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Steroid production and LH receptor concentrations of ovarian follicles and corpora lutea and associated rates of ova wastage in ewes given high and low levels of food intake before and after mating

Published online by Cambridge University Press:  02 September 2010

J. A. Abecia ,
S. M. Rhind ,
T. A. Bramley  and
S. R. McMillen
J. A. Abecia
Affiliation:
Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB9 2QJ
S. M. Rhind
Affiliation:
Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB9 2QJ
T. A. Bramley
Affiliation:
Department of Obstetrics and Gynaecology, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW
S. R. McMillen
Affiliation:
Department of Obstetrics and Gynaecology, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW
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Abstract

Two groups of eives were fed to provide 1·5 × (high, H; no. = 13) or 0·5 × (low, L; no. = 12) energy requirements for maintenance of live weight from 12 days before a synchronized mating in November until slaughter at 14 days after mating and the effects on embryo survival and associated patterns of gonadotropin secretion, and ovarian follicle and corpus luteum function were investigated. Proportionately, there were more pregnant ewes in the H group than the L group (0·62 v. 0·08; %2 = 7·67; P < 0·01) at day 14 of pregnancy but there were no differences in mean LH concentrations, LH pulse frequencies or amplitudes, either before mating and ovulation (follicular phase) or at day 10 after mating (luteal phase). The mean size (mm) of the three largest follicles (H: 5·69; L: 5·65; s.e.d. = 0·21), the proportion of these follicles that were oestrogenic (secreting > 500 pg oestradiol per h; H: 0·29; L: 0·28; y) = 0·01; P > 0·05) and secretion (pg/h) in vitro of oestradiol (H, 294; L, 386; s.e.d. = 146) (pg/h) and testosterone (H: 636; L: 508; s.e.d. = 293) by these follicles were similar for both treatments. There were no treatment differences in LH receptor concentrations (pg hormone bound per mg protein) in granulosa (H: 69·02; L: 67·76; s.e.d. = 0·20) and thecal (H: 46-88; L: 50·82; s.e.d. = 0·19) tissues. However, there was a higher concentration of receptors in the thecal tissue of oestrogenic follicles ofL than H ewes (167 v. 62; s.e.d. = 18; P < 0·05). Mean weights (g) of corpora lutea (H: 0·72; L: 0·59; s.e.d. = 0·003; P = 0·09), progesterone secretion (ng/mg per h) in vitro by luteal tissue (H: 1·75; L: 1·78; s.e.d. = 0·30) and LH receptor concentrations in corpora lutea (H: 58·16; L: 54·27; s.e.d. = 25·14) were similar for both treatments. It is concluded that the reduction in embryo survival associated with a reduced level of food intake was not attributable to a reduction in LH secretion, inadequacies in follicle growth and development or in the capacity of the corpora lutea to synthesize and release progesterone.

Keywords

ewesfood intakehormonesova wastagereceptors
Type
Research Article
Information
Animal Science , Volume 61 , Issue 1 , August 1995 , pp. 57 - 62
Copyright
Copyright © British Society of Animal Science 1995

