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In vitro activity of essential oils against adult and immature stages of Ctenocephalides felis felis

Published online by Cambridge University Press:  06 January 2020

João Vitor Barbosa dos Santos ,
Douglas Siqueira de Almeida Chaves ,
Marco André Alves de Souza ,
Cristiano Jorge Riger ,
Monique Moraes Lambert ,
Diefrey Ribeiro Campos ,
Leandra Oliveira Moreira ,
Rosiane Conceição dos Santos Siqueira ,
Rodrigo de Paulo Osorio  and
Fabio Boylan
...Show all authors
João Vitor Barbosa dos Santos
Affiliation:
Pharmaceutical Sciences Department, Health and Biological Science Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Douglas Siqueira de Almeida Chaves
Affiliation:
Pharmaceutical Sciences Department, Health and Biological Science Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Marco André Alves de Souza
Affiliation:
Biochemistry Department, Chemistry Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Cristiano Jorge Riger
Affiliation:
Biochemistry Department, Chemistry Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Monique Moraes Lambert
Affiliation:
Animal Parasitology Department, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Diefrey Ribeiro Campos
Affiliation:
Animal Parasitology Department, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Leandra Oliveira Moreira
Affiliation:
Animal Parasitology Department, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Rosiane Conceição dos Santos Siqueira
Affiliation:
Biochemistry Department, Chemistry Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Rodrigo de Paulo Osorio
Affiliation:
Biochemistry Department, Chemistry Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
Fabio Boylan
Affiliation:
School of Pharmacy and Pharmaceutical Sciences and Trinity Biosciences Medical Institute, Trinity College Dublin, Dublin, Ireland
Thaís Ribeiro Correia
Affiliation:
School of Pharmacy and Pharmaceutical Sciences and Trinity Biosciences Medical Institute, Trinity College Dublin, Dublin, Ireland
Katherina Coumendouros
Affiliation:
School of Pharmacy and Pharmaceutical Sciences and Trinity Biosciences Medical Institute, Trinity College Dublin, Dublin, Ireland
Yara Peluso Cid*
Affiliation:
Pharmaceutical Sciences Department, Health and Biological Science Institute, Federal Rural University of Rio de Janeiro, Seropédica, RJ, Brazil
*
Author for correspondence: Yara Peluso Cid, E-mail: yarapcid@gmail.com
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Abstract

Essential oils (EOs) are considered a new class of ecological products aimed at the control of insects for industrial and domestic use; however, there still is a lack of studies involving the control of fleas. Ctenocephalides felis felis, the most observed parasite in dogs and cats, is associated with several diseases. The aim of this study was to evaluate the in vitro activity, the establishment of LC50 and toxicity of EOs from Alpinia zerumbet (Pers.) B. L. Burtt & R. M. Sm, Cinnamomum spp., Laurus nobilis L., Mentha spicata L., Ocimum gratissimum L. and Cymbopogon nardus (L.) Rendle against immature stages and adults of C. felis felis. Bioassay results suggest that the method of evaluation was able to perform a pre-screening of the activity of several EOs, including the discriminatory evaluation of flea stages by their LC50. Ocimum gratissimum EO was the most effective in the in vitro assays against all flea stages, presenting adulticide (LC50 = 5.85 μg cm−2), ovicidal (LC50 = 1.79 μg cm−2) and larvicidal (LC50 = 1.21 μg cm−2) mortality at low doses. It also presented an excellent profile in a toxicological eukaryotic model. These findings may support studies involving the development of non-toxic products for the control of fleas in dogs and cats.

Keywords

Essential oilsfleasmortalitypets
Type
Research Article
Information
Parasitology , Volume 147 , Issue 3 , March 2020 , pp. 340 - 347
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Copyright
Copyright © Cambridge University Press 2020

