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Embryo manipulation in neotropical characiform fish: incubation system, anaesthetic, and PGC transplantation in Prochilodus lineatus

Published online by Cambridge University Press:  05 August 2022

Gabriella Braga Carvalho Open the ORCID record for Gabriella Braga Carvalho [Opens in a new window] ,
Geovanna Carla Zacheo Coelho ,
Andreoli Correia Alves Open the ORCID record for Andreoli Correia Alves [Opens in a new window] ,
Amanda Pereira Dos Santos Silva ,
Paulo Sérgio Monzani ,
José Augusto Senhorini Open the ORCID record for José Augusto Senhorini [Opens in a new window] ,
Norberto Castro Vianna  and
George Shigueki Yasui
Gabriella Braga Carvalho*
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
Geovanna Carla Zacheo Coelho
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
Andreoli Correia Alves
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
Amanda Pereira Dos Santos Silva
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
Paulo Sérgio Monzani
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil Institute of Bioscience, São Paulo State University, Botucatu, Brazil
José Augusto Senhorini
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil Institute of Bioscience, São Paulo State University, Botucatu, Brazil
Norberto Castro Vianna
Affiliation:
China Three Gorges Corporation Brazil, Chavantes, Brazil
George Shigueki Yasui
Affiliation:
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil Institute of Bioscience, São Paulo State University, Botucatu, Brazil
*
Author for correspondence: Gabriella Braga Carvalho. Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária São Paulo, SP, Brazil. CEP: 05508–270. E-mail: gabriellacarvalho@usp.br
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Summary

Primordial germ cells transplantation is a unique approach for conservation and reconstitution of endangered fish species. This study aimed to establish techniques to culture dechorionated embryos in different incubation systems and also to determine anaesthetic concentration for fish recipients in the larval stage for subsequent primordial germ cell transplantation. Intact and dechorionated embryos were divided into three incubation systems: (1) a control group with manual replacement of the solution; (2) a closed environment with high oxygen with manual replacement of the solution; and (3) constant solution recirculation. This combination resulted in six treatments. For the evaluation of anaesthetics for larvae, the concentrations evaluated were 19.5 mM, 24.4 mM, 29.3 mM, and 34.2 mM of 2-phenoxyethanol. Anaesthesia concentration and recovery at different stages were evaluated. For transplantation, primordial germ cells of Astyanax altiparanae were transplanted into anaesthetised larvae (1 dph) of Prochilodus lineatus. Better results were obtained in the recirculation system for dechorionated embryos of P. lineatus for hatching (54.18%) and normal morphology (50.06%). The 2-phenoxyethanol anaesthetic with a dose of 29.3 mM resulted in shorter induction times, in addition to the recovery time between 5 and 10 min. By using this anaesthetic concentration at transplantation, GFP-positive cells were seen in two recipients, but the cells did not proliferate. This study established an effective incubation system for the development of the dechorionated embryo and determined an effective anaesthetic concentration for P. lineatus larvae. In addition, micromanipulation and transplantation of primordial germ cells in neotropical species were conducted for the first time.

Keywords

BiotechnologyDechorionated embryo and conservationMicromanipulation2-Phenoxyethanol
Type
Research Article
Information
Zygote , Volume 30 , Issue 6 , December 2022 , pp. 773 - 780
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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