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Single Osteoblast Chemical Sensor via Non-invasive Bio-Electronic Interface

Published online by Cambridge University Press:  01 February 2011

Mo Yang ,
Xuan Zhang ,
Bonnie Kohr ,
Andre Morgan  and
Cengiz S Ozkan
Mo Yang
Affiliation:
Mechanical Engineering Department, University of California, Riverside, CA 92521
Xuan Zhang
Affiliation:
Mechanical Engineering Department, University of California, Riverside, CA 92521
Bonnie Kohr
Affiliation:
Mechanical Engineering Department, University of California, Riverside, CA 92521
Andre Morgan
Affiliation:
Mechanical Engineering Department, University of California, Riverside, CA 92521
Cengiz S Ozkan
Affiliation:
Mechanical Engineering Department, University of California, Riverside, CA 92521
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Abstract

The broad-spectrum sensitivity of cell based biosensors offers the capability for detecting known and unknown chemical/biological agents. One cellular parameter that is often measured is the extracellular potential of electrically active cells. Membrane excitability in osteoblasts plays a key role in modulating the electrical activity in the presence of chemical agents. However, the complexity of this signal makes interpretation of the cellular response to a chemical agent difficult to interpret. By analyzing shifts in the signal's power spectrum, it is possible to determine a frequency spectrum also known as Signature Pattern Vectors (SPV) specific to a chemical. We used a 5x5 multiple microelectrode array system to spatially position osteoblast cells, by using a gradient AC field. Fast Fourier Transformation (FFT) analyses were used to extract information pertaining to the frequency of firing from the extracellular potential.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 782: Symposium A – Micro- and Nanosystems , 2003 , A5.9
Copyright
Copyright © Materials Research Society 2004

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