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References

Abbott, WS (1987) A method of computing the effectiveness of an insecticide. Journal of the American Mosquito Control Association 3, 302–303.Google ScholarPubMed
Adams, RP (1995) Identification of Essential Oil Components by Gas Chromatography-Mass Spectroscopy. USA: Corp., Carol Stream.Google Scholar
Aggarwal, KK, Tripathi, AK, Prajapati, V and Kumar, S (2001) Toxicity of 1,8-cineole towards three species of stored product coleopterans. Insect Science and Its Application 21, 155–160.Google Scholar
Ajayi, OE, Arthur, GA and Henry, YF (2014) Fumigation toxicity of essential oil monoterpenes to Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae). Journal of Insects 2014, 1–7. doi.org/10.1155/2014/917212.CrossRefGoogle Scholar
Armijos, C, Valarezo, E, Cartuche, L, Zaragoza, T, Finzi, PV, Mellerio, GG and Vidari, G (2018) Chemical composition and antimicrobial activity of Myrcianthesfragrans essential oil, a natural aromatizer of the traditional Ecuadorian beverage colada morada. Journal of Ethnopharmacol 225, 319–326.CrossRefGoogle ScholarPubMed
Avelar, DM, Melo, MN and Linardi, PM (2011) Morphology and growth characteristics of cultured Leptomonas ctenocephali from Ctenocephalides felis felis (Siphonaptera: Pulicidae) of dogs in Brazil. Veterinary Parasitology 80, 394–398.CrossRefGoogle Scholar
Bakkali, F, Averbeck, S, Averbeck, D and Idaomar, M (2008) Biological effects of essential oils – a review. Food and Chemical Toxicology 46, 446–475.CrossRefGoogle ScholarPubMed
Batista, LCSO, Cid, YP, Almeida, AP, Prudêncio, ER, Riger, CJ, Souza, MAA, Coumendouros, K and Chaves, DSA (2016) In vitro efficacy of essential oils and extracts of Schinus molle L. against Ctenocephalides felis felis. Parasitology 143, 627–638.CrossRefGoogle Scholar
Benelli, G and Pavella, R (2018) Beyond mosquitoes – essential oil toxicity and repellency against bloodsucking insects. Industrial Crops & Products 117, 382–392.CrossRefGoogle Scholar
Bissinger, BW and Roe, RM (2010) Tick repellents: past, present, and future. Pesticide Biochemistry and Physiology 96, 63–79.CrossRefGoogle Scholar
Blagburn, BL and Dryden, MW (2009) Biology, treatment, and control of flea and tick infestations. Veterinary Clinics of North America: Small Animal Practice 39, 1173–1200.CrossRefGoogle ScholarPubMed
Carlotti, DN and Jacobs, DE (2000) Therapy, control and prevention of flea allergy dermatitis in dogs and cats. Veterinary Dermatology 11, 83–98.CrossRefGoogle Scholar
Cavalcanti, A, de Alves, MS, da Silva, LCP, dos Patrocínio, DS, Mirza Nalesso Sanches, MN, de Chaves, DSA and de Souza, MAA (2015) Volatiles composition and extraction kinetics from Schinus terebinthifolius and Schinus molle leaves and fruit. Brazilian Journal of Pharmacognosy 25, 356–362.CrossRefGoogle Scholar
Chimnoi, N, Reuk-Ngam, N, Chuysinuan, P, Khlaychan, P, Khunnawutmanotham, N, Chokchaichamnankit, D, Thamniyom, W, Klayraung, S, Mahidol, C and Techasakul, S (2018) Characterization of essential oil from Ocimum gratissimum leaves: antibacterial and mode of action against selected gastroenteritis pathogens. Microbiology Pathogens 118, 290–300.CrossRefGoogle ScholarPubMed
de Almeida, AP, Chaves, DSA, Coumendouros, K, Batista, LC de SO, Rosado, LHG, de Souza, MAA and Cid, YP (2016) Composições farmacêuticas para o tratamento e profilaxia das infestações causadas por ectoparasitas contendo óleo essencial de Schinus molle L. Titular Universidade Federal Rural do Rio de Janeiro BR 10 2016 028710 3. Depósito: 07 dez. 2016.Google Scholar
de Sá, RA, de Castro, FA, Eleutherio, EC, de Souza, RM, da Silva, JF and Pereira, MD (2013) Brazilian Propolis protects Saccharomyces cerevisiae cells against oxidative stress. Brazilian Journal of Microbiology 44, 993–1000.Google ScholarPubMed
