Conservationists are raising concerns over high lion Panthera leo mortality and prey population declines in the area at the frontier between the Democratic Republic of Congo and Uganda. Confirming if threats to lions are severe or lion populations are disappearing requires extensive surveys on the ground because aerial detection of lions is inaccurate. Yet, ground surveys over large areas are unsafe or infeasible in the war-torn study area. We used aerial surveys of medium- to large-bodied ungulate prey to estimate lion abundance in two adjoining parks: Queen Elizabeth National Park, Uganda, and Parc National des Virunga, Democratic Republic of Congo. We validated two approaches to predict lion abundance using total counts of lions from Uganda. From this, we predict the two national parks together could have held 221 lions in 2004 and they have the potential to hold 905 lions if prey recover and lion-specific mortality is curbed. This makes the region a potential stronghold for the species in central Africa. However, a recent one third decline in lion numbers in the Ugandan Park and pervasive threats to the Congolese Park lead us to recommend immediate conservation intervention for lions and their prey. In Uganda, we recommend focused action to protect lions from poaching and retaliation, whereas in Congo, general enforcement of wildlife protection and a ground-based survey for lions are needed.

The idea of direct payments for biodiversity conservation in developing countries has generated much debate. Despite substantial experience with related economic instruments in high-income countries such approaches are rare in tropical developing countries, where conservation action is most urgently needed. We explore current experience with the application of direct payments in developing countries through an extensive review and subsequent analysis of the efforts of Conservation International. Our review identifies a broad spectrum of possible direct payment contracts. However, we focus on those involving international conservation interests. Firstly, we develop a framework for the design of direct payment applications, addressing four major aspects: contractual arrangements, definition of conservation services, performance payments, and monitoring and enforcement systems. Secondly, we discuss implementation issues, highlighting the need to consider social factors such as participatory processes, property rights, local institutions and contract legitimacy. Finally, we discuss important considerations for future payment schemes. These include the need for social responsibility, as well as rigorous assessments of effectiveness. We conclude that direct payments show potential as an innovative tool for engaging local communities or resource users in conservation and as a mechanism for channelling global investments in biodiversity conservation services to site-based initiatives.

Although hunting is still critical to the subsistence of many people throughout Amazonia, this practice may not be sustainable under current socio-economic conditions. Native societies are rapidly undergoing socio-economic changes that exacerbate the pressure on wildlife and habitats, indicating the urgent need to assess the impacts of subsistence hunting. In a 12-month study we assessed hunting patterns in four Shuar native communities in the Ecuadorian Amazon. Hunting patterns and impact of hunting activities were documented using interviews, direct observations, self-monitoring records, community landscape mapping and mammal surveys. Although Shuar harvest a wide-range of wildlife species, including insects, amphibians, reptiles, birds and mammals we only report information about mammals. A total of 3,181 individuals (c. 26,000 kg) of 21 mammal species were hunted during the 12 months. We used three algorithms for assessing the sustainability of hunting: the production, stock-recruitment and harvest models. Of the 21 mammal species hunted there were sufficient data to assess 15, 12 of which were hunted above maximum sustainable levels within the 243 km2 hunting catchment area. The immediate need to conserve wildlife populations is not obvious to Shuar hunters who still enjoy what they perceive to be an inexhaustible source of wild meat. In this context management of Shuar hunting practices to control harvest levels is complex. The assessment presented here is the first step of what needs to be a long-term wildlife management process.

The Critically Endangered Chinese crested tern Sterna bernsteini is a poorly known species. From June 2003 to August 2007 we therefore surveyed the Zhejiang and Shandong coasts of eastern China for breeding colonies and to document any threats. Our results indicated that (1) the colonies at Matzu and Jiushan are the only two in the species' potential breeding range, (2) the total population is no more than 50, (3) numbers in the two extant breeding colonies fluctuate annually, and (4) threats to the breeding populations include habitat degradation, egg poaching, disturbance, overfishing and typhoons. Egg poaching is the greatest threat to the Chinese crested tern population and other breeding seabird populations along the coast of China. We make recommendations for the conservation of this species.

