Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-06-02T06:18:48.062Z Has data issue: false hasContentIssue false
  • English
  • Français

Oxidation and metabolism of linoleic acid in fed and fasted sheep

Published online by Cambridge University Press:  27 March 2009

D. B. Lindsay  and
W. M. F. Leat
D. B. Lindsay
Affiliation:
A. R. C. Institute of Animal Physiology, Babraham, Cambridge CBe 4AT
W. M. F. Leat
Affiliation:
A. R. C. Institute of Animal Physiology, Babraham, Cambridge CBe 4AT
Get access Rights & Permissions [Opens in a new window]

Summary

Using continuous infusion techniques the entry rate of linoleic acid and its contribution to carbon dioxide production has been studied in fed and fasted sheep. The entry rate of linoleic acid in fasted sheep (0·052 mg/min/kg) was considerably less than that of stearic acid (0·432 mg/min/kg), and the percentage of total carbon dioxide production derived from the oxidation of linoleic acid (0·4%) was also markedly less than that from stearic acid (10·13%).

On starvation there was a preferential mobilization of palmitic, stearic and oleic acids relative to linoleic acid.

The infused 14C-labelled linoleic acid was initially incorporated into plasma phospholipids, with maximal radioactivity at about 1 day post-infusion, and subsequently into plasma cholesteryl esters with a maximum at 2 days post-infusion. The incorporation of 14C-labelled stearic acid into plasma phospholipids was only 25–30% that of linoleic acid, with negligible incorporation into cholesteryl esters.

Possible explanations for ruminant animals being able to exist on a very low intake of linoleic acid are discussed. In essence, there is a discrimination against the use of linoleic acid as an energy source, but the mechanisms involved do not appear to be unique to ruminant animals.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 89 , Issue 1 , August 1977 , pp. 215 - 221
Copyright
Copyright © Cambridge University Press 1977

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Annison, E. F., Brown, R. E., Leng, R. A., Lindsay, D. B. & West, C. E. (1967). Rates of entry and oxidation of acetate, glucose, D(—)-β-hydroxybutyrate, palmitate, oleate and stearate and rates of production and oxidation of propionate and butyrate in fed and starved sheep. Biochemical Journal 104, 135–47.CrossRefGoogle ScholarPubMed
Bowyer, D. E., Leat, W. M. F., Howard, A. N. & Gresham, G. A. (1963). The determination of the fatty acid composition of serum lipids separated by thin layer chromatography; and a comparison with column chromatography. Biochimica et biophysica Acta 70, 423–31.CrossRefGoogle Scholar
Dawson, R. M. C. & Kemp, P. (1970). Biohydrogenation of dietary fats in ruminants. In Physiology of Digestion and Metabolism in the Ruminant (ed. Phillipson, A. T.), pp. 504–18. Newcastle-upon-Tyne: Oriel Press.Google Scholar
Duncombe, W. G. (1964). The colorimetric micro determination of non-esterified fatty acids in plasma. Clinica Ghimica Acta 9, 122–5.CrossRefGoogle ScholarPubMed
Folch, J., Lees, M. & Sloane Stanley, G. H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.CrossRefGoogle ScholarPubMed
Glomset, J. A. (1968). The plasma lecithin: cholesterol acyltransferase reaction. Journal of Lipid Research 9, 155–67.CrossRefGoogle Scholar
Holman, R. T. (1968). Essential fatty acid deficiency. In Progress in the Chemistry of Fats and Other Lipids, vol. IX, pp. 279–348. Oxford: Pergamon Press.Google Scholar
Infield, J. M. & Annison, E. F. (1973). The metabolism of palmitic, stearic, oleic and linoleio acids in broiler chickens. British Journal of Nutrition 30, 545–54.CrossRefGoogle Scholar
Leat, W. M. F. (1966). Fatty acid composition of the plasma lipids of newborn and maternal ruminants. Biochemical Journal 98, 598–603.CrossRefGoogle ScholarPubMed
Leat, W. M. F. & Ford, E. J. H. (1966). Utilization of free fatty acids by starved and pregnant sheep. Biochemical Journal 101, 317–22.CrossRefGoogle ScholarPubMed
Leat, W. M. F. & Harrison, F. A. (1972). Intake and absorption of essential fatty acids by the sheep. Proceedings of the Nutrition Society 31, 70A.Google ScholarPubMed
Leat, W. M. F., Kubasek, F. O. T. & Buttress, N. (1976). Plasma lipoproteins of lambs and sheep. Quarterly Journal of Experimental Physiology 61, 193–202.CrossRefGoogle ScholarPubMed
Leat, W. M. F., Lindsay, D. B. & Valerio, G. (1975). Oxidation and metabolism of linoleic acid in the sheep. Proceedings of the Nutrition Society 34, 88A.Google ScholarPubMed
Lindsay, D. B. (1976). Fatty acids as energy sources. Proceedings of the Nutrition Society 34, 241–8.CrossRefGoogle Scholar
West, C. E. & Annison, E. F. (1964). Metabolism of palmitate in sheep. Biochemical Journal 92, 673–8.CrossRefGoogle ScholarPubMed
Young, B. A., Kerrigan, B. & Christopherson, B. J. (1975). A versatile respiratory pattern analyser for studies of energy metabolism of livestock. Canadian Journal of Animal Science 55, 17–22.CrossRefGoogle Scholar
Zixveksmit, D. B., Entenman, C. & Fishier, M. C. (1943). On the calculation of ‘turnover time’ and ‘turnover rate’ from experiments involving the use of labelling agents. Journal of General Physiology 26, 325–31.Google Scholar