Dryden, MW (1993) Biology of fleas of dogs and cats. Compendium of Continuing Education for the Practising Veterinarian 15, 569–579.Google Scholar
Ellse, L and Wall, R (2014) The use of essential oils in veterinary ectoparasite control: a review. Medical and Veterinary Entomology 28, 233–243.CrossRefGoogle ScholarPubMed
ESCCAP (2015) European Scientific Counsel Companion Animal Parasites, Control of Ectoparasites in Dogs and Cats, guidelines 03, 4th Edn. Malvern, Worcestershire, England: ESCCAP.Google Scholar
Fikry, S, Khalil, N and Salama, O (2019) Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare L. essential oil. AMB Express 9, 41.CrossRefGoogle ScholarPubMed
Halos, L, Beugnet, F, Cardoso, L, Farkas, R, Franc, M, Guillot, J, Pfister, K and Wall, R (2014) Flea control failure? Myths and realities. Trends in Parasitology 30, 228–233.CrossRefGoogle ScholarPubMed
Hink, WF, Liberati, TA and Collart, MG (1998) Toxicity of linalool to life stages of the cat flea, Ctenocephalides felis (Siphonaptera: Pulicidae), and its efficacy in carpet and on animals. Journal of Medical Entomology 25, 1–4.CrossRefGoogle Scholar
Huang, Y, Shuit-Hung Ho, SH, Lee, HC and Yap, YL (2002) Insecticidal properties of eugenol, isoeugenol and methyleugenol and their effects on nutrition of Sitophilus zeamais Motsch (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Journal of Stored Products Research 38, 403–412.CrossRefGoogle Scholar
Iwamatsu, T, Miyamoto, D, Mitsuno, H, Yoshioka, Y, Fujii, T, Sakurai, T, Ishikawa, Y and Kanzaki, R (2016) Identification of repellent odorants to the body louse, Pediculus Humanus corporis, in clove essential oil. Parasitology Research 115, 1659–1666.CrossRefGoogle ScholarPubMed
Jeyaratnama, N, Noura, AH, Kanthasamya, R, Nourb, AH, Yuvaraj, AR and Akindoyo, JO (2016) Essential oil from Cinnamomum cassia bark through hydrodistillation and advanced microwave assisted hydrodistillation. Industrial Crops and Products 92, 57–66.CrossRefGoogle Scholar
Koc, S, Oz, E, Aydin, L and Cetin, H (2012) Acaricidal activity of the essential oils from three Lamiaceae plant species on Rhipicephalus Turanicus Pom. (Acari: Ixodidae). Parasitology Research 111, 1863–1865.CrossRefGoogle Scholar
Lans, C, Turner, N and Khan, T (2008) Medicinal plant treatments for fleas and ear problems of cats and dogs in British Columbia, Canada. Parasitology Research 103, 889–898.CrossRefGoogle ScholarPubMed
Lehane, MJ (2005) The Biology of Blood-Sucking in Insects, Second Edition. UK: Cambridge University Press.CrossRefGoogle Scholar
Linardi, PM and Santos, JLC (2012) Ctenocephalides felis felis vs. Ctenocephalides canis (Siphonaptera: Pulicidae): some issues in correctly identify these species. Revista Brasileira de Parasitologia Veterinária 21, 345–354.CrossRefGoogle ScholarPubMed
Macchioni, F, Perrucci, S, Cioni, P, Morelli, L, Castilho, P and Cecchi, F (2006) Composition and acaricidal activity of Laurus novocanariensis and Laurus nobilis essential oils against Psoroptes cuniculi. Journal of Essential Oil Research 18, 111–114.CrossRefGoogle Scholar
Magi, E, Jarvis, T and Miller, I (2006) Effects of different plant products against pig mange mites. Acta Veterinaria Brno 75, 283–287.CrossRefGoogle Scholar
Marchese, A, Orhan, IE, Daglia, M, Barbieri, R, Di Lorenzo, A, Nabavi, SF, Gortzi, O, Izadi, M and Nabavi, SM (2016) Antibacterial and antifungal activities of thymol: a brief review of the literature. Food Chemistry 210, 402–414.CrossRefGoogle ScholarPubMed
Merghni, A, Marzouki, H, Hentati, H, Aouni, M and Mastouri, M (2015) Antibacterial and antibiofilm activities of Laurus nobilis L. essential oil against Staphylococcus aureus strains associated with oral infections. Pathology Biology (Paris) S0369–8114, 101–107.Google Scholar
