Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-06-02T08:56:30.034Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of fibroblast growth factors in bovine cheese whey

Published online by Cambridge University Press:  01 June 2009

Mary-Louise Rogers ,
David A. Belford ,
Geoffrey L. Francis  and
F. John Ballard
Mary-Louise Rogers
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
David A. Belford
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
Geoffrey L. Francis
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
F. John Ballard
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
Get access Rights & Permissions [Opens in a new window]

Summary

Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin-sepharose column and eluted stepwise with 0·8, 1·2 and 2·0 M-NH4HC03. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1·2 M-eluate and basic FGF in the 2·0 M-eluate, but neither acidic nor basic FGF was detected in the 0·8 M-fraction. Quantitative radioreceptor assays indicated 5·8 ng of acidic FGF-like activity and 19·8 ng of basic FGF-like activity per 1 whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 3 , August 1995 , pp. 501 - 507
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Baird, A. & Bohlen, P. A. 1990 Fibroblast growth factors. In Peptide Growth Factors, vol. 1, pp. 369–417 (Eds Sporn, M. C. and Roberts, A. B.). New York: Springer-VerlagGoogle Scholar
Barraclough, R., Fernig, D. G., Rudland, P. S. & Smith, J. A. 1990 Synthesis of basic fibroblast growth factor upon differentiation of rat mammary epithelial to myo-epithelial-like cells in culture. Journal of Cellular Physiology 144 333–344CrossRefGoogle ScholarPubMed
Brigstock, D., Klagsbrun, M., Sasse, J., Farber, P. A. & Iberg, N. 1990 Species-specific high molecular weight forms of basic fibroblast growth factor. Growth Factors 4 45–52CrossRefGoogle ScholarPubMed
Brown, K. D. & Blakeley, D. M. 1984 Partial purification and characterization of a growth factor present in goat's colostrum. Similarities with platelet-derived growth factor. Biochemical Journal 219 609–617CrossRefGoogle ScholarPubMed
Carpenter, G. & Wahl, M. I. 1990 The epidermal growth factor. In Handbook of Experimental Pharmacology, vol. 95, pp. 69–171 (Eds Sporn, M. C. and Roberts, A. B.). Berlin: Springer-VerlagGoogle Scholar
Derouiche, A. F., Legrand, C., Bour, J. M., Capiaumont, J., Gelot, M. A., Dousset, B., Belleville, F., Nabet, P. & Linden, G. 1990 [Biochemical aspects of whey fractions capable of monitoring hybridoma proliferation: comparison with fetal calf serum.] Lait 70 313–324CrossRefGoogle Scholar
Ferrara, N. & Henzel, W. J. 1989 Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. Biochemical and Biophysical Research Communications 161 851–858CrossRefGoogle ScholarPubMed
Francis, G. L., Upton, F. M., Ballard, F. J., McNeil, K. A. & Wallace, J. C. 1988 Insulin-like growth factors 1 and 2 in bovine colostrum: sequences and biological activities compared with those of a potent truncated form. Biochemical Journal 251 95–103CrossRefGoogle ScholarPubMed
Gospodarowicz, D., Ferrara, N., Schweigerer, L. & Neufeld, G. 1987 Structural characterization and biological functions of fibroblast growth factor. Endocrine Reviews 8 95–114CrossRefGoogle ScholarPubMed
Jin, Y., Cox, D. A., Knecht, R., Raschdorf, F. & Cerletti, N. 1991 Separation, purification, and sequence identification of TGF-β1 and TGF-β2 from bovine milk. Journal of Protein Chemistry 10 565–575CrossRefGoogle Scholar
Ke, Y., Fernio, D. G., Wilkinson, M. C., Winstanley, J. H. R., Smith, J. A., Rudland, P. S. & Barraclough, R. 1993 The expression of basic fibroblast growth factor and its receptor in cell lines derived from normal human mammary gland and a benign mammary lesion. Journal of Cell Science 106 135–143CrossRefGoogle Scholar
Klagsbrun, M. 1989 The fibroblast growth factor family: structural and biological properties. Progress in Growth Factor Research 1 207–235CrossRefGoogle ScholarPubMed
Klagsbrun, M. 1992 Mediators of angiogenesis: the biological significance of basic fibroblast growth factor (bFGF)-heparin and heparan sulfate interactions. Seminars in Cancer Biology 3 81–87Google ScholarPubMed
Klagsbrun, M. & Neumann, J. 1979 The serum-free growth of Balb/c 3T3 cells in medium supplemented with bovine colostrum. Journal of Supramolecular Structure 11 349–359CrossRefGoogle ScholarPubMed
Lobb, R. R., Harper, J. W. & Fett, J. W. 1986 Purification of heparin-binding growth factors. Analytical Biochemistry 154 1–14CrossRefGoogle ScholarPubMed
Moscatelli, D. 1987 High and low affinity binding sites for basic fibroblast growth factor on cultured cells:absence of a role for low affinity binding in the stimulation of plasminogen activator production by bovine capillary endothelial cells. Journal of Cellular Physiology 131 123–130CrossRefGoogle ScholarPubMed
Neufeld, G. & Gospodarowicz, D. 1986 Basic and acidic fibroblast growth factors interact with the same cell surface receptors. Journal of Biological Chemistry 261 5631–5637CrossRefGoogle ScholarPubMed
Oliver, M. H., Harrison, N. K., Bishop, J. E., Cole, P. J. & Laurent, G. J. 1989 A rapid and convenient assay for counting cells cultured in microwell plates: application for assessment of growth factors. Journal of Cell Science 92 513–518CrossRefGoogle ScholarPubMed
Read, L. C., Upton, F. M., Francis, G. L., Wallace, J. C., Dahlenberg, G. W. & Ballard, F. J. 1984 Changes in the growth-promoting activity of human milk during lactation. Pediatric Research 18 133–139CrossRefGoogle ScholarPubMed
Sandowski, Y., Peri, I. & Gertler, A. 1993 Partial purification and characterization of putative paracrine/autocrine bovine mammary epithelium growth factors. Livestock Production Science 35 35–48CrossRefGoogle Scholar
Van Obberghen-Schilling, E. & Pouysségur, J. 1983 Mitogen-potentiating action and binding characteristics of insulin and insulin-like growth factors in Chinese hamster fibroblasts. Experimental Cell Research 147 369–378CrossRefGoogle ScholarPubMed
Wadzinski, M. G., Folkman, J., Sasse, J., Ingber, D., Devey, K., Ingber, D. & Klagsbrun, M. 1987 Heparin-binding angiogenesis factors: detection by immunological methods. Clinical Physiology and Biochemistry 5 200–209Google ScholarPubMed