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References

Agricultural Research Council. 1980. The nutrient requirements of ruminant livestock. Commonwealth Agricultural Bureaux, Slough.Google Scholar
Ashworth, C. J. and Bazer, F. W. 1989. Interrelationships of proteins secreted by the ovine conceptus and endometrium during the periattachment period. Animal Reproduction Science 20:117–130.Google Scholar
Ashworth, C. J., Wilmut, I. and Sales, D. I. 1989. Effect of the stage of the oestrus cycle and the frequency of sample collection on the composition of ovine uterine fluids. Animal Reproduction Science 21:213–221.Google Scholar
Baird, D. T. 1992. Luteotrophic control of the corpus luteum. Animal Reproduction Science 28:95–102.CrossRefGoogle Scholar
Baird, D. T., Swanston, I. A. and McNeilly, A. S. 1981. Relationship between LH, FSH and prolactin concentration and the secretion of androgens and estrogens by the preovulatory follicle in the ewe. Biology of Reproduction 24:1013–1025.Google Scholar
Bramley, T. A., Stirling, D., Swanston, I. A., Menzies, G. S. and Baird, D. T. 1987. Specific binding sites for LH, chorionic gonadotrophin, low density lipoprotein, prolactin and FSH in homogenates of human corpus luteum. 1. Validation of methods, journal of Endocrinology 113:305–315.Google Scholar
Edey, T. N. 1966. Nutritional stress and preimplantation embryonic mortality in Merino sheep. Journal of Agricultural Science, Cambridge 67:287–302.Google Scholar
English, J., Poulton, A. L., Arendt, J. and Symons, A. M. 1986. A comparison of the efficiency of melatonin treatments in advancing oestrus in ewes. Journal of Reproduction and Fertility 77:321–327.Google Scholar
Garverick, H. A. and Smith, M. F. 1986. Mechanisms associated with subnormal luteal function. Journal of Animal Science 62: suppl. 2, pp. 92–105.Google Scholar
Gore-Langton, R. A. and Armstrong, D. T. 1988. Follicular steroidogenesis and its control. In The physiology of reproduction (ed. Knobil, E. and Neill, J.), pp. 331–385. Raven Press, New York.Google Scholar
Hansel, W. and Fortune, J. 1978. The applications of ovulation control. In Control of-ovulation (ed. Crighton, D. B., Hayes, N. B., Foxcroft, G. R. and Lamming, G. E.), pp. 237–263. Butterworth, London.Google Scholar
Haresign, W. 1981. The influence of nutrition on reproduction in the ewe. 1. Effects on ovulation rate, follicle development and luteinizing hormone. Animal Production 32:197–203.Google Scholar
Hunter, M. G., Southee, J. A., McLeod, B. J. and Haresign, W. 1986. Progesterone pretreatment has a direct effect on GnRH-induced preovulatory follicles to determine their ability to develop into normal corpora lutea in anoestrous ewes. Journal of Reproduction and Fertility 76:349–363.Google Scholar
Lawes Agricultural Trust. 1992. Genstat 5 reference manual. Oxford University Press.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275.Google Scholar
McNeilly, A. S., Jonassen, J. A. and Fraser, H. M. 1986. Suppression of follicular development after chronic LHRH immunoneutralization in the ewe. journal of Reproduction and Fertility 76:481–490.Google Scholar
McNeilly, J. R., McNeilly, A. S., Walton, J. S. and Cunningham, F. J. 1976. Development and application of a heterologous radioimmunoassay for ovine follicle-stimulating hormone. Journal of Endocrinology 70:69–79.CrossRefGoogle ScholarPubMed
Parr, R. A., Cumming, I. A. and Clarke, I. J. 1982. Effects of maternal nutrition and plasma progesterone concentrations on survival and growth of the sheep embryo in early gestation. Journal of Agricultural Science, Cambridge 98:39–46Google Scholar
Parr, R. A., Davis, I. F., Fairclough, R. J. and Miles, M. A. 1987. Overfeeding during early pregnancy reduces peripheral progesterone concentration and pregnancy rate in sheep. Journal of Reproduction and Fertility 80:317–320.Google Scholar
Rhind, S. M., Leslie, I. D., Gunn, R. G. and Doney, J. M., 1985. Plasma FSH, LH, prolactin and progesterone profiles of Cheviot ewes with different levels of intake before and after mating, and associated effects on reproductive performance. Animal Reproduction Science 8:301–313.CrossRefGoogle Scholar
Rhind, S. M., McKelvey, W. A. C., McMillen, S., Gunn, R. G. and Elston, D. A. 1989a. Effect of restricted food intake, before and/or after mating, on the reproductive performance of Greyface ewes. Animal Production 48:149–155.Google Scholar
Rhind, S. M., McMillen, S., Wetherill, G. Z., McKelvey, W. A. C. and Gunn, R. G. 1989b. Effects of low levels of food intake before and/or after mating on gonadotropin and progesterone profiles in Greyface ewes. Animal Production 49:267–273.Google Scholar
Russel, A. J. F., Doney, J. M. and Gunn, R. G. 1969. Subjective assessment of body fat in live sheep, journal of Agricultural Science, Cambridge 72:451–454.Google Scholar
Webb, R., Gauld, I. K. and Driancourt, M. A. 1989. Morphological and functional characterization of large antral follicles in three breeds of sheep with different ovulation rates. Journal of Reproduction and Fertility 87:243–255.Google Scholar