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Therapeutic efficacy of mebendazole and artemisinin in different phases of trichinellosis: a comparative experimental study

Published online by Cambridge University Press:  13 January 2021

Amal Farahat Allam Open the ORCID record for Amal Farahat Allam [Opens in a new window] ,
Reham Ahmed Mostafa ,
Wael Lotfy ,
Hoda Fahmy Farag ,
Naglaa Fathi ,
Esraa Abdelhamid Moneer  and
Amel Youssef Shehab
Amal Farahat Allam*
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
Reham Ahmed Mostafa
Affiliation:
Department of chemistry, Faculty of Education, Alexandria University, Alexandria, Egypt
Wael Lotfy
Affiliation:
Faculty of Nursing, Matrouh University, Matrouh, Egypt
Hoda Fahmy Farag
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
Naglaa Fathi
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
Esraa Abdelhamid Moneer
Affiliation:
Department of Medical Laboratory, Faculty of Allied Medical Sciences, Pharos University, Alexandria, Egypt
Amel Youssef Shehab
Affiliation:
Medical Research Institute, University of Alexandria, Department, Alexandria, Egypt
*
Author for correspondence: Amal Farahat Allam, E-mail: amalalam2005@yahoo.com
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Abstract

The present work aimed at studying the efficacy of mebendazole (MBZ) compared to artemisinin (ART) for the treatment of trichinellosis at various phases of infection. Seventy Swiss albino mice were orally infected by 300 Trichinella spiralis (T. spiralis) larvae. Mice were divided into infected untreated control group and infected groups treated with 50 mg kg−1 MBZ and 300 mg kg−1 ART for three and five consecutive days, respectively, at the enteral phase [2–4 days post infection (PI)], invasive phase (10–12 days PI) and encapsulated phase (28–30 days PI). All mice were sacrificed 35–42 days PI. MBZ and ART revealed a significant decrease in mean larval counts and increase of larval per cent reduction (LR %) when treatment was initiated during the enteral phase compared to the other phases. MBZ showed significantly higher LR % (99.7, 83.95 and 89.65%) than ART (80.58, 67.0 and 79.2%) when administered at the three infection phases. Histopathological study showed a decrease in the number of encysted larvae, their surrounding cellular infiltrates and increased regenerative muscles in all treated mice. In conclusion, ART possesses a substantial anthelmintic activity against T. spiralis infection in mice both at the enteral and encapsulated phases, yet, significantly lower than MBZ.

Keywords

Artemisininencysted larvaemebendazolemiceTrichinella spiralis
Type
Research Article
Information
Parasitology , Volume 148 , Issue 5 , April 2021 , pp. 630 - 635
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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References

