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Rapid Epidemiologic Characterization of Cytomegalovirus Strains From Pediatric Bone Marrow Transplant Patients

Published online by Cambridge University Press:  02 January 2015

Inara E. Souza ,
Donald Nicholson ,
Sandra Matthey ,
Beth Alden ,
Thomas H. Haugen ,
Michael E. Trigg  and
James E Bale Jr
Inara E. Souza*
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa Department of Pediatrics at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Donald Nicholson
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Sandra Matthey
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Beth Alden
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Thomas H. Haugen
Affiliation:
Department of Pathology at the University of Iowa Hospital and Clinics, Iowa City, Iowa
Michael E. Trigg
Affiliation:
Department of Pediatrics at the University of Iowa Hospital and Clinics, Iowa City, Iowa
James E Bale Jr
Affiliation:
Department of Pediatrics at the University of Iowa Hospital and Clinics, Iowa City, Iowa
*
Escola Paulista de Medicine, Sao Paulo, Brazil
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Abstract

Background:

DNA amplification by the polymerase chain reaction (PCR) of human cytomegalovirus (CMV) nucleotide sequences recently has been reported for differentiation of CMV strains.

Design:

Retrospective study.

Objective and Patients:

Evaluate the strain patterns of 15 CMV-positive buffy coat specimens from five pediatric bone marrow transplant patients.

Setting:

Pediatric bone marrow transplant unit.

Methods:

We performed PCR using primers corresponding to two distinct regions of the CMV genome, the major immediate-early (MIE) region and the a-sequence region, with subsequent restriction enzyme analysis of the amplified products.

Results:

Restriction enzyme analysis with Hae III and Hinf I of products amplified with nested PCR for the MIE region revealed distinguishable digestion patterns between patients but similar patterns for samples from each patient. All were distinct from the CMV Towne laboratory control strain. In contrast to these results, amplification of specimens with a-sequence primers, followed by restriction enzyme analysis, did not allow differentiation between all patients.

Conclusion:

Our results indicate that nested amplification directly from buffy coat specimens using primers for the CMV MIE gene allows rapid CMV strain characterization that is useful for laboratory quality control and epidemiological studies. Distinct CMV strains were found in each patient, suggesting horizontal transmission was not responsible for acquisition of CMV infection in these patients.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 16 , Issue 7 , July 1995 , pp. 399 - 404
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1995

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References

REFERENCES

1.Winston, DJ, Huang, ES, Miller, MJ, et al. Molecular epidemiology of cytomegalovirus infections associated with bone marrow transplant. Ann Intern Med 1985;102:16–20.Google Scholar
2.Murph, JR, Bale, JEMurray, JC, Stinski, MEPerlman, S. Cytomegalovirus transmission in a Midwest day care center: possible relationship to child care practices. J Pediatr 1986;109:35–39.Google Scholar
3.Wilfert, CM, Huang, ES, Stagno, S. Restriction endonuclease analysis of cytomegalovirus deoxyribonucleic acid as an epidemiologic tool. Pediattics 1982;70:711–721.Google Scholar
4.Adler, SPThe molecular epidemiology of cytomegalovirus transmission among children attending a day care center. J Infect Dis 1985;152:760–768.Google Scholar
5.Spector, SA, Neuman, TR, Hirata, KK. Rapid determination of molecular relatedness of isolates of human cytomegalovirus. J Infect Dis 1985;152:755–759.Google Scholar
6.Huang, ES, Alford, CA, Reynolds, DW, Stagno, S, Pass, RFMolecular epidemiology of cytomegalovirus infections in women and their infants. N Engl J Med 1980;303:958–962.Google Scholar
7.Chou, S. Acquisition of donor strains of cytomegalovirus by renal transplant recipients. N Engl J Med 1986;22:1418–1423.Google Scholar
8.Chou, S. Differentiation of cytomegalovirus strains by restriction analysis of DNA sequences amplified from clinical specimens. J Infect Dis 1990;162:738–742.Google Scholar
9.Zaia, JA, Gallez-Hawkins, G, Churchill, MA. Comparative analysis of human cytomegalovirus a sequence in multiple clinical isolates by using poly-merase chain reaction and restriction fragment polymorphism assays. J Clin Microbiol 1990;28:2602–2607.Google Scholar
10.Bale, JEO'Neil, ME, Fowler, SS, Murph, JR. Analysis of acquired human cytomegalovirus infections by polymerase chain reaction. J Clin Microbiol 1993;31:2043–2048.Google Scholar
11.Souza, IE, Nicholson, D, Matthey, S, et al. Detection of human cytomega-in peripheral blood leukocytes by the polymerase chain reaction and a nonradioactive probe. Diagn Microbiol Infect Dis 1994;20:13–19.Google Scholar
12.Haugen, TH, Alden, B, Matthey, S, Nicholson, D. Restriction enzyme fragment length polymorphism of amplified herpes simplex Type-1 DNA provide epidemiologic information. Diagn Microbiol Infect Dis 1993;17:129–133.Google Scholar
13.Winston, DJ, Ho, WG, Champlin, RE. Cytomegalovirus infections after allogeneic bone marrow transplantation. Infect Dis 1990;12(suppl):776S–792s.Google Scholar
14.Williams, SD, Kwok, S. Laboratory Diagnosis of Viral Infections. 2nd ed. New York, NY: Marcel Dekker, Inc; 1992:147.Google Scholar
15.Souza, IE, Greg, A, Pfab, D, et al. Intrafamilial CMV transmission: relationship to congenital infection. Presented at the 6th International Congress for Infectious Diseases; April 2630, 1994; Prague, Czech Republic. Abstract 579.Google Scholar
16.Souza, IE, Benson, RMurph, J, et al. Polymerase chain reaction analysis of cytomegalovirus strains from congenitally-infected infants. Presented at the 34th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC); October 4-7,1994; Orlando, Florida. Abstract J21.Google Scholar
17.Sokol, DM, Demmler, GJ, Buffone, GJ. Rapid epidemiologic analysis of cytomegalovirus by using polymerase chain reaction amplifcation of the L-S junction J Clin Microbiol 1991;30:839–844.Google Scholar