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Direct and glia-mediated effects of GABA on development of central olfactory neurons

Published online by Cambridge University Press:  06 July 2012

Heather S. Mallory ,
Nicholas J. Gibson ,
Jon H. Hayashi ,
Alan J. Nighorn  and
Lynne A. Oland
Heather S. Mallory
Affiliation:
Department of Neuroscience, University of Arizona, Tucson, AZ, USA Department of Biological Sciences, The George Washington University, Washington, DC, USA
Nicholas J. Gibson
Affiliation:
Department of Neuroscience, University of Arizona, Tucson, AZ, USA
Jon H. Hayashi
Affiliation:
Biosciences Department, Phoenix College, Phoenix, AZ, USA
Alan J. Nighorn
Affiliation:
Department of Neuroscience, University of Arizona, Tucson, AZ, USA
Lynne A. Oland*
Affiliation:
Department of Neuroscience, University of Arizona, Tucson, AZ, USA
*
Correspondence should be addressed to: Lynne A. Oland, Department of Neuroscience, University of Arizona, PO Box 210077, Tucson, AZ 85721-0077, USA. phone: 520-621-6671, fax: 520-621-8282 email: oland@email.arizona.edu
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Abstract

Previously studied for its role in processing olfactory information in the antennal lobe, GABA also may shape development of the olfactory pathway, acting either through or on glial cells. Early in development, the dendrites of GABAergic neurons extend to the glial border that surrounds the nascent olfactory lobe neuropil. These neuropil glia express both GABAA and GABAB receptors, about half of the glia in acute cultures responded to GABA with small outward currents, and about a third responded with small transient increases in intracellular calcium. The neuronal classes that express GABA in vivo, the local interneurons and a subset of projection neurons, also do so in culture. Exposure to GABA in culture increased the size and complexity of local interneurons, but had no effect on glial morphology. The presence of glia alone did not affect neuronal morphology, but in the presence of both glia and GABA, the growth-enhancing effects of GABA on cultured antennal lobe neurons were eliminated. Contact between the glial cells and the neurons was not necessary. Operating in vivo, these antagonistic effects, one direct and one glia mediated, could help to sculpt the densely branched, tufted arbors that are characteristic of neurons innervating olfactory glomeruli.

Keywords

Glial cellsGABA currentGABA receptorsantennal lobeManduca sexta
Type
Research Article
Information
Neuron Glia Biology , Volume 7 , Issue 2-4 , May 2011 , pp. 143 - 161
Copyright
Copyright © Cambridge University Press 2012

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