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Aging as Accelerated Accumulation of Somatic Variants: Whole-Genome Sequencing of Centenarian and Middle-Aged Monozygotic Twin Pairs
- Kai Ye, Marian Beekman, Eric-Wubbo Lameijer, Yanju Zhang, Matthijs H. Moed, Erik B. van den Akker, Joris Deelen, Jeanine J. Houwing-Duistermaat, Dennis Kremer, Seyed Yahya Anvar, Jeroen F. J. Laros, David Jones, Keiran Raine, Ben Blackburne, Shobha Potluri, Quan Long, Victor Guryev, Ruud van der Breggen, Rudi G. J. Westendorp, Peter A. C. ‘t Hoen, Johan den Dunnen, Gert Jan B. van Ommen, Gonneke Willemsen, Steven J. Pitts, David R. Cox, Zemin Ning, Dorret I. Boomsma, P. Eline Slagboom
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- Journal:
- Twin Research and Human Genetics / Volume 16 / Issue 6 / December 2013
- Published online by Cambridge University Press:
- 04 November 2013, pp. 1026-1032
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- Article
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It has been postulated that aging is the consequence of an accelerated accumulation of somatic DNA mutations and that subsequent errors in the primary structure of proteins ultimately reach levels sufficient to affect organismal functions. The technical limitations of detecting somatic changes and the lack of insight about the minimum level of erroneous proteins to cause an error catastrophe hampered any firm conclusions on these theories. In this study, we sequenced the whole genome of DNA in whole blood of two pairs of monozygotic (MZ) twins, 40 and 100 years old, by two independent next-generation sequencing (NGS) platforms (Illumina and Complete Genomics). Potentially discordant single-base substitutions supported by both platforms were validated extensively by Sanger, Roche 454, and Ion Torrent sequencing. We demonstrate that the genomes of the two twin pairs are germ-line identical between co-twins, and that the genomes of the 100-year-old MZ twins are discerned by eight confirmed somatic single-base substitutions, five of which are within introns. Putative somatic variation between the 40-year-old twins was not confirmed in the validation phase. We conclude from this systematic effort that by using two independent NGS platforms, somatic single nucleotide substitutions can be detected, and that a century of life did not result in a large number of detectable somatic mutations in blood. The low number of somatic variants observed by using two NGS platforms might provide a framework for detecting disease-related somatic variants in phenotypically discordant MZ twins.
Chapter 7 - Wind Energy
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- By Ryan Wiser, Zhenbin Yang, Maureen Hand, Olav Hohmeyer, David Infield, Peter H. Jensen, Vladimir Nikolaev, Mark O'Malley, Graham Sinden, Arthouros Zervos, Naïm Darghouth, Dennis Elliott, Garvin Heath, Ben Hoen, Hannele Holttinen, Jason Jonkman, Andrew Mills, Patrick Moriarty, Sara Pryor, Scott Schreck, Charles Smith, Christian Kjaer, Fatemeh Rahimzadeh
- Edited by Ottmar Edenhofer, Ramón Pichs-Madruga, Youba Sokona, Kristin Seyboth, Susanne Kadner, Timm Zwickel, Patrick Eickemeier, Gerrit Hansen, Steffen Schlömer, Christoph von Stechow, Patrick Matschoss
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- Book:
- Renewable Energy Sources and Climate Change Mitigation
- Published online:
- 05 December 2011
- Print publication:
- 21 November 2011, pp 535-608
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Summary
Executive Summary
Wind energy offers significant potential for near-term (2020) and long-term (2050) greenhouse gas (GHG) emissions reductions. A number of different wind energy technologies are available across a range of applications, but the primary use of wind energy of relevance to climate change mitigation is to generate electricity from larger, grid-connected wind turbines, deployed either on- or offshore. Focusing on these technologies, the wind power capacity installed by the end of 2009 was capable of meeting roughly 1.8% of worldwide electricity demand, and that contribution could grow to in excess of 20% by 2050 if ambitious efforts are made to reduce GHG emissions and to address the other impediments to increased wind energy deployment. Onshore wind energy is already being deployed at a rapid pace in many countries, and no insurmountable technical barriers exist that preclude increased levels of wind energy penetration into electricity supply systems. Moreover, though average wind speeds vary considerably by location, ample technical potential exists in most regions of the world to enable significant wind energy deployment. In some areas with good wind resources, the cost of wind energy is already competitive with current energy market prices, even without considering relative environmental impacts. Nonetheless, in most regions of the world, policy measures are still required to ensure rapid deployment. Continued advances in on- and offshore wind energy technology are expected, however, further reducing the cost of wind energy and improving wind energy's GHG emissions reduction potential.