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Comparison of DNAzyme activity for the development of an immobilized heme sensor

Published online by Cambridge University Press:  05 January 2018

Natalie Hughes ,
Nancy Nguyen ,
Deanna-Kaye Daley ,
Justin Grennell ,
Amira Gee  and
Mehnaaz F. Ali
Natalie Hughes
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Nancy Nguyen
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Deanna-Kaye Daley
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Justin Grennell
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Amira Gee
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
Mehnaaz F. Ali*
Affiliation:
Department of Chemistry, Xavier University of Louisiana, 1, Drexel Drive, New Orleans LA70125
*
*(Email: mali2@xula.edu)
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Abstract

Point-of-care systems require highly sensitive, quantitative and selective detection platforms for the real-time multiplexed monitoring of target analytes. To ensure facile development of a sensor, it is preferable for the detection assay to have minimal chemical complexity, contain no wash steps and provide a wide and easily adaptable detection range for multiple targets. Current studies involve label-free detection strategy for relevant clinical molecules such as heme using G-quadruplex based self-assembly. We have explored the measurement of binding and kinetic parameters of various G-quadruplex/heme complexes which are able to self-associate to form a DNAzyme with peroxidase mimicking capabilities and are critical to nucleic acid research. The detection strategy includes immobilizing the G-quadruplex sequences within a polymer matrix to provide a self-assembly based detection approach for heme that could be translated towards other clinically relevant targets.

Keywords

devicesopticalsensor
Type
Articles
Information
MRS Advances , Volume 3 , Issue 26: Biomaterials and Soft Materials , 2018 , pp. 1491 - 1496
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Copyright
Copyright © Materials Research Society 2017 

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References

REFERENCES

Zhu, M., Zhang, H., Schmidt, E., and Jayawickramarajah, J., “Covalent and Non-Covalent Porphyrin-DNA conjugates,” in Handbook of Porphyrin Science. vol. 39, Kadish, K. M. S. K., Guilard, R, Ed., ed, 2016.Google Scholar
Burge, S., Parkinson, G. N., Hazel, P., Todd, A. K., and Neidle, S., “Quadruplex DNA: sequence, topology and structure,” Nucleic Acid Res., vol. 34, pp. 5402–5415, 2006.CrossRefGoogle Scholar
Cree, S. L. and Kennedy, M. A., “Relevance of G-quadruplex structures to pharmacogenetics,” Frontiers in Pharmacology, vol. 5, pp. 1–8, 2014.Google Scholar
Zhu, L., Li, C., Zhu, Z., Liu, D., Zou, Y., Wang, C., et al., “In Vitro Selection of Highly Efficient G-Quadruplex-Based DNAzymes,” Anal Chem, vol. 84, pp. 8383–8390, 2012.Google Scholar
Ambrus, A., Chen, D., Dai, J., Bialis, T., Jones, R. A., and Yang, D., “Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution,” Nucleic Acid Res., vol. 34, pp. 2723–2735, 2006.Google Scholar
Shi, Y., Huang, W. T., Luo, H. Q., and Li, N. B., “A label-free DNA reduced graphene oxide-based fluorescent sensor for highly sensitive and selective detection of hemin,” Chem Comm, vol. 47, pp. 4676–4678, 2011.Google Scholar
Pelossof, G., Tel-Vered, R., Elbaz, J., and Willner, I., “Amplified Biosensing using the Horseradish Peroxidase-Mimicking DNAzyme as an Electrocatalyst,” Anal Chem, vol. 82, pp. 4396–4402, 2010.CrossRefGoogle Scholar
Zhu, L., Li, C., Zhu, Z., Liu, D., Zou, Y., Wang, C., et al., “In Vitro Selection of Highly Efficient G-Quadruplex-Based DNAzymes,” Anal Chem, vol. 84, pp. 8383–8390, 2012.Google Scholar
Leng, Y., Sun, K., Chen, X., and Li, W., “Suspension arrays based on nanoparticle-encoded microspheres for high-throughput multiplexed detection,” Chem Soc Rev, vol. 44, pp. 5552–95, 2015.CrossRefGoogle Scholar