Hostname: page-component-848d4c4894-x24gv Total loading time: 0 Render date: 2024-05-31T22:28:13.839Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of low dietary aflatoxin B1 on broiler liver concentration without and with Mycosorb® toxin binder

Published online by Cambridge University Press:  05 December 2013

C.A. Moran ,
J. Apajalahti ,
A. Yiannikouris ,
S. Ojanperä  and
H. Kettunen
C.A. Moran*
Affiliation:
Alltech France, 25 rue Greffulhe, 92300 Levallois-Perret, France
J. Apajalahti
Affiliation:
Alimetrics Ltd., Koskelontie 19 B, 02920 Espoo, Finland
A. Yiannikouris
Affiliation:
Alltech Inc, 3031 Catnip Hill Pike, Nicholasville, 40356 KY, USA
S. Ojanperä
Affiliation:
Alimetrics Ltd., Koskelontie 19 B, 02920 Espoo, Finland
H. Kettunen
Affiliation:
Alimetrics Ltd., Koskelontie 19 B, 02920 Espoo, Finland
*
*Corresponding author:cmoran@alltech.com
Get access Rights & Permissions [Opens in a new window]

Summary

A pilot study was conducted to evaluate the suitability of hepatic aflatoxin (AFB1) concentration as a biomarker to assess the in vivo efficacy of a mycotoxin binder in poultry when AFB1 dietary concentrations are low. Diets containing low doses of AFB1 without or with Mycosorb® (MTB), a mycotoxin binder, were fed to broilers from 7 to 21 days of age. The accumulation of AFB1 in liver was measured by high performance liquid chromatography with a detection limit of 5 ng/kg liver. In response to 10 and 50 µg AFB1/kg feed, hepatic AFB1 accumulation was 27 and 145 ng AFB1/kg liver, respectively. At each dietary concentration of AFB1, the inclusion of 5 g MTB/kg of feed reduced (P < 0.1 for 10 μg AFB1/kg feed and P < 0.05 for 50 μg AFB1/kg feed) hepatic AFB1 accumulation by at least 50%. These results suggest that hepatic AFB1 concentration is a suitable biomarker for evaluating mycotoxin binder efficacy in poultry fed the EU maximum dietary concentration of 10 µg of AFB1/kg feed.

Keywords

Aflatoxin B1mycotoxinbroilerliverMycosorb®
Type
Pilot Study
Information
Journal of Applied Animal Nutrition , Volume 2 , 2013 , e4
Copyright
Copyright © Cambridge University Press and Journal of Applied Animal Nutrition Ltd. 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bintvihok, A. & Kositcharoenkul, S. (2006) Effect of dietary calcium propionate on performance, hepatic enzyme activities and aflatoxin residues in broilers fed a diet containing low levels of aflatoxin B1. Toxicon, 47:41–46.Google Scholar
EFSA (2010) EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP); Statement on the establishment of guidelines for the assessment of additives from the functional group ‘substances for reduction of the contamination of feed by mycotoxins’. EFSA Journal, 8(7):1693–1700. doi:10.2903/j.efsa.2010.1693.Google Scholar
EFSA (2011) EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP); Technical guidance: Tolerance and efficacy studies in target animals. EFSA Journal, 9(5):2175–2189. doi:10.2903/j.efsa.2011.2175.Google Scholar
Magnoli, A.P., Monge, M.P., Miazzo, R.D., Cavaglieri, L.R., Magnoli, C.E., Merkis, C.I., Cristofolini, A.L., Dalcero, A.M. & Chiacchiera, S.M. (2011) Effect of low levels of aflatoxin B1 on performance, biochemical parameters, and aflatoxin B1 in broiler liver tissues in the presence of monensin and sodium bentonite. Poultry Science, 90: 48–58.Google Scholar
Tavcar-Kalcher, G., Vrtac, K., Pestevsek, U. & Vengust, A. (2007) Validation of the procedure for the determination of aflatoxin B1 in animal liver using immunoaffinity columns and liquid chromatography with postcolumn derivatisation and fluorescence detection. Food Control, 18: 333–337.Google Scholar
Yunus, A.W., Razzazi-Fazeli, E. & Bohm, J. (2011) Aflatoxin B1 in affecting broiler's performance, immunity, and gastrointestinal tract: A review of history and contemporary issues. Toxins, 3, 566–590.CrossRefGoogle ScholarPubMed