Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-10-31T08:43:55.694Z Has data issue: false hasContentIssue false
  • English
  • Français

Energy metabolism of cells in the macula flava of the newborn vocal fold from the aspect of mitochondrial microstructure

Published online by Cambridge University Press:  25 June 2021

K Sato ,
S Chitose ,
K Sato ,
F Sato ,
T Ono  and
H Umeno
K Sato*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
S Chitose
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
K Sato
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
F Sato
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
T Ono
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
H Umeno
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
*
Author for correspondence: Dr Kiminori Sato, Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume830-0011, Japan E-mail: kimisato@oct-net.ne.jp Fax: +81 942 371 200
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective

Cells in the vocal fold of maculae flavae are likely to be tissue stem cells. Energy metabolism of the cells in newborn maculae flavae was investigated from the aspect of mitochondrial microstructure.

Method

Five normal newborn vocal folds were investigated under transmission electron microscopy.

Results

Mitochondria consisted of a double membrane bounded body containing matrices and a system of cristae. However, these membranes were ambiguous. In each mitochondrion, the lamellar cristae were sparse. Intercristal space was occupied by a mitochondrial matrix. Some mitochondria had fused to lipid droplets and rough endoplasmic reticulum, and both the mitochondrial outer and inner membranes had incarcerated and disappeared.

Conclusion

The features of the mitochondria of the cells in the newborn maculae flavae showed that their metabolic activity and oxidative phosphorylation were low. The metabolism of the cells in the newborn maculae flavae seems to be favourable to maintain the stemness and undifferentiation of the cells.

Keywords

Energy MetabolismMitochondriaStem CellsVocal CordsLarynxInfantNewborn
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 135 , Issue 9 , September 2021 , pp. 779 - 784
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Dr K Sato takes responsibility for the integrity of the content of the paper

References

Hirano, M. Phonosurgery. Basic and clinical investigations. Otologia (Fukuoka) 1975;21(suppl 1):239–60Google Scholar
Hirano, M, Kurita, S, Nakashima, T. Growth, development and aging of human vocal folds. In: Bless, DM, Abbs, JH, eds. Vocal Fold Physiology. San Diego, CA: College-Hill Press, 1983:22–43Google Scholar
Sato, K, Umeno, H, Nakashima, T. Vocal fold stem cells and their niche in the human vocal fold. Ann Otol Rhinol Laryngol 2012;121:798–803CrossRefGoogle ScholarPubMed
Kurita, T, Sato, K, Chitose, S, Fukahori, M, Sueyoshi, S, Umeno, H. Origin of vocal fold stellate cells in the human macula flava. Ann Otol Rhinol Laryngol 2015;124:698–705CrossRefGoogle ScholarPubMed
Sato, K, Chitose, S, Kurita, T, Umeno, H. Cell origin in the macula flava of the human newborn vocal fold. J Laryngol Otol 2016;130:650–5CrossRefGoogle ScholarPubMed
Sato, K, Chitose, S, Kurita, T, Umeno, H. Microenvironment of macula flava in the human vocal fold as a stem cell niche. J Laryngol Otol 2016;130:656–61CrossRefGoogle ScholarPubMed
Sato, K. The macula flava of the human vocal fold as a stem cell microenvironment. In: Birbrair, A, ed. Stem Cell Microenvironment and Beyond. Gewerbestrasse: Springer, 2017:171–86CrossRefGoogle Scholar
Sato, K. Tissue stem cells and the stem cell niche of the human vocal fold mucosa. In: Functional Histoanatomy of the Human Larynx. Singapore: Springer, 2018:165–7710.1007/978-981-10-5586-7_12CrossRefGoogle Scholar
Sato, K, Kurita, T, Chitose, S, Sato, K, Umeno, H, Yano, H. Distribution of label-retaining cells and their properties in the vocal fold mucosa. Laryngoscope Investig Otolaryngol 2018;4:76–82CrossRefGoogle ScholarPubMed
Sato, F, Chitose, S, Sato, K, Kurita, T, Sato, K, Umeno, H et al. Differentiation potential of the cells in the macula flava of the human vocal fold mucosa. Acta Histochem 2019;121:164–70CrossRefGoogle ScholarPubMed
Ghadially, FN. Mitochondria. In: Ultrastructural Pathology of the Cell and Matrix. London: Butterworths, 1988:191–328CrossRefGoogle Scholar
Sato, K, Umeno, H, Nakashima, T. Functional histology of the macula flava in the human vocal fold. Part 1: its role in the adult vocal fold. Folia Phoniatr Logop 2010;62:178–84CrossRefGoogle ScholarPubMed
Sato, K. Macula flava and vocal fold stellate cells of the human adult vocal fold. In: Functional Histoanatomy of the Human Larynx. Singapore: Springer, 2018:147–63CrossRefGoogle Scholar
Sato, K, Umeno, H, Nakashima, T. Functional histology of the macula flava in the human vocal fold. Part 2: its role in the growth and development of the vocal fold. Folia Phoniatr Logop 2010;62:263–70CrossRefGoogle ScholarPubMed
Sato, K. Growth and development of the human vocal fold mucosa. In: Functional Histoanatomy of the Human Larynx. Singapore: Springer, 2018: 199–211CrossRefGoogle Scholar
Sato, K. Geriatric changes of the macula flava of the human vocal fold. In: Functional Histoanatomy of the Human Larynx. Singapore: Springer, 2018:251–62CrossRefGoogle Scholar
Fawcett, DW. Mitochondria. In: The Cell. Philadelphia: W. B. Saunders, 1981:410–85Google Scholar
Sato, K, Chitose, S, Sato, K, Sato, F, Kurita, T, Umeno, H. Metabolic activity of cells in the macula flava of the human vocal fold from the aspect of mitochondrial microstructure. Laryngoscope Investig Otolaryngol 2019;4:405–9CrossRefGoogle ScholarPubMed
Kühlbrandt, W. Structure and function of mitochondrial membrane protein complexes. BMC Biol 2015;13:89CrossRefGoogle ScholarPubMed
Fawcett, DW. Mitochondria. In: A textbook of Histology. New York, Chapman & Hall; 1994:22–6Google Scholar
Pasquali-Ronchetti, I, Greenawalt, JW, Carafoli, E. On the nature of the dense matrix granules of normal mitochondria. J Cell Biol 1969;40:565–8CrossRefGoogle ScholarPubMed
Sato, K, Hirano, M, Nakashima, T. Stellate cells in the human vocal fold. Ann Otol Rhinol Laryngol 2001;110:319–25CrossRefGoogle ScholarPubMed
Sato, K, Hirano, M, Nakashima, T. Vitamin A-storing stellate cells in the human vocal fold. Acta Otolaryngol 2003;123:106–10CrossRefGoogle ScholarPubMed
Riz, I, Hawley, RG. Genomic stability in stem cells. In: Rajasekhar, VK, Vemuri, MC, eds. Regulatory Networks in Stem Cells. New York: Humana Press, 2009:67–74CrossRefGoogle Scholar
Nesti, C, Pasquali, L, Mancuso, M, Siciliano, G. The role of mitochondria in stem cell biology. In: Rajasekhar, VK, Vemuri, MC, eds. Regulatory Networks in Stem Cells. New York: Humana Press; 2009: 135–43Google Scholar
Suda, T, Takubo, K, Semenza, GL. Metabolic regulation of hematopoietic stem cells in the hypoxic niche. Cell Stem Cell 2011;9:298–310CrossRefGoogle ScholarPubMed