Hostname: page-component-848d4c4894-75dct Total loading time: 0 Render date: 2024-06-02T04:07:40.725Z Has data issue: false hasContentIssue false
  • English
  • Français

Supplementing conjugated and non-conjugated L-methionine and acetate alters expression patterns of CSN2, proteins and metabolites related to protein synthesis in bovine mammary cells

Published online by Cambridge University Press:  02 March 2020

Seung-Woo Jeon ,
Jay Ronel Conejos ,
Jungeun Kim ,
Min-Jeong Kim ,
Jeong-Eun Lee ,
Baek-Seok Lee ,
Jin-Seung Park ,
Jun-Ok Moon ,
Jae-Sung Lee  and
Hong-Gu Lee
Seung-Woo Jeon
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Jay Ronel Conejos
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Jungeun Kim
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Min-Jeong Kim
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Jeong-Eun Lee
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Baek-Seok Lee
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Jin-Seung Park
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Jun-Ok Moon
Affiliation:
CJ CheilJedang Research Institute of Biotechnology, Suwon, Republic of Korea
Jae-Sung Lee
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
Hong-Gu Lee*
Affiliation:
Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
*
Author for correspondence: Hong-Gu Lee, Email: hglee66@konkuk.ac.kr
Get access Rights & Permissions [Opens in a new window]

Abstract

The experiments reported in this research paper aimed to determine the effect of supplementing different forms of L-methionine (L-Met) and acetate on protein synthesis in immortalized bovine mammary epithelial cell line (MAC-T cells). Treatments were Control, L-Met, conjugated L-Met and acetate (CMA), and non-conjugated L-Met and Acetate (NMA). Protein synthesis mechanism was determined by omics method. NMA group had the highest protein content in the media and CSN2 mRNA expression levels (P < 0.05). The number of upregulated and downregulated proteins observed were 39 and 77 in L-Met group, 62 and 80 in CMA group and 50 and 81 in NMA group from 448 proteins, respectively (P < 0.05). L-Met, NMA and CMA treatments stimulated pathways related to protein and energy metabolism (P < 0.05). Metabolomic analysis also revealed that L-Met, CMA and NMA treatments resulted in increases of several metabolites (P < 0.05). In conclusion, NMA treatment increased protein concentration and expression level of CSN2 mRNA in MAC-T cells compared to control as well as L-Met and CMA treatments through increased expression of milk protein synthesis-related genes and production of the proteins and metabolites involved in energy and protein synthesis pathways.

Keywords

acetateL-methionineomicsprotein synthesis
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 1 , February 2020 , pp. 70 - 77
Copyright
Copyright © Hannah Dairy Research Foundation 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Equal Contribution Status.

