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Kisspeptin stimulates sheep ovarian follicular development in vitro through homologous receptors

Published online by Cambridge University Press:  07 December 2023

B. Divya Sri ,
S. Harsha Lekha ,
K. Narendra Gopal Reddy ,
Deepa Pathipati ,
B. Rambabu Naik ,
P. Jagapathy Ramayya ,
K. Veera Bramhaiah ,
L.S.S. Varaprasad Reddy  and
A.V.N. Siva Kumar Open the ORCID record for A.V.N. Siva Kumar [Opens in a new window]
B. Divya Sri
Affiliation:
Embryo Biotechnology Laboratory, Department of Veterinary Physiology, College of Veterinary Science, S.V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
S. Harsha Lekha
Affiliation:
Embryo Biotechnology Laboratory, Department of Veterinary Physiology, College of Veterinary Science, S.V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
K. Narendra Gopal Reddy
Affiliation:
Embryo Biotechnology Laboratory, Department of Veterinary Physiology, College of Veterinary Science, S.V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
Deepa Pathipati
Affiliation:
Embryo Biotechnology Laboratory, Department of Veterinary Physiology, College of Veterinary Science, S.V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
B. Rambabu Naik
Affiliation:
Embryo Biotechnology Laboratory, Department of Veterinary Physiology, College of Veterinary Science, S.V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
P. Jagapathy Ramayya
Affiliation:
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, S. V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
K. Veera Bramhaiah
Affiliation:
Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, S. V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
L.S.S. Varaprasad Reddy
Affiliation:
Embryo Biotechnology Laboratory, Department of Veterinary Physiology, College of Veterinary Science, S.V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
A.V.N. Siva Kumar*
Affiliation:
Embryo Biotechnology Laboratory, Department of Veterinary Physiology, College of Veterinary Science, S.V. Veterinary University, Tirupati-517502, Andhra Pradesh, India
*
Corresponding author: A.V.N. Siva Kumar; Email: priyamshiva@yahoo.com
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Summary

The present study was conducted to elucidate (1) the influence of kisspeptin (KP) on the in vitro development of preantral follicles (PFs) and (2) evolution of KP receptor gene (KISS1R) expression during ovarian follicular development in sheep. Kisspeptin was supplemented (0–100 µg/ml) in the culture medium of PFs for 6 days. The cumulus–oocyte complexes (COCs) from cultured PFs were subsequently matured to metaphase II (MII) for an additional 24 h. The proportions of PFs exhibiting growth, antrum formation, average increase in diameter, and maturation of oocytes to MII stage were the indicators of follicular development in vitro. The expression of the kisspeptin receptor gene at each development stages of in vivo developed (preantral, early antral, antral, large antral and COCs from Graafian follicles) and in vitro cultured PFs supplemented with KP was assessed using a real-time polymerase chain reaction. The best development in all the parameters under study was elicited with 10 µg/ml of KP. Supplementation of KP (10 µg/ml) in a medium containing other growth factors (insulin-like growth factor-I) and hormones (growth hormone, thyroxine, follicle-stimulating hormone) resulted in better PF development. The KISS1R gene was expressed in follicular cells and oocytes at all the development stages of both in vivo developed and in vitro cultured follicles. Higher KISS1R gene expression was supported by culture medium containing KP along with other hormones and growth factors. Accordingly, it is suggested that one of the mechanisms through which KP and other growth factors and hormones influence the ovarian follicular development in mammals is through the upregulation of expression of the KP receptor gene.

Keywords

Culture of ovarian folliclesKisspeptinKisspeptin receptorSheep
Type
Research Article
Information
Zygote , Volume 32 , Issue 1 , February 2024 , pp. 49 - 57
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally.

References

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