, Swedberg, G, Sköld, O. Genetic analyses of sulfonamide resistance and its dissemination in gram-negative bacteria illustrate new aspects of R plasmid evolution. Antimicrob Agents Chemother 1991; 35: 1840–8.Google Scholar
31.Sundström, L, Radstrom, P, Swedberg, G, Sköld, O. Site specific recombination promotes linkage between trimethoprim and sulfonamide resistance genes. Sequence characterization of dhfrV and sull and a recombination active locus of Tn21. MGG 1988; 213: 191–201.Google Scholar
32.Sundström, L, Sköld, O. The dhfrI trimethoprim resistance gene of Tn7 can be found at specific sites in other genetic surroundings. Antimicrob Agents Chemother 1990; 34: 642–50.Google Scholar