References

Appuhamy, JA, Knoebel, NA, Nayananjalie, WA, Escobar, J and Hanigan, MD (2012) Isoleucine and leucine independently regulate mTOR signaling and protein synthesis in MAC-T cells and bovine mammary tissue slices. Journal of Nutrition 142, 484–491.10.3945/jn.111.152595Google ScholarPubMed
Baumrucker, CR (1985) Amino acid transport systems in bovine mammary tissue. Journal of Dairy Science 68, 2436–2451.10.3168/jds.S0022-0302(85)81119-XGoogle ScholarPubMed
Berthiaume, R, Thivierge, MC, Patton, RA, Dubreull, P, Stevenson, M, McBride, BW and Lapierre, H (2006) Effect of ruminally protected methionine on splanchnic metabolism of amino acids in lactating dairy cows. Journal of Dairy Science 89, 1621–1634.Google ScholarPubMed
Bionaz, M, Hurley, WL and Loor, JJ (2012) Milk protein synthesis in the lactating mammary gland: insights from transcriptomics analyses. In Hurley, WL (ed.), Milk Protein. London, UK: InTech, p. 285–324.Google Scholar
Bruhat, A, Jousse, C, Wang, XZ, Ron, D, Ferrara, M and Fafournoux, P (1997) Amino acid limitation induces expression of CHOP, a CCAAT/enhancer binding protein-related gene, at both transcriptional and post-transcriptional levels. Journal of Biological Chemistry 272, 17588–17593.Google ScholarPubMed
Chilliard, Y and Doreau, M (1997) Influence of supplementary fish oil and rumen-protected methionine on milk yield and composition in dairy cows. Journal of Dairy Research 64, 173–179.Google ScholarPubMed
Deval, C, Chaveroux, C, Maurin, AC, Cherasse, Y, Parry, L, Carraro, V, Milenkovic, D, Ferrara, M, Bruhat, A, Jousse, C and Fafournoux, P (2009) Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways. FEBS Journal 276, 707–718.10.1111/j.1742-4658.2008.06818.xGoogle ScholarPubMed
Doepel, L, Pacheco, D, Kennelly, JJ, Hanigan, MD, Lopez, IF and Lapierre, H (2004) Protein synthesis as a function of amino acid supply. Journal of Dairy Science 87, 1279–1297.10.3168/jds.S0022-0302(04)73278-6Google ScholarPubMed
Englander, MT, Avinsb, JL, Fleisherb, RC, Liuc, B, Effraima, PR, Wang, J, Schultenc, K, Leyhf, TS, Gonzalez, RL Jr and Cornishb, VW (2015) The ribosome can discriminate the chirality of amino acids within its peptidyl-transferase center. Proceedings of the National Academy of Sciences USA 112 6038–6043.Google ScholarPubMed
Fagundes, MA, Blaser, SA, Yang, SY, Eun, JS and Moon, JO (2016) Effects of supplementing rumen-protected methionine on lactational performance of Holstein dairy cows during early and mid-lactation. Journal of Animal Science 94 (suppl 5), 349.Google Scholar
Griinari, JM, McGuire, MA, Dwyer, DA, Bauman, DE, Barbano, DM and House, WA (1997) The role of insulin in the regulation of protein synthesis in dairy cows. Journal of Dairy Science 80, 2361–2371.Google ScholarPubMed
Ishihama, Y, Oda, Y, Tabata, T, Sato, T, Nagasu, T, Rappsilber, J and Mann, M (2005) Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein. Molecular and Cellular Proteomics 4, 1265–1272.Google ScholarPubMed
Jousse, C, Averous, J, Bruhat, A, Carraro, V, Mordier, S and Fafournoux, P (2004) Amino acids as regulators of gene expression: molecular mechanisms. Biochemical and Biophysical Research Communications 313, 447–452.Google ScholarPubMed
Kowalski, ZM, Pisulewski, PM and Gorgulu, M (2003) Effects of protected methionine and variable energy supply on lactational responses in dairy cows fed grass silage-based diets. Journal of Animal and Feed Sciences 12, 451–464.Google Scholar
Leonardi, C, Stevenson, M and Armentano, LE (2003) Effect of two levels of crude protein and methionine supplementation on performance of dairy cows. Journal of Dairy Science 86, 4033–4042.Google ScholarPubMed
Limin, L, Xuejun, G, Qingzhang, L, Jianguo, H, Rong, L and Huiming, L (2012) Comparative phosphoproteomics analysis of the effects of L-methionine on dairy cow mammary epithelial cells. Canadian Journal of Animal Science 92, 433–442.Google Scholar
Livak, KJ and Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402–408.Google Scholar
Lu, LM, Li, QL, Huang, JG and Gao, XJ (2013) Proteomic and functional analyses reveal MAPK1 regulates milk protein synthesis. Molecules 18, 263–275.Google Scholar
