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Protein restriction during peripubertal period impairs endothelial aortic function in adult male Wistar rats

Published online by Cambridge University Press:  18 May 2023

Amanda Cristina de Souza ,
Deborah Gomes da Silva ,
Juliana da Silva Jezuíno ,
Anna Rebeka Oliveira Ferreira ,
Maiara Vanusa Guedes Ribeiro ,
Camila Borecki Vidigal ,
Kawane Fabricio Moura ,
Rafaela Pires Erthal ,
Paulo Cezar de Freitas Mathias  and
Glaura Scantamburlo Alves Fernandes Open the ORCID record for Glaura Scantamburlo Alves Fernandes [Opens in a new window]
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Amanda Cristina de Souza
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, State University of Londrina, Londrina, Brazil
Deborah Gomes da Silva
Affiliation:
Graduation Program of Physiological Sciences, Department of Physiological Sciences, State University of Londrina, Londrina, Brazil
Juliana da Silva Jezuíno
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, State University of Londrina, Londrina, Brazil
Anna Rebeka Oliveira Ferreira
Affiliation:
Department of Cell Biology and Genetics, Center of Biological Sciences, State University of Maringa, Maringa, Brazil
Maiara Vanusa Guedes Ribeiro
Affiliation:
Department of Cell Biology and Genetics, Center of Biological Sciences, State University of Maringa, Maringa, Brazil
Camila Borecki Vidigal
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, State University of Londrina, Londrina, Brazil
Kawane Fabricio Moura
Affiliation:
Graduation Program of Physiological Sciences, Department of Physiological Sciences, State University of Londrina, Londrina, Brazil
Rafaela Pires Erthal
Affiliation:
Department of General Biology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil
Paulo Cezar de Freitas Mathias
Affiliation:
Department of Cell Biology and Genetics, Center of Biological Sciences, State University of Maringa, Maringa, Brazil
Glaura Scantamburlo Alves Fernandes
Affiliation:
Graduation Program of Physiological Sciences, Department of Physiological Sciences, State University of Londrina, Londrina, Brazil Department of General Biology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil
Kesia Palma-Rigo
Affiliation:
Department of Cell Biology and Genetics, Center of Biological Sciences, State University of Maringa, Maringa, Brazil Adventist College of Parana, Ivatuba, Brazil
Graziela Scalianti Ceravolo*
Affiliation:
Department of Physiological Sciences, Center of Biological Sciences, State University of Londrina, Londrina, Brazil Graduation Program of Physiological Sciences, Department of Physiological Sciences, State University of Londrina, Londrina, Brazil
*
Corresponding author: Graziela Scalianti Ceravolo, Department of Physiological Sciences, Center of Biological Sciences, State University of Londrina, Londrina, Brazil. Email: gsceravolo@uel.br
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Abstract

Protein restriction during early phases of body development, such as intrauterine life can favor the development of vascular disorders. However, it is not known if peripubertal protein restriction can favor vascular dysfunction in adulthood. The present study aimed to evaluated whether a protein restriction diet during peripubertal period favors endothelial dysfunction in adulthood. Male Wistar rats from postnatal day (PND) 30 until 60 received a diet with either 23% protein (CTR group) or with 4% protein (LP group). At PND 120, the thoracic aorta reactivity to phenylephrine, acetylcholine, and sodium nitroprusside was evaluated in the presence or absence of: endothelium, indomethacin, apocynin and tempol. The maximum response (Rmax) and pD2 (-log of the concentration of the drug that causes 50% of the Rmax) were calculated. The lipid peroxidation and catalase activity were also evaluated in the aorta. The data were analyzed by ANOVA (one or two-ways and Tukey’s) or independent t-test; the results were expressed as mean ± S.E.M., p < 0.05. The Rmax to phenylephrine in aortic rings with endothelium were increased in LP rats when compared with the Rmax in CTR rats. Apocynin and tempol reduced Rmax to phenylephrine in LP aortic rings but not in CTR. The aortic response to the vasodilators was similar between the groups. Aortic catalase activity was lower and lipid peroxidation was greater in LP compared to CTR rats. Therefore, protein restriction during the peripubertal period causes endothelial dysfunction in adulthood through a mechanism related to oxidative stress.

Keywords

Undernutritionadolescenceoxidative stressaorta
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 14 , Issue 4 , August 2023 , pp. 451 - 458
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Copyright
© The Author(s), 2023. Published by Cambridge University Press in association with International Society for Developmental Origins of Health and Disease

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