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The relationship between the serogroup antigen and lipopolysaccharide of Legionella pneumophila

Published online by Cambridge University Press:  19 October 2009

J. W. Conlan  and
L. A. E. Ashworth
J. W. Conlan
Affiliation:
Experimental Pathology Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, UK
L. A. E. Ashworth
Affiliation:
Experimental Pathology Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, UK
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Serogroup-specific antigen was extracted from a number of Legionella pneumophila strains and compared with phenol-water extracted lipopolysaccharide on the basis of gel filtration, chemical analysis, SDS-PAGE and reaction with serogroup-specific antibody in immunoblots. Serogroup specificity is apparently borne by the O side-chains of the lipopolysaccharide, which, although smooth in type, partitions in the phenol phase. For four serogroup 1 strains tested, there was no qualitative correlation between O side-chain length and pulmonary virulence for guinea-pigs.

Type
Research Article
Information
Journal of Hygiene , Volume 96 , Issue 1 , February 1986 , pp. 39 - 48
Copyright
Copyright © Cambridge University Press 1986

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