Caractérisation des particules virales planctoniques dans un lac du Massif Central français : aspects méthodologiques et premiers résultats

T. Sime-Ngando; J.-P. Mignot; C. Amblard; G. Bourdier; C. Desvilettes; C. Quiblier-Lloberas

doi:10.1051/limn/1996025

Abstract

Dans cette note, nous décrivons une méthode simple, permettant l'ultracentrifugation de particules virales planctoniques et leur observation en microscopie électronique à transmission. Une description de différents morphotypes de particules assimilables à des virus est faite à partir d'échantillons prélevés dans la couche euphotique d'un lac eutrophe (Aydat), au cours des périodes printanière et estivale. Ces particules présentent chacune une capside icosaédrique régulière à 6 faces et une queue, dont les dimensions sont similaires à celles des phages décrits en milieu marin. La diversité des formes et le nombre de virions (10 fois supérieur à celui des bactéries), permettent d'émettre l'hypothèse selon laquelle les virus interviennent de façon significative dans le contrôle des abondances des communautés bactériennes en milieu lacustre.

Crossref Citations

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Caractérisation des particules virales planctoniques dans un lac du Massif Central français : aspects méthodologiques et premiers résultats

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Caractérisation des particules virales planctoniques dans un lac du Massif Central français : aspects méthodologiques et premiers résultats

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