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The effect of flow turbulence on growth, nutrient uptake and stable carbon and nitrogen isotope signatures in Chara fibrosa

Published online by Cambridge University Press:  18 September 2012

Champika Ellawala ,
Takashi Asaeda  and
Kiyoshi Kawamura
Champika Ellawala*
Affiliation:
Department of Civil and Environmental Engineering, Faculty of Engineering, University of Ruhuna, Hapugala, Galle, Sri Lanka Institute for Environmental Science and Technology, Saitama University, 255 Shimo-okubo, Sakura, Saitama 338-8570, Japan
Takashi Asaeda
Affiliation:
Institute for Environmental Science and Technology, Saitama University, 255 Shimo-okubo, Sakura, Saitama 338-8570, Japan
Kiyoshi Kawamura
Affiliation:
Institute for Environmental Science and Technology, Saitama University, 255 Shimo-okubo, Sakura, Saitama 338-8570, Japan
*
*Corresponding author: ellawala@eng.ruh.ac.lk
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Abstract

Exposure to water movement has been observed to alter various processes in the plants including growth, hormone concentration and nutrient uptake. In the current study, Chara fibrosa was exposed to three different turbulence conditions and compared with a control. Turbulence was generated in the laboratory by using a vertically oscillating grid setup. Exposure to turbulence caused a reduction in shoot length and nutrient uptake in C. fibrosa. Variation of stable isotope composition was measured as a surrogate variable that is able to integrate variations of many physiological processes. It was initially hypothesized that the reduction of diffusion boundary layer around the plant will increase isotopic discrimination against 13C and 15N, when exposed to increasing turbulence. Although the results generally agree with the hypothesis, a trend of increment was observed in δ13C in the plants exposed to turbulent velocities from 0.46 to 1.93 cm s−1, against the hypothesis. Mechanical stress induced reduction of carbon uptake and lipid peroxidation due to the development of oxidative stress may be the reasons behind the above-mentioned trend. The study exhibits that the net effect of physical and physiochemical changes of the plants was displayed in δ13C signatures and it is important to consider physical conditions of the local environment, in using stable isotope signatures for ecosystem studies.

Keywords

Turbulenceisotopestresscarbonnitrogen
Type
Research Article
Information
Annales de Limnologie - International Journal of Limnology , Volume 48 , Issue 3 , 2012 , pp. 349 - 354
Copyright
© EDP Sciences, 2012

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References

Amor, F.M.D. and Cuadra-Crespo, P., 2011. Alleviation of salinity stress in broccoli using foliar urea or methyl-jasmonate: analysis of growth, gas exchange and isotope composition. Plant Growth Regul., 63, 55–62.CrossRefGoogle Scholar
APHA (Ed.) 1998. Standard Methods for the Examination of Water and Wastewater. APHA, AWWA, and WEF, Washington.
Chehab, W., Eich, E. and Braam, J., 2009. Thigmomorphogenesis: a complex plant response to mechano-stimulation. J. Exp. Bot., 60, 43–56.CrossRefGoogle ScholarPubMed
De Silva, I.P.D. and Fernando, H.J.S., 1994. Oscillating grids as a source of nearly isotropic turbulence. Phys. Fluids, 6, 2455–2464.CrossRefGoogle Scholar
Ellawala, C., Asaeda, T. and Kawamura, K., 2011a. Influence of flow turbulence on Chara fibrosa: growth, stress, and tissue carbon content. J. Freshwater Ecol., 26, 507–515.Google Scholar
Ellawala, K.C., Asaeda, T. and Kawamura, K., 2011b. The effect of flow turbulence on plant growth and several growth regulators in Egeria densa Planchon. Flora, 206, 1085–1091.CrossRefGoogle Scholar
Farquhar, G.D., Ehleringer, R. and Hubic, K.T., 1989. Carbon isotope discrimination and photosynthesis. Annu. Rev. Plant Physiol. Plant Mol. Biol., 40, 503–537.CrossRefGoogle Scholar
Finlay, J.C., Power, M.E. and Cabana, G., 1999. Effects of water velocity on algal carbon isotope ratios: implications for river food web studies. Limnol. Ocean., 44, 1198–1203.CrossRefGoogle Scholar
Gomes, P.I.A. and Asaeda, T., 2009. Phycoremediation of chromium (vi) by Nitella and impact of calcium encrustation. J. Hazard. Mater., 166, 1332–1338.CrossRefGoogle ScholarPubMed
Hussain, M.I. and Reigosa, M.J., 2011. A chlorophyll fluorescence analysis of photosynthetic efficiency, quantum yield and photon energy dissipation in psii antennae of Lactuca sativa L. Leaves exposed to cinnamic acid. Plant Physiol. Biochem., 49, 1290–1298.CrossRