Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-10-31T23:41:29.254Z Has data issue: false hasContentIssue false

RAPD marker and isozyme characterization of Sphaeropsis sapinea from diverse coniferous hosts and locations

Published online by Cambridge University Press:  01 September 1999

GLEN R. STANOSZ
Affiliation:
Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, 53706, U.S.A.
WIJNAND J. SWART
Affiliation:
Department of Plant Pathology, University of the Orange Free State, Bloemfontein 9300, Republic of South Africa
DENISE R. SMITH
Affiliation:
Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, 53706, U.S.A.
Get access

Abstract

Two distinct groups (A and B) of the conifer shoot blight and canker pathogen Sphaeropsis sapinea have been recognized on pine hosts in the northcentral United States. Their occurrence in a collection of isolates from much more diverse hosts and locations was studied by analysis of RAPD markers and isozymes. Seventy-nine isolates were used for RAPD marker analysis, and 37 for isozyme analysis, with 33 isolates in common. Hosts included 19 species in Pinus and species of Cedrus, Larix, Picea, Pseudotsuga, located in Africa, Australasia, Europe, and North America. Relationships among these isolates were determined using cluster analyses of presence or absence data for each amplification product or isozyme. Analyses of either RAPD marker or isozyme data placed most isolates in a group that contained previously characterized RAPD marker group A isolates. There was no strong indication of either host or geographic clustering within this highly similar, widely distributed group. Analysis of RAPD marker data placed several other isolates in a separate group that contained previously characterized RAPD marker group B isolates. This is the first report of isolates from hosts other than Pinus banksiana or P. resinosa, and from outside Michigan, Minnesota, or Wisconsin, that have been characterized as members of the RAPD marker group B.

Type
Research Article
Copyright
© The British Mycological Society 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)