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Microtubule-severing protein Fidgetin-like 1 promotes spindle organization during meiosis of mouse oocytes

Published online by Cambridge University Press:  23 September 2022

Hua-Feng Shou ,
Zhen Jin ,
Yan Yu ,
Yu-Cheng Lai ,
Qing Wu  and
Lei-Lei Gao Open the ORCID record for Lei-Lei Gao [Opens in a new window]
Hua-Feng Shou
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital, (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang310014, China
Zhen Jin
Affiliation:
Center for Reproductive Medicine, Department of Reproductive Endocrinology, Zhejiang Provincial People’s Hospital, (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang310014, China
Yan Yu
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital, (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang310014, China
Yu-Cheng Lai
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital, (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang310014, China
Qing Wu
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital, (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang310014, China
Lei-Lei Gao*
Affiliation:
Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People’s Hospital, (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang310014, China
*
Author for correspondence: Lei-Lei Gao. Department of Gynecology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang310014, China. E-mail: gaoleilei198802@126.com
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Summary

Microtubule-severing proteins (MTSPs) play important roles in mitosis and interphase. However, to the best of our knowledge, no previous studies have evaluated the role of MTSPs in female meiosis in mammals. It was found that FIGNL1, a member of MTSPs, was predominantly expressed in mouse oocytes and distributed at the spindle poles during meiosis in the present study. FIGNL1 was co-localized and interacted with γ-tubulin, an important component of the microtubule tissue centre (MTOC). Fignl1 knockdown by specific small interfering RNA caused spindle defects characterized by an abnormal length:width ratio and decreased microtubule density, which consequently led to aberrant chromosome arrangement, oocyte maturation and fertilization obstacles. In conclusion, the present results suggested that FIGNL1 may be an essential factor in oocyte maturation by influencing the meiosis process via the formation of spindles.

Keywords

MeiosisMicrotubuleMicrotubule-severing proteinsOocyteSpindle
Type
Research Article
Information
Zygote , Volume 30 , Issue 6 , December 2022 , pp. 872 - 881
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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