The majority of Galliformes are ground dwelling, many live in forests, and c. 25% are on the IUCN Red List. The Djibouti francolin Francolinus ochropectus is a Critically Endangered galliform endemic to only two areas of relict Juniperus procera forest in Djibouti. This study assessed population status and habitat condition in the species' stronghold in the Forêt du Day during the post-breeding season. Line transect distance sampling was used to survey the francolin, recording visual encounters and calls. Canopy and understorey vegetation were sampled across the study area at 150-m intervals using 400-m2 quadrats. Interviews were conducted in all adjacent villages to obtain information about francolin sightings, forest use and capacity for community-based conservation. Distance was used to generate francolin population density estimates. A geographical information system and generalized linear modelling were used to determine predictors of francolin presence and juniper condition. The Distance model estimated francolin density to be 38–94 km-2. Within the Forêt du Day this is equivalent to a population of 285–705 individuals. The presence of juveniles in the samples suggests that the effective population size may be lower and therefore, although this is the first estimate of Djibouti francolin density using standard survey methodology, it should be interpreted cautiously. Juniper condition in the Forêt du Day is poor. The healthiest forest is 50% dead. Francolins are more abundant where tree cover is high. This cover now mostly consists of Buxus hildebrandtii, which appears to have mostly replaced the original juniper. In areas of high tree cover, grazing intensity is significantly negatively correlated with francolin presence. Anthropogenic influences on juniper health and francolin decline are mediated through the large number of cows grazing in the forest. We recommend an ecosystem approach to conservation of the forest, with additional species-specific protection measures for the francolin and juniper.

We conducted a questionnaire survey among 77 cattle posts and farms to investigate human-carnivore conflicts in northern Botswana, with a particular focus on Endangered African wild dog Lycaon pictus, persecuted throughout their shrinking range in sub-Saharan Africa for allegedly predating livestock. Predator attacks on livestock (n = 938 conflict reports) represent an economic concern for livestock owners, particularly alleged predation by black-backed jackal Canis mesomelas, which were blamed for 77% of all reported livestock losses. The presence of two known resident packs of wild dogs did not result in corresponding conflict reports with livestock owners, as wild dogs accounted for only 2% of reported predator attacks and largely subsisted on wild prey. Nevertheless, most of these wild dogs were killed in the months following this survey. Reported conflicts involving the two largest predator species (lion Panthera leo and spotted hyaena Crocuta crocuta) declined with increasing distance from protected areas. Leaving livestock unattended during the day seems to facilitate predation but kraaling livestock at night reduces predation. Compensation payments for livestock losses did not demonstrably change livestock owners’ willingness to coexist with predators. Our results corroborate studies from elsewhere that simple improvements in livestock husbandry practices would help mitigate human-carnivore conflicts.

In the 2003 Durban Vision the Malagasy government committed to tripling the amount of protected areas in Madagascar by 2009. This extensive expansion needs to involve an assessment of the potential impacts on the people who rely on forest resources for subsistence. Wildlife for human consumption (bushmeat) is one such resource that has received great attention on mainland Africa but has largely been ignored in Madagascar until recently. In terms of biomass, hunting in Madagascar appears to be on a lesser scale compared to areas of mainland Africa. However, because of the life-history characteristics associated with hunted primate and carnivore species in Madagascar even small-scale hunting is a major threat to long-term conservation. In this study I used semi-structured interviews to quantify annual rates of bushmeat harvest in 14 villages adjacent to the Makira Forest in north-eastern Madagascar. Interviews revealed that 23 mammal species were hunted for consumption, providing a new insight into the scale and frequency of bushmeat use. Harvest data and life-history information were sufficient to allow quantitative assessments of sustainability for four species of lemur (black and white ruffed lemur Varecia variegata, indri Indri indri, eastern bamboo lemur Hapalemur griseus and white-fronted brown lemur Eulemur albifrons) and a species of the carnivore family Eupleridae (fossa Cryptoprocta ferox). Model results suggest hunting of these species is probably unsustainable. This research presents clear evidence that hunting is a major conservation and livelihoods issue in Madagascar and needs to be considered in the planning stages of protected area development to address better the needs of local people.

