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Leptin administration during lactation leads to different nutritional, biometric, hemodynamic, and cardiac outcomes in prepubertal and adult female Wistar rats

Published online by Cambridge University Press:  01 February 2021

Karyne Pollo de Souza
Affiliation:
Laboratory of Experimental Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil
Samuel de Sousa Pedro
Affiliation:
Laboratory of Experimental Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil
Nazareth Novaes Rocha
Affiliation:
Laboratory of Experimental Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil
Emiliana Barbosa Marques
Affiliation:
Laboratory of Experimental Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil
Christianne Bretas Vieira Scaramello*
Affiliation:
Laboratory of Experimental Pharmacology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil
*
Address for correspondence: Christianne Bretas Vieira Scaramello, Laboratory of Experimental Pharmacology/Department of Physiology and Pharmacology, room 204A, Fluminense Federal University, Professor Hernani Pires de Melo street, 101, São Domingos, Niterói, Rio de Janeiro, 24.210-130, Brazil. Email: chrisbretas@gmail.com

Abstract

Literature reports that insults, such as hormonal disturbances, during critical periods of development may modulate organism physiology and metabolism favoring cardiovascular diseases (CVDs) later in life. Studies show that leptin administration during lactation leads to cardiovascular dysfunction in young and adult male Wistar rats. However, there are sex differences regarding CVD. Thus, the present work aimed to investigate neonatal leptin administration’s consequences on different outcomes in female rats at prepubertal and adult age. Newborn Wistar female rats were divided into two groups, Leptin and Control, receiving daily subcutaneous injections of this adipokine (8 μg/100 g) or saline for the first 10 of 21 d of lactation. Nutritional, biometric, hemodynamic, and echocardiographic parameters, as well as maximal effort ergometer performance, were determined at postnatal days (PND) 30 and 150. Leptin group presented lower food intake (p = 0.0003) and higher feed efficiency (p = 0.0058) between PND 21 and 30. Differences concerning echocardiographic parameters revealed higher left ventricle internal diameter (LVID) in systole (p = 0.0051), as well as lower left ventricle ejection fraction (LVEF) (p = 0.0111) and fractional shortening (FS) (p = 0.0405) for this group at PND 30. Older rats treated with leptin during lactation presented only higher LVID in systole (p = 0.0270). Systolic blood pressure and maximum effort ergometer test performance was similar between groups at both ages. These data suggest that nutritional, biometric, and cardiac outcomes due to neonatal leptin administration in female rats are age-dependent.

Type
Original Article
Copyright
© The Author(s), 2021. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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