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Retinol determination in milk by HPLC and fluorescence detection

Published online by Cambridge University Press:  01 June 2009

Søren Krogh Jensen
Søren Krogh Jensen
Affiliation:
National Institute of Animal Science, Research Centre Foulum, Department of Animal Physiology and Biochemistry, DK-8830 Tjele, Denmark
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Summary

A simple and rapid method for determination of retinol in whole milk is described. Milk (400 mg) was diluted with a water–methanol–ethanol mixture (55:9:36 by vol.) and saponified with potassium hydroxide in a culture tube. Retinol was extracted into 10ml heptane-di-isopropyl ether (3:1) of which 100 μl was injected into the HPLC. Fluorescence detection ensured the specificity and sensitivity. The critical points in the analysis were the extraction of retinol from fatty samples and the instability of retinol towards photo-oxidation and air oxidation in alkaline solvents. There was an average recovery of 98·5 ± 2·4% and a CV of 3·6%.

Type
Original Articles
Information
Journal of Dairy Research , Volume 61 , Issue 2 , May 1994 , pp. 233 - 240
Copyright
Copyright © Proprietors of Journal of Dairy Research 1994

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References

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