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Prevalence and phylogeography of Taenia hydatigena metacestodes from goats of India

Published online by Cambridge University Press:  16 May 2022

Aman D. Moudgil Open the ORCID record for Aman D. Moudgil [Opens in a new window] ,
Anil K. Nehra ,
Sukhdeep Vohra ,
Sidharath Dev Thakur  and
Dinesh Sharma
Aman D. Moudgil*
Affiliation:
Department of Veterinary Parasitology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004, India
Anil K. Nehra
Affiliation:
Department of Veterinary Parasitology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004, India
Sukhdeep Vohra
Affiliation:
Department of Veterinary Parasitology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana 125004, India
Sidharath Dev Thakur
Affiliation:
Department of Veterinary Public Health and Epidemiology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, H.P. 176062, India
Dinesh Sharma
Affiliation:
Department of Veterinary Pharmacology, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishwavidyalaya, Palampur, H.P. 176062, India
*
Author for correspondence: Aman D. Moudgil, E-mail: Moudgil.aman@gmail.com
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Abstract

The study determined the prevalence and genetic population structure relationships of Cysticercus tenuicollis (Taenia hydatigena metacestode) retrieved from the goats slaughtered in north India. An overall prevalence of 9.62% (59/613) was recorded. Genetic population structure relationships were assessed by targeting partial cytochrome c oxidase subunit 1 mitochondrial gene sequence. Phylogenetic tree analysis revealed that all the present study representative isolates (n = 7) formed a major clade and grouped with T. hydatigena isolates retrieved from sheep, goats, pigs and dogs, originating from China, Iran, Nigeria, Ghana and Poland. However, a single isolate from Himachal Pradesh (isolate 3) formed a subgroup within the clade. The neutrality and diversity indices revealed high values of haplotype diversity [Hd = 0.99695 (0.95238–1.0000)] and low nucleotide diversity (π = 0.49276), which was indicative of demographic expansion and low gene flow, suggesting that Indian T. hydatigena isolates were not genetically differentiated. Tajima's D (−1.26988) and Fu and Li's D statistics values (−0.74556) were negative, demonstrating deviations from neutrality and both propounded recent population expansion or purifying selection. Results highlighted a low genetic diversity of T. hydatigena metacestodes across the geographical range of north India.

Keywords

Cysticercus tenuicolliscytochrome c oxidase subunit 1 genegenetic variationphylogeography
Type
Research Article
Information
Parasitology , Volume 149 , Issue 9 , August 2022 , pp. 1193 - 1198
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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References