Abou Rayia, DM, Saad, AE, Ashour, DS and Oreiby, RM (2017) Implication of artemisinin nematocidal activity on experimental trichinellosis: in vitro and in vivo studies. International Journal for Parasitology 66, 56–63.CrossRefGoogle ScholarPubMed
Basyoni, MM and El-Sabaa, AA (2013) Therapeutic potential of myrrh and ivermectin against experimental Trichinella spiralis infection in mice. Korean Journal of Parasitology 51, 297–304.CrossRefGoogle ScholarPubMed
Bruschi, F and Murrell, KD (2002) New aspects of human trichinellosis: the impact of new Trichinella species. Postgraduate Medical Journal 78, 15–22.CrossRefGoogle ScholarPubMed
Caner, A, Döşkaya, M, Değirmenci, A, Can, H, Baykan, Ş, Üner, A, Başdemir, G, Zeybek, U and Gürüz, Y (2008) Comparison of the effects of Artemisia vulgaris and Artemisia absinthium growing in western Anatolia against trichinellosis (Trichinella spiralis) in rats. Experimental Parasitology 119, 173–179.CrossRefGoogle ScholarPubMed
Carleton, MA, Drury, GA, Willington, EA and Cammeron, H (1967) Carleton's Histological Technique, 4th Edn., New York, Toronto, London: Oxford Univ. Press.Google Scholar
Codina, AV, García, A, Leonardi, D, Vasconi, MD, Di Masso, RJ, Lamas, MC and Hinrichsen, LI (2015) Efficacy of albendazole: β-cyclodextrin citrate in the parenteral stage of Trichinella spiralis infection. International Journal of Biological Macromolecules 77, 203–206.CrossRefGoogle ScholarPubMed
Cui, L and Su, XZ (2009) Discovery, mechanisms of action and combination therapy of artemisinin. Expert Review of Anti-infective Therapy 7, 999–1013.CrossRefGoogle ScholarPubMed
De la Rosa, JL, Alvarez, N and Gómez-Priego, A (2008) Study of the reproductive capacity of Trichinella spiralis recovered from experimentally infected mice under-dosed with albendazole or mebendazole. Tropical Biomedicine 24, 93–97.Google Scholar
De la Torre-Iglesias, PM, García-Rodriguez, JJ, Torrado, G, Torrado, S, Torrado-Santiago, S and Bolás-Fernández, F (2014) Enhanced bioavailability and anthelmintic efficacy of mebendazole in redispersible microparticles with low-substituted hydroxypropylcellulose. Drug Design, Development and Therapy 18, 1467–1479.Google Scholar
Dupouy-Camet, J (2000) Trichinellosis: a worldwide zoonosis. Veterinary Parasitology 93, 191–200.CrossRefGoogle ScholarPubMed
Dyab, AK, Ahmed, MA and Abdelazeem, AG (2019) Prevalence and histopathology of Trichinella spiralis larvae of slaughtered pigs in Cairo governorate, Egypt. Journal of the Egyptian Society of Parasitology 49, 439–442.CrossRefGoogle Scholar
Gottstein, B, Pozio, E and Nöckler, K (2009) Epidemiology, diagnosis, treatment, and control of trichinellosis. Clinical Microbiology Reviews 22, 127–145.CrossRefGoogle ScholarPubMed
Held, J, Soomro, SA, Kremsner, PG, Jansen, FH and Mordmüller, B (2011) In vitro activity of new Artemisinin derivatives against Plasmodium falciparum clinical isolates from Gabon. International Journal of Antimicrobial Agents 37, 485–488.CrossRefGoogle ScholarPubMed
Hong, ST (2018) Albendazole and praziquantel: review and safety monitoring in Korea. Infection and Chemotherapy 50, 1–10.CrossRefGoogle ScholarPubMed
Kapel, CM, Webster, P and Gamble, HR (2005) Muscle distribution of sylvatic and domestic Trichinella larvae in production animals and wildlife. Veterinary Parasitology 132, 101–105.CrossRefGoogle ScholarPubMed
Keiser, J and Utzinger, J (2007) Food-borne trematodiasis: current chemotherapy and advances with artemisinins and synthetic trioxolanes. Trends in Parasitology 23, 555–562.CrossRefGoogle ScholarPubMed
Keittivuti, A and Keittivuti, B (1989) Anthelmintic effects of albendazole, mebendazole and diethylcarbamazine on Trichinella spiralis in mice. Journal of the Science Society of Thailand 15, 49–54.CrossRefGoogle Scholar
Li, HJ, Wang, W, Li, YZ, Qu, GL, Xing, YT, Tao, YH, Wei, JY, Dai, JR and Liang, YS (2011) Effects of artemether, artesunate and dihydroartemisinin administered orally at multiple doses or combination in treatment of mice infected with Schistosoma japonicum. Parasitology Research 109, 515–519.CrossRefGoogle ScholarPubMed
Lopez-Garcia, ML, Torrado-Duran, S, Torrado-Duran, J, Martínez-Fernández, AR and Bolás-Fernández, F (1997) Albendazole versus ricobendazole (albendazole-sulphoxide) against enteral and parenteral stages of Trichinella spiralis in mice. International Journal for Parasitology 27, 781–785.CrossRefGoogle ScholarPubMed
McCracken, RO and Taylor, DD (1980) Mebendazole therapy of parenteral trichinellosis. Science 207, 1220–1222.CrossRefGoogle ScholarPubMed
Murrell, KD and Pozio, E (2011) Worldwide occurrence and impact of human trichinellosis, 1986-2009. Emerging Infectious Diseases 17, 2194–2202.CrossRefGoogle ScholarPubMed
Nair, AB and Jacob, S (2016) A simple practice guide for dose conversion between animals and human. Journal of Basic and Clinical Pharmacy 7, 27–31.CrossRefGoogle ScholarPubMed
Obistioiu, D, Cristina, RT, Schmerold, I, Chizzola, R, Stolze, K, Nichita, I and Chiurciu, V (2014) Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris. Chemistry Central Journal 8, 6.CrossRefGoogle ScholarPubMed
Pozio, E, Sacchini, D, Sacchi, L, Tamburrini, A and Alberici, F (2001) Failure of mebendazole in the treatment of humans with Trichinella spiralis infection at the stage of encapsulating larvae. Clinical Infectious Diseases 32, 638–642.CrossRefGoogle Scholar
Rainova, I, Kaftandjiev, I, Harizanov, R, Tsvetkova, N, Jordanova, D, Marinova, I, Kurdova, R, Kantardjiev, T and Lalkovski, N (2016) Outbreaks of human trichinellosis, still a challenge for the public health authorities in Bulgaria. Journal of Public Health 24, 291–297.CrossRefGoogle Scholar
Rodríguez, JJG, de Prada, I, Durán, JJT and Fernández, FB (2009) The effect of intestinal trichinellosis on oral bioavailability of albendazole in mice. Parasitology Research 105, 65–70.CrossRefGoogle ScholarPubMed
Soliman, GA, Taher, ES and Mahmoud, MA (2011) Therapeutic efficacy of Dormectin, ivermectin and levamisole against different stages of Trichinella spiralis in rats. Turkiye Parazitol Derg 35, 86–91.CrossRefGoogle ScholarPubMed
Sukul, NC, Ghosh, S and Sinhababu, SP (2005) Reduction in the number of infective Trichinella spiralis larvae in mice by use of homeopathic drugs. Research in Complementary Medicine 12, 202–205.CrossRefGoogle ScholarPubMed
Sun, S, Li, H, Yuan, Y, Wang, L, He, W and Xie, H (2019) Preventive and therapeutic effects of Trichinella spiralis adult extracts on allergic inflammation in an experimental asthma mouse model. Parasites & Vectors 12, 326.CrossRefGoogle Scholar
Tolstoj, VA, Lytvynets, A and Langrova, I (2007) Pro-oxidant effects of mebendazole in albino rats experimentally infected with Trichinella spiralis. Parasitology Research 100, 1277–1280.CrossRefGoogle ScholarPubMed
Vadlamudi, HC, Reddy, D and Raju, P (2015) A critical analysis on the bioavailability enhancement approaches for mebendazole. JGTPS 6, 2528–2533.Google Scholar
Velebny, S, Tomasovicova, O and Stpiczynska, R (1992) Pharmacokinetics of 3H-cambendazole in mice in the course of experimental trichinellosis. Helminthologia 29, 207–210.Google Scholar