Morcia, C, Tumino, G, Ghizzoni, R and Terzi, V (2016) Carvone (Mentha spicata L.) oil. In Essential Oils in Food Preservation, Flavor and Safety. Amsterdam, The Netherlands: Elsevier, pp. 309–316.CrossRefGoogle Scholar
Morey, RA and Khandagle, AJ (2012) Bioefficacy of essential oils of medicinal plants against housefly, Musca domestica L. Parasitology Research 111, 1799–1805.CrossRefGoogle ScholarPubMed
Na, YE, Kim, SI, Bang, HS, Kim, BS and Ahn, YJ (2011) Fumigant toxicity of cassia and cinnamon oils and cinnamaldehyde and structurally related compounds to Dermanyssusgallinae (Acari:Dermanyssidae). Veterinary Parasitology 178, 324–329.CrossRefGoogle Scholar
Ojimelukwe, PC and Adler, C (2000) Toxicity and repellent effects of eugenol, thymol, linalool, menthol and other pure compounds on Dinoderus bifloveatus (Coleoptera: Bostrichidae). Journal of Sustainable Agriculture and Environment 2, 47–54.Google Scholar
Pinto, NV, Assreuy, AM, Coelho-de-Souza, AN, Ceccato, VM, Magalhães, PJ, Lahlou, S and Leal-Cardiso, JH (2009) Endothelium-dependent vasorelaxant effects of the essential oil from aerial parts of Alpinia zerumbet and its main constituent 1,8-cineole in rats. Phytomedicine 16, 1151–1155.CrossRefGoogle ScholarPubMed
Rust, M and Dryden, M (1997) The biology, ecology and management of the cat flea. Annual Review of Entomology 42, 451–473.CrossRefGoogle ScholarPubMed
Sadaria, AM, Sutton, R, Moran, KD, Teerlink, J, Brown, JV and Halden, RU (2017) Passage of fiproles and imidacloprid from urban pest control uses through wastewater treatment plants in northern California, USA. Environmental and Toxicology Chemistry 36, 1473–1482.CrossRefGoogle ScholarPubMed
Sfara, V, Zerba, EN and Alzogaray, RA (2009) Fumigant insecticidal activity and repellent effect of five essential oils and seven monoterpenes on first-instar nymphs of Rhodnius prolixus. Journal of Medical Entomology 46, 511–515.CrossRefGoogle ScholarPubMed
Teerlink, J, Hernandez, J and Budd, R (2017) Fipronil washoff to municipal wastewater from dogs treated with spot-on products. Science Total Environmental 599–600, 960–966.CrossRefGoogle ScholarPubMed
Toloza, AC, Zygadlo, J, Mougabure Cueto, G, Biurrun, F, Zerba, E and Picollo, MI (2006) Fumigant and repellent properties of essential oils and component compounds against permethrin-resistant Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina. Journal of Medical Entomology 43, 889–895.CrossRefGoogle ScholarPubMed
Tripathi, A and Mishra, AK (2017) Knowledge and passive adaptation to climate change: an example from Indian farmers. Climate Risk Management 16, 195–207.CrossRefGoogle Scholar
Van den Dool, H and Kratz, PD (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography 11, 463–471.CrossRefGoogle Scholar
Weng, DCJ, Latip, J, Hasbullah, SA and Sastrohamidjojo, H (2015) Optimal extraction and evaluation on the oil content of citronella oil extracted from Cymbopogon nardus. Malaysian Journal of Analytical Sciences 19, 71–76.Google Scholar
Yones, DA, Bakir, HY and Bayoumi, SAL (2016) Chemical composition and efficacy of some selected plant oils against Pediculus humanus capitis in vitro. Parasitology Research 115, 3209–3218.CrossRefGoogle ScholarPubMed
Zaridah, MZ, Nor Azah, MA, Abu Said, A and Mohd Faridz, Z (2003) Larvicidal properties of citronellal and Cymbopogon nardus essential oils from two different localities. Tropical Biomedicine 20, 169–174.Google Scholar
Zhang, L, Yang, Z, Chen, D, Huang, Z, Li, Y, Lan, X, Su, P, Pan, W, Zhou, W, Zheng, X and Du, Z (2017) Variation on composition and bioactivity of essential oils of four common curcuma herbs. Chemistry & Biodiversity 14, 11.CrossRefGoogle ScholarPubMed