China is the largest consumer of turtles in the world and international trade has been cited as the greatest threat to Asian turtles. Two main types of trade in live turtles occur in China: for food and traditional Chinese medicine, and for pets, including those for release by Buddhists. The food trade involves the largest quantities of turtles. In recent years, however, the international pet turtle trade has increased dramatically. Yuehe Pet Market in Guangzhou is the largest pet market in China. selling live chelonians and other animals. To understand the potential impacts of the pet trade on chelonians we conducted seven surveys in Yuehe Pet Market from August 2006 to March 2008. Over 39,000 individual chelonians of 61 species were recorded (19.1% of the global total of 319 species). Fifteen (24.6%) of these species are native to China and 46 (75.4%) are native to other countries. Two are designated as grade II key state-protected species in China. Thirty-eight (62.3%) are CITES listed species (four in CITES Appendix I, 26 in CITES II and eight in CITES III). Four are categorized on the IUCN Red List as Critically Endangered, 16 as Endangered and 19 as Vulnerable. Our surveys indicate that increasing demand and the illegal international pet trade could be having a severe impact on chelonian conservation, and we make recommendations for law enforcement and conservation.

Dispersal is fundamental to the persistence of wild populations. Historically, swift foxes Vulpes velox of the northern Great Plains of North America have been thought to be poor dispersers. Short-grass prairie is optimal habitat for swift foxes but can be fragmented in the northern Great Plains. We wanted to assess whether wild-born, juvenile swift foxes from two proximate but distinct reintroduced populations had potential to move from one population to the other. We found five animals exhibiting long bouts of dispersal, much further than averages previously reported. One female fox traversed the long distance between the two populations and survived for at least three breeding seasons in the wild. We believe our findings are significant for conservation because they show that swift foxes are not poor dispersers and that patches of short-grass prairie previously thought to be too isolated (> 25 km) for natural movement may be recolonized or be suitable for reintroductions of swift foxes.

The Vulnerable beira antelope Dorcatragus megalotis is endemic to the Horn of Africa and believed to occur in low numbers in Djibouti, in the southern mountain range of Aser-Jog. In the absence, however, of detailed information on the species, we censused the population and estimated its density, and collected data on the occurrence of potential competitors and predators. A total of 99 different individuals were sighted, with an estimated average density of 0.69 km-2 (range 0–1.25 km-2) over the 13 massifs comprising the mountain range. We did not locate the species in massifs located near villages, suggesting it is affected by human activities. One of the main threats to the beira may be competition for grazing with domestic goats. The creation of a wildlife refuge in Aser-Jog would increase the attractiveness of the area to tourists and encourage international organizations to support a conservation programme for the beira.

Worldwide many important tree resources, and the livelihoods of local community members who depend on them, are threatened. Effective management of tree resources requires, among other things, knowledge of which species are preferred locally and can therefore be successfully adopted into conservation plans, as well as an understanding of local attitudes towards tree conservation. We conducted a study in Nawaikoke Sub-county of eastern Uganda to address three questions: (1) Which woody species are considered most valuable to rural communities and how do priorities vary across different sectors of the communities? (2) What are local perceptions of the status of woody species and of the main threats to their conservation? (3) What attitudes do local people hold towards conservation and what challenges do conservation initiatives face? We carried out focus group discussions with different specialist groups, key informant interviews, and semi-structured interviews with 62 households. Our results illustrate that local communities value 26 woody species highly, 15 of which are indigenous. However, priorities vary greatly across different specialist user groups. According to respondents most of the prioritized species are threatened, with the greatest threats being charcoal burning, overexploitation and expanding agriculture. Community members are actively managing and/or interested in managing those tree resources with economic value but largely ignore the greater diversity of woody species, especially shrubs. Administrative, political and traditional leadership institutions do not currently assist in the conservation of woody species. We provide suggestions for a conservation action plan that involves multiple strategies.

