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Evaluation of epiphytic microbiota in red clover and alfalfa on silage fermentation products, bacterial community diversity and functionality of oat

Published online by Cambridge University Press:  29 April 2024

Siran Wang Open the ORCID record for Siran Wang [Opens in a new window] ,
Junfeng Li ,
Jie Zhao ,
Zhihao Dong ,
Mudasir Nazar ,
Niaz Ali Kaka ,
Ziqun Lin  and
Tao Shao
Siran Wang
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Junfeng Li
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Jie Zhao
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Zhihao Dong
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Mudasir Nazar
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Niaz Ali Kaka
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Ziqun Lin
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Tao Shao*
Affiliation:
Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
*
Corresponding author: Tao Shao; Email: taoshaolan@163.com
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Abstract

The purpose of this experiment was to evaluate the contribution of epiphytic microbiota on alfalfa (AL), oat (OT), and red clover (RC) to ensiling characteristics and bacterial community diversity of oat. With the irradiation of γ-ray, sterile OT (~233 g/kg dry matter (DM)) was inoculated by sterile water (STOT), epiphytic microbiota from OT (OTOT), AL (OTAL) and RC (OTRC), respectively. Triplicate silage-bags for each treatment were sampled after different days (1, 3, 7, 15, 30 and 60) of fermentation, respectively. Similar chemical compositions were found between fresh oat and STOT. Lower (P < 0.05) contents of ammonia nitrogen (NH3-N) and higher (P < 0.05) accumulation of lactic acid were found in OTAL compared with OTRC and OTOT on day 3. The greatest (P < 0.05) NH3-N, acetic acid concentrations and pH and the lowest (P < 0.05) concentration of lactic acid were found in OTRC on day 60. After 3 days of ensiling, Lactobacillus accounted for a big proportion in OTAL and OTOT, and Hafnia-Obesumbacterium was predominant in OTRC. The bacterial communities in OTAL and OTOT had lower (P < 0.05) abundances of ‘Genetic Information Processing’ than OTRC after 3 days. Overall, the composition, diversity, and activity of epiphytic microbiota can notably influence the ensiling characteristics of forage oat. The lactic acid bacteria (hetero-fermentative type) and Enterobacteriaceae species played an important role in producing ethanol contents during the ensiling of forage oat.

Keywords

bacterial communityensilingfermentation productoatsilage
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , First View , pp. 1 - 9
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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References