Abbas, I, El-Alfy, ES, Janecek-Erfurth, E and Strube, C (2021) Molecular characterization of Cysticercus tenuicollis isolates from sheep in the Nile Delta, Egypt and a review on Taenia hydatigena infections worldwide. Parasitology 148, 913–933.CrossRefGoogle Scholar
Adwan, K, Jayousi, A, Abuseir, S, Abbasi, I, Adwan, G and Jarrar, N (2018) Genetic diversity of Taenia hydatigena in the northern part of the West Bank, Palestine as determined by mitochondrial DNA sequences. Acta Parasitologica 63, 299–303.CrossRefGoogle ScholarPubMed
Bandelt, H, Forster, P and Röhl, A (1999) Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution 16, 37–48.CrossRefGoogle ScholarPubMed
Boufana, B, Scala, A, Lahmar, S, Pointing, S, Craig, PS, Dessì, G, Zidda, A, Pipia, AP and Varcasia, A (2015) A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy. Veterinary Parasitology 214, 67–74.CrossRefGoogle ScholarPubMed
Bowles, J, Blair, D and McManus, DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165–173.CrossRefGoogle ScholarPubMed
Braae, UC, Kabululu, M, Nørmark, ME, Nejsum, P, Ngowi, HA and Johansen, MV (2015) Taenia hydatigena cysticercosis in slaughtered pigs, goats, and sheep in Tanzania. Tropical Animal Health and Production 47, 1523–1530.CrossRefGoogle ScholarPubMed
Celik, F, Kilinc, SG, Kesik, HK, Ahemd, H and Simsek, S (2021) In-silico analysis of mt-CO1 gene of Taenia hydatigena sheep isolates. Helminthologia 58, 59–67.CrossRefGoogle ScholarPubMed
Cengiz, G, Yucel Tenekeci, G and Bilgen, N (2019) Molecular and morphological characterization of Cysticercus tenuicollis in red deer (Cervus elaphus) from Turkey. Acta Parasitologica 64, 652–657.CrossRefGoogle Scholar
Chakraborty, M and Gupta, D (2017) Small ruminant value chain in India: opportunities and constraints. Indian Journal of Economics and Development 5, 1–5.Google Scholar
Filip, KJ, Pyziel, AM, Jeżewski, W, Myczka, AW, Demiaszkiewicz, AW and Laskowski, Z (2019) First molecular identification of Taenia hydatigena in wild ungulates in Poland. Ecohealth 16, 161–170.CrossRefGoogle ScholarPubMed
Fu, YX and Li, WH (1993) Statistical tests of neutrality of mutations. Genetics 133, 693–709.CrossRefGoogle ScholarPubMed
Hall, TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Kedra, AH, Tkach, VV, Swiderski, Z and Pawłowski, Z (2001) Intraspecific variability among NADH dehydrogenase subunit 1 sequences of Taenia hydatigena. Parasitology International 50, 145–148.CrossRefGoogle ScholarPubMed
Kilinc, SG, Kesik, HK and Simsek, S (2019) Molecular characterization and haplotypes of sheep and goat isolates of Cysticercus tenuicollis in Turkey. Parasitology 146, 1047–1054.CrossRefGoogle ScholarPubMed
Kumar, S, Stecher, G, Li, M, Knyaz, C and Tamura, K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35, 1547–1549.CrossRefGoogle ScholarPubMed
Loos-Frank, B (2000) An up-date of Verster's (1969) ‘taxonomic revision of the genus Taenia Linnaeus’ (Cestoda) in table format. Systematic Parasitology 45, 155–183.CrossRefGoogle Scholar
Moudgil, AD, Moudgil, P, Asrani, RK and Agnihotri, RK (2019) Hydatidosis in slaughtered sheep and goats in India: prevalence, genotypic characterization and pathological studies. Journal of Helminthology 94, e27.CrossRefGoogle ScholarPubMed
Moudgil, AD, Sharma, S, Sharma, D, Sharma, K and Agnihotri, RK (2020) Concurrent ogmocotylosis and cysticercosis in hilly migratory sheep flock. Indian Journal of Small Ruminants 26, 135–137.CrossRefGoogle Scholar
Moudgil, AD, Nehra, AK, Nehra, V, Sharma, R, Vohra, S and Moudgil, P (2021) Phylogenetics and pathology of hydatid disease in slaughtered buffaloes of North India. Acta Parasitologica 66, 899–906.CrossRefGoogle ScholarPubMed