A questionnaire survey and literature review revealed the extent of hunting of bats for bushmeat in the Old World tropics. High levels of offtake were reported throughout Asia, the Pacific islands and some Western Indian Ocean islands, where fruit bats of the genus Pteropus are eaten extensively. Most hunting in Africa was reported in western states and the largest fruit bat Eidolon helvum was preferred. Insectivorous bats are also eaten, particularly Tadarida in Asia. Hunting is both for local consumption and commercial, sometimes involving cross-border transactions. The high levels of hunting reported and the low reproductive rate of bats indicate there are likely to be severe negative effects on bat populations, and declines of several species are documented. Although there has been only one reported attempt to manage offtake, this indicates that it is possible and apparently successful. Furthermore, voluntary controls on hunting have halted declines in bat numbers. There have been several initiatives to reduce hunting pressure and conserve threatened bat species, mainly on islands that, when sustained, have been successful. More education projects and community-based conservation initiatives should be encouraged together with further attempts at sustainable harvesting in situations where disease risk has been evaluated.

There are three species of Neotropical cats whose northernmost distribution reaches the USA-Mexico border region: the jaguar Panthera onca, ocelot Leopardus pardalis and jaguarundi Herpailurus yagouaroundi. To ensure the long-term viability of Neotropical cat populations in this region it is imperative to identify current distribution and status in the northern limits of their range to conserve important populations effectively. The purpose of this study was to construct a blueprint of priority conservation areas for each species in the border region. This was done by (1) compiling reliable sightings for each species from the early 1900s to 2003, (2) conducting field surveys to ascertain species presence, and (3) conducting a geographical information system based habitat mapping workshop in which 29 scientists and conservationists provided information on the distribution and status of each species. Participants were asked to delineate and describe specific areas in the border region where historical and recent sightings of Neotropical cats have occurred, resulting in a compilation of 864 felid sightings of which 283 where reliable and had physical evidence. Twenty-one Cat Conservation Units and seven Cat Conservation Corridors were identified as areas in which to concentrate efforts for protecting felids. Only 8.9% of these Corridors and 1.1% of the Units currently have any protection. An additional 12 Corridors and 12 Units were identified as areas requiring further study.

Six marine turtle species are reported from the coastal waters of the Republic of the Congo. Among them, nesting by the Critically Endangered leatherback Dermochelys coriacea and Endangered olive ridley Lepidochelys olivacea marine turtles occurs annually from September to April on Congolese beaches. We developed a methodology to model the nesting season of marine turtles and apply it to the time series of nest counts for six nesting beaches monitored over 2–4 years. There is a peak of nesting activity in early January for leatherback turtles and early December for olive ridley turtles. We show a decline of olive ridley nests during this period whereas leatherback nesting increased, and propose that differential threats for these two species explain such a contrasted pattern.

Lac Alaotra is Madagascar's largest lake and a recognized wetland of international importance under the Ramsar Convention. It supports several Critically Endangered species, including the Alaotran gentle lemur Hapalemur alaotrensis. A principal threat facing the remaining Alaotran wetland is anthropogenic burning of the vegetation during the dry season; a practice now officially banned. A number of reasons have been given to explain this but to date no attempt has been made to investigate the principal motivations for the burning. We present the results of semi-structured interviews (n = 27) and questionnaires (n = 122) in a lakeside town. Seventy-eight percent of interviewees stated wetland burning was performed only or mainly to gather an introduced fish, the Asian snakehead Channa maculata, which buries into the underlying substrate during the dry season. Sixty-eight percent of questionnaire respondents provided a similar explanation. These data provide valuable insights into the reasons for the ongoing burning and should inform the management actions that are required to protect this globally important wetland.

