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Association between AUTS2 haplotypes and alcohol dependence in a Japanese population

Published online by Cambridge University Press:  14 January 2016

Shin Narita ,
Kenta Nagahori ,
Daisuke Nishizawa ,
Eiji Yoshihara ,
Atsuko Kawai ,
Kazutaka Ikeda  and
Kazuhiko Iwahashi
Shin Narita
Affiliation:
Laboratory of Physiology (Project of Neurophysiology), Course of Environmental Health Science, Graduate School of Environmental Health, Azabu University, Sagamihara-shi, Kanagawa, Japan
Kenta Nagahori
Affiliation:
Department of Anatomy, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan
Daisuke Nishizawa
Affiliation:
Addictive Substance Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan
Eiji Yoshihara
Affiliation:
Laboratory of Physiology (Project of Neurophysiology), Course of Environmental Health Science, Graduate School of Environmental Health, Azabu University, Sagamihara-shi, Kanagawa, Japan
Atsuko Kawai
Affiliation:
Koutokukai Total Health Clinic, Yamagata, Japan
Kazutaka Ikeda
Affiliation:
Addictive Substance Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan
Kazuhiko Iwahashi*
Affiliation:
Laboratory of Physiology (Project of Neurophysiology), Course of Environmental Health Science, Graduate School of Environmental Health, Azabu University, Sagamihara-shi, Kanagawa, Japan Addictive Substance Project, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo, Japan Health Administration Center, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan
*
Kazuhiko Iwahashi, Laboratory of Physiology (Project of Neurophysiology), the Graduate School of Environmental Health Sciences, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan. Tel: +81 42 769 1930; Fax: +81 42 769 1930; E-mail: iwahashi@azabu-u.ac.jp
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Abstract

Objective

Recent genome-wide analysis has indicated that the autism susceptibility candidate 2 (AUTS2) gene is involved in the regulation of alcohol consumption. We hypothesised that AUTS2 might be associated with the development of alcohol dependence. Therefore, in this exploratory study, we compared the genotype and allele frequencies of the polymorphisms rs6943555 and rs9886351 in the AUTS2 gene between patients with alcohol dependence and healthy control subjects living in a Japanese provincial prefecture. We also examined whether or not the haplotypes consisting of these polymorphisms are related to alcohol dependence.

Methods

The subjects of this study consisted of 64 patients with alcohol dependence and 75 unrelated healthy people. The AUTS2 genotypes were determined by the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method.

Results

No significant differences in the genotype and allele frequencies of the polymorphisms AUTS2 rs6943555 and rs9886351 were found between alcohol dependence and control subjects. On the other hand, the frequencies of the AUTS2 haplotypes were significantly different between them, and the rs6943555 and rs9886351 A-A haplotype was associated with alcohol dependence (p=0.0187).

Conclusion

This suggests that the rs6943555 and rs9886351 A-A haplotype might affect the vulnerability to alcohol dependence pathogenesis. Further studies are needed to confirm the reproducibility of the results of this study with increased numbers of subjects.

Keywords

alcohol dependenceautism susceptibility candidate 2gene polymorphismhaplotype
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 28 , Issue 4 , August 2016 , pp. 214 - 220
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Copyright
© Scandinavian College of Neuropsychopharmacology 2016 

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