Annicchiarico, P, Barrett, B, Brummer, EC, Julier, B and Marshall, AH (2015) Achievements and challenges in improving temperate perennial forage legumes. Critical Reviews in Plant Sciences 34, 327–380.Google Scholar
Borreani, G, Tabacco, E, Schmidt, RJ, Holmes, BJ and Muck, RE (2018) Silage review: factors affecting dry matter and quality losses in silages. Journal of Dairy Science 101, 3952–3979.Google Scholar
Broderick, GA and Kang, JH (1980) Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science 63, 64–75.Google Scholar
Buxton, DR, Muck, RE, Harrison, JH, Buxton, DR and O'Kiely, P (2003) Preharvest plant factors affecting ensiling. Agronomy Monograph 42, c5.Google Scholar
Duniere, L, Sindou, J, Chaucheyras-Durand, F, Chevallier, I and Thévenot-Sergentet, D (2013) Silage processing and strategies to prevent persistence of undesirable microorganisms. Animal Feed Science and Technology 182, 1–15.Google Scholar
Duniere, L, Xu, S, Long, J, Elekwachi, C, Wang, Y, Turkington, K, Forster, R and McAllister, TA (2017) Bacterial and fungal core microbiomes associated with small grain silages during ensiling and aerobic spoilage. BMC Microbiology 17, 50.Google Scholar
Graf, K, Ulrich, A, Idler, C and Klocke, M (2016) Bacterial community dynamics during ensiling of perennial ryegrass at two compaction levels monitored by terminal restriction fragment length polymorphism. Journal of Applied Microbiology 120, 1479–1491.Google Scholar
Guan, H, Yan, Y, Li, X, Li, X, Shuai, Y, Feng, G and Zhang, X (2018) Microbial communities and natural fermentation of corn silages prepared with farm bunker-silo in Southwest China. Bioresource Technology 265, 282–290.Google Scholar
Kathrin, PA, Bernd, W, Rolf, D and Peter, M (2015) Tax4fun: predicting functional profiles from metagenomic 16S rRNA data. Bioinformatics (Oxford, England) 31, 2882–2884.Google Scholar
Keshri, J, Chen, Y, Pinto, R, Kroupitski, Y, Weinberg, ZG and Sela Saldinger, S (2018) Microbiome dynamics during ensiling of corn with and without Lactobacillus plantarum inoculant. Applied Microbiology and Biotechnology 102, 4025–4037.Google Scholar
Khan, NA, Tewoldebrhan, TA, Zom, RLG, Cone, JW and Hendriks, WH (2012) Effect of corn silage harvest maturity and concentrate type on milk fatty acid composition of dairy cows. Journal of Dairy Science 95, 1472–1483.Google Scholar
Krishnamoorthy, U, Muscato, TV, Sniffen, CJ and Van Soest, PJ (1982) Nitrogen fractions in selected feedstuffs. Journal of Dairy Science 65, 217–225.Google Scholar
Kung, L Jr, Shaver, RD, Grant, RJ and Schmidt, RJ (2018) Silage review: interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science 101, 4020–4033.Google Scholar
Li, DX, Ni, KK, Zhang, YC, Lin, YL and Yang, FY (2018) Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality. Journal of Integrative Agriculture 17, 2768–2782.Google Scholar
Li, P, Tang, X, Liao, C, Li, M, Chen, L, Lu, G, Huang, X, Chen, C and Gou, W (2021) Effects of additives on silage fermentation characteristic and in vitro digestibility of perennial oat at different maturity stages on the Qinghai Tibetan. Microorganisms 9, 2403.Google Scholar
Ma, SS, Fang, C, Sun, XX, Han, LJ, He, XQ and Huang, GQ (2018) Bacterial community succession during pig manure and wheat straw aerobic composting covered with a semi-permeable membrane under slight positive pressure. Bioresource Technology 259, 221–227.Google Scholar
Mao, T and Kanehisa, M (2012) Using the KEGG database resource. Current Protocols in Bioinformatics 38, 1.12.1–1.12.43.Google Scholar
McDonald, P, Henderson, AR and Heron, S (1991) The Biochemistry of Silage. Abersytwyth, UK: Chalcombe Publications.Google Scholar
McDonald, P, Edwards, RA, Greenhalgh, JFD and Morgan, CA (2002) Animal Nutrition, 6th Edn. Harlow: Pearson Education Limited, pp. 1–693.Google Scholar
Nazar, M, Wang, S, Zhao, J, Dong, Z, Li, J, Ali Kaka, N and Shao, T (2021) Abundance and diversity of epiphytic microbiota on forage crops and their fermentation characteristic during the ensiling of sterile Sudan grass. World Journal of Microbiology & Biotechnology 37, 27.Google Scholar
Ogunade, IM, Jiang, Y, Pech Cervantes, AA, Kim, DH, Oliveira, AS, Vyas, D, Weinberg, ZG, Jeong, KC and Adesogan, AT (2018) Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: effects of Escherichia coli O157:H7 and silage additives. Journal of Dairy Science 101, 2048–2059.Google Scholar
Playne, MJ and McDonald, P (1966) The buffering constituents of herbage and of silage. Journal of the Science of Food and Agriculture 17, 264–268.Google Scholar
Sun, L, Jiang, Y, Ling, Q, Na, N, Xu, H, Vyas, D, Adesogan, AT and Xue, Y (2021) Effects of adding pre-fermented fluid prepared from red clover or lucerne on fermentation quality and in vitro digestibility of red clover and Lucerne silages. Agriculture 11, 454.Google Scholar
Tahir, M, Li, J, Xin, Y, Wang, T, Chen, C, Zhong, Y, Zhang, L, Liu, H, He, Y, Wen, X and Yan, Y (2023) Response of fermentation quality and microbial community of oat silage to homofermentative lactic acid bacteria inoculation. Frontiers in Microbiology 13, 1091394.Google Scholar
Thomas, TA (1977) An automated procedure for the determination of soluble carbohydrates in herbage. Journal of the Science of Food and Agriculture 28, 639–642.Google Scholar
Wang, Y, Chen, XY, Wang, C, He, LW, Zhou, W and Yang, FY (2019) The bacterial community and fermentation quality of mulberry (Morus alba) leaf silage with or without Lactobacillus casei and sucrose. Bioresource Technology 293, 122059.Google Scholar
Wang, S, Sun, Y, Zhao, J, Dong, Z, Li, J, Nazar, M and Shao, T (2020a) Assessment of inoculating various epiphytic microbiota on fermentative profile and microbial community dynamics in sterile Italian ryegrass. Journal of Applied Microbiology 129, 509–520.Google Scholar
Wang, S, Zhao, J, Dong, Z, Li, J and Shao, T (2020b) Sequencing and microbiota transplantation to determine the role of microbiota on the fermentation type of oat silage. Bioresource Technology 309, 123371.Google Scholar
Wang, S, Li, J, Zhao, J, Dong, Z and Shao, T (2022) Exploring the ensiling characteristics and bacterial community of red clover inoculated with the epiphytic bacteria from temperate gramineous grasses. Journal of Applied Microbiology 132, 177–188.Google Scholar
Zhao, S, Yang, F, Wang, Y, Fan, X and Wang, Y (2021) Dynamics of fermentation parameters and bacterial community in high-moisture alfalfa silage with or without lactic acid bacteria. Microorganisms 9, 1225.Google Scholar