The Vulnerable Antillean manatee Trichechus manatus manatus (Mammalia, Sirenia) is threatened by hunting, entanglement in fishing nets and habitat degradation but most knowledge of the species comes from coastal and estuarine populations. We therefore studied an Antillean manatee population located >1,100 km from the Caribbean coast, in the Orinoco River of Colombia, during 2001–2005. To verify the occurrence of the manatee and to investigate its habitat use throughout the annual hydrological cycle, we carried out surveys for manatees and manatee feeding signs over c. 40 km of the Orinoco River, between El Burro and Bachaco. We made 870 sightings in 1,003 hours of observation, and found 69 feeding areas in 776 hours of surveys. From 82 interviews in a total of 28 locations in Venezuela and Colombia we compiled 90 reports of manatees injured or killed in the Orinoco during 1980–2004. Both habitat use by the manatee and anthropogenic impacts on the species vary with the annual hydrological cycle. Reproduction appears to occur during the low water period in restricted areas that retain deep water. Improved knowledge of the dynamics of manatee migration and regulation of human activities, especially those related to use of nets and boat traffic, are required for conservation of the Antillean manatee in this freshwater habitat.

The estimated number of olive ridley marine turtles Lepidochelys olivacea nesting annually in 2002–2007 in French Guiana was 1,716–3,257, the highest ever recorded in the country and similar to nesting numbers recorded in neighbouring Suriname c. 40 years ago, where the species has now severely declined. A shift of nesting females from Suriname to French Guiana beaches and improvement of nationwide marine turtle monitoring appear to be the most plausible explanations for the current high level of nesting recorded in French Guiana. The species' nesting status in French Guiana therefore appears less critical than previously documented but ongoing threats suggest the need to reinforce regional conservation efforts in the West Atlantic.

The flare horned markhor Capra falconeri occurs in northern Pakistan, Afghanistan, India, Uzbekistan and Tajikistan. Most of the species’ range is along volatile international borders and limited information is available, especially for the population of the Pir Panjal or Kashmir markhor C. f. falconeri in India. From October 2004 to April 2005 we therefore conducted the first range-wide survey of the species in India since independence. The markhor's range has shrunk from c. 300 km2 in the late 1940s to c. 120 km2 in 2004–2005. Our surveys and interviews with key local informants indicate that 350–375 markhor may yet exist in the region. All the populations are small (usually < 50) and fragmented. International conflicts, developmental projects, the needs of an increasing human population and poaching, along with lack of awareness, are the primary threats to the species. The largest population in India, in Kajinag, may have potential for long-term survival if immediate conservation measures can be implemented.

The impact of alien American mink on the native fauna of oceanic islands has been demonstrated in a number of locations. In the sub-Antarctic Cape Horn Biosphere Reserve of southern Chile the species is currently expanding in an area where the native fauna evolved in the absence of terrestrial predators. To evaluate any emerging problems we therefore investigated seasonal variation in prey use by mink on Navarino Island within the Reserve. We identified undigested remains in 414 scats collected from the shores of 27 ponds over January–November 2006. Diet consisted mainly of mammals and birds. Mammals, including both native and exotic rodents, were the predominant prey in all seasons but birds were of equal importance during the summer (when birds breed and their abundance and diversity increases on the island). Exotic rodents were the only identifiable mammalian prey item during winter. Native wetland birds constituted a substantial proportion of mink diet, and greater than that reported in other areas. Many birds breeding on Navarino Island are ground-nesting, a strategy that evolved in the absence of native mammalian predators. Considering the international importance of this region, our results emphasize the need for an assessment of the impact of mink predation on the populations of native prey.

Waterholes are a limited resource vital to the conservation of biodiversity in arid ecosystems. Given the rarity of natural waterholes in deserts and their presumed importance to Vulnerable Nubian ibex Capra nubiana, we examined the influence of landscape characteristics and anthropogenic factors on ibex presence at waterholes. Our results suggest that anthropogenic factors play a larger role in waterhole use than landscape characteristics. Ibex used waterholes regardless of maximum waterhole diameter, maximum water depth or width of the valley in which the waterhole was located. However, ibex were significantly more likely to use waterholes that were far from human dwellings and that had not been visited recently by feral donkeys. Waterhole and ibex conservation will require working with local communities to protect, and ensure sustainable use of, this vital resource.