Nath, S, Pal, S, Mandal, S and Parveen, K (2010) Prevalence of caprine Taenia hydatigena cysticercosis (Cysticercus tenuicollis) in Durg, Chhattisgarh. Indian Journal of Field Veterinarians 5, 64–66.Google Scholar
Nimbalkar, RK, Shinde, S, Kamtikar, VN and Muley, SP (2011) Study on Taenia hydatigena in slaughtered sheep (Ovis aries) and goats (Capra hircus) in Maharashtra, India. Global Veterinaria 6, 374–377.Google Scholar
Ohiolei, JA, Luka, J, Zhu, GQ, Yan, HB, Li, L, Magaji, AA, Alvi, MA, Wu, YT, Li, JQ, Fu, BQ and Jia, WZ (2019) First molecular description, phylogeny and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes. Parasites and Vectors 12, 520.CrossRefGoogle ScholarPubMed
Ohiolei, J, Yan, H, Li, L, Li, W, Wu, Y, Alvi, M, Zhang, N-Z, Fu, BQ, Wang, X-L and Jia, W (2021) A new molecular nomenclature for Taenia hydatigena: mitochondrial DNA sequences reveal sufficient diversity suggesting the assignment of major haplotype divisions. Parasitology 148, 311–326.CrossRefGoogle ScholarPubMed
Omar, MA, Elmajdoub, LO, Al-Aboody, MS, Elsify, AM, Elkhtam, AO and Hussien, AA (2016) Molecular characterization of Cysticercus tenuicollis of slaughtered livestock in Upper Egypt governorates. Asian Pacific Journal of Tropical Biomedicine 6, 706–708.CrossRefGoogle Scholar
Pathak, KM and Gaur, SN (1982) The incidence of adult and larval stage Taenia hydatigena in Uttar Pradesh (India). Veterinary Parasitology 10, 91–95.CrossRefGoogle Scholar
Pathak, KM, Gaur, SN and Sharma, SN (1982) The pathology of Cysticercus tenuicollis infection in goats. Veterinary Parasitology 11, 131–139.CrossRefGoogle ScholarPubMed
Pathak, KM, Kumar, M and Gaur, SN (1984) Changes in blood cellular components, serum proteins and serum enzyme activities in pigs naturally infected with Cysticercus tenuicollis. Research in Veterinary Science 36, 263–265.CrossRefGoogle ScholarPubMed
Rostami, S, Salavati, R, Beech, RN, Babaei, Z, Sharbatkhori, M, Baneshi, MR, Hajialilo, E, Shad, H and Harandi, MF (2015) Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran. Journal of Helminthology 89, 150–157.CrossRefGoogle ScholarPubMed
Sarvi, S, Ebrahimi Behrestaghi, L, Alizadeh, A, Abdollah Hosseini, S, Gohardieh, S, Bastani, R, Yazdani Charati, J, Daryani, A, Amouei, A, Spotin, A and Gholami, S (2020) Morphometric, genetic diversity and phylogenetic analysis of Taenia hydatigena (Pallas, 1766) larval stage in Iranian livestock. Parasitology 147, 231–239.CrossRefGoogle ScholarPubMed
Scala, A, Urrai, G, Varcasia, A, Nicolussi, P, Mulas, M, Goddi, L, Pipia, AP, Sanna, G, Genchi, M and Bandino, E (2016) Acute visceral cysticercosis by Taenia hydatigena in lambs and treatment with praziquantel. Journal of Helminthology 90, 113–116.CrossRefGoogle ScholarPubMed
Sgroi, G, Varcasia, A, Dessì, G, D'Alessio, N, Pacifico, L, Buono, F, Neola, B, Fusco, G, Santoro, M, Toscano, V, Fioretti, A and Veneziano, V (2019) Massive Taenia hydatigena cysticercosis in a wild boar (Sus scrofa) from Italy. Acta Parasitologica 64, 938–941.CrossRefGoogle Scholar
Singh, BB, Sharma, JK, Ghatak, S, Sharma, R, Bal, MS, Tuli, A and Gill, JP (2012) Molecular epidemiology of echinococcosis from food producing animals in north India. Veterinary Parasitology 186, 503–506.CrossRefGoogle ScholarPubMed
Singh, BB, Sharma, R, Gill, JP and Sharma, JK (2015) Prevalence and morphological characterisation of Cysticercus tenuicollis (Taenia hydatigena cysts) in sheep and goat from north India. Journal of Parasitic Diseases 39, 80–84.CrossRefGoogle ScholarPubMed
Tajima, F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123, 585–595.CrossRefGoogle ScholarPubMed
Thrusfield, M (2007) Veterinary Epidemiology, 3rd Edn. Oxford, UK: Blackwell Science Ltd.Google Scholar