Metayer, S, Seiliez, I, Collin, A, Duchene, S, Mercier, Y, Geraert, PA and Tesseraud, S (2008) Mechanisms through which sulfur amino acids control protein metabolism and oxidative status. Journal of Nutritional Biochemistry 19, 207–215.10.1016/j.jnutbio.2007.05.006Google ScholarPubMed
Nan, X, Bu, D, Li, X, Wang, J, Wei, H, Hu, H, Zhou, L and Loor, JJ (2014) Ratio of lysine to methionine alters expression of genes involved in protein transcription and translation and mTOR phosphorylation in bovine mammary cells. Physiological Genomics 46, 268–275.Google ScholarPubMed
National Research Council (2001) Nutrient Requirements of Dairy Cattle, 7th rev. Edn., Washington, DC: National Academy Press.Google Scholar
Nicholls, C, Li, H and Liu, JP (2012) GAPDH: a common enzyme with uncommon functions. Clinical and Experimental Pharmacology and Physiology 39, 674–679.10.1111/j.1440-1681.2011.05599.xGoogle ScholarPubMed
Orellana, RA, Jeyapalan, A, Escobar, J, Frank, JW, Nguyen, HV, Suryawan, A and Davis, TA (2007) Amino acids augment muscle protein synthesis in neonatal pigs during acute endotoxemia by stimulating mTOR-dependent translation initiation. American Journal of Physiology-Endocrinology and Metabolism 293, E1416–E1425.Google ScholarPubMed
Park, C, Yun, S, Lee, SY, Park, K and Lee, J (2012) Metabolic profiling of Klebsiella oxytoca: evaluation of methods for extraction of intracellular metabolites using UPLC/Q-TOF-MS. Applied Biochemistry and Biotechnology 167, 425–438.Google ScholarPubMed
Pietrocola, F, Galluzzi, L, Bravo-San Pedro, JM, Madeo, F and Kroemer, G (2015) Acetyl coenzyme A: a central metabolite and second messenger. Cell Metabolism 21, 805–821.Google ScholarPubMed
Proud, CG (2007) Signalling to translation: how signal transduction pathways control the protein synthetic machinery. Biochemical Journal 403, 217–234.10.1042/BJ20070024Google ScholarPubMed
Reynolds, CK, Harmon, DL and Cecava, MJ (1994) Absorption and delivery of nutrients for protein synthesis by portal-drained viscera. Journal of Dairy Science 77, 2787–2808.Google ScholarPubMed
Richter, EA and Ruderman, NB (2009) AMPK And the biochemistry of exercise: implications for human health and disease. Biochemical Journal 418, 261–275.Google ScholarPubMed
Rulquin, H and Delaby, L (1997) Effects of the energy balance of dairy cows on lactational responses to rumen-protected methionine. Journal of Dairy Science 80, 2513–2522.Google ScholarPubMed
Sabine, JR and Johnson, BC (1963) Acetate metabolism in the ruminant. Journal of Biological Chemistry 239, 89–93.Google Scholar
Sarbassov, DD, Ali, SM and Sabatini, DM (2005) Growing roles for the mTOR pathway. Current Opinion In Cell Biology 17, 596–603.Google ScholarPubMed
Schmidt, GH (1966) Effect of insulin on yield and composition of milk of dairy cows. Journal of Dairy Science 49, 381–385.10.3168/jds.S0022-0302(66)87878-5Google ScholarPubMed
Shennan, DB and Peaker, M (2000) Transport of milk constituents by the mammary gland. Physiological Reviews 80, 925–951.10.1152/physrev.2000.80.3.925Google ScholarPubMed
Socha, MT, Putnam, DE, Garthwaite, BD, Whitehouse, NL, Kierstead, NA, Schwab, CG, Ducharme, GA and Robert, JC (2005) Improving intestinal amino acid supply of pre- and postpartum dairy cows with rumen-protected methionine and lysine. Journal of Dairy Science 88, 1113–1126.Google ScholarPubMed
Toledo, MZ, Baez, GM, Garcia-Guerra, A, Lobos, NE, Guenther, JN and Trevisol, E (2017) Effect of feeding rumen-protected methionine on productive and reproductive performance of dairy cows. PLoS ONE 12, e0189117. https://doi.org/10.1371/journal.pone.0189117.Google ScholarPubMed
Wang, T, Lim, JN, Bok, JD, Kim, JH, Kang, SK, Lee, SB, Hwang, JH, Lee, KH, Kang, HS, Choi, YJ, Kim, EJ and Lee, HG (2014) Association of protein expression in isolated milk epithelial cells and cis-9, trans-11 CLA concentrations in milk from dairy cows. Journal of the Science of Food and Agriculture 94, 1835–1843.Google Scholar
Wang, T, Lee, SB, Hwang, JH, Lim, JN, Jung, US, Kim, MJ, Kang, HS, Choi, SH, Lee, JS, Roh, SG and Lee, HG (2015) Proteomic analysis reveals PGAM1 altering cis-9, trans-11 conjugated linoleic acid synthesis in bovine mammary gland. Lipids 50, 469–481.Google ScholarPubMed
Supplementary material: PDF

Jeon et al. supplementary material

Jeon et al. supplementary material

Download Jeon et al. supplementary material(PDF)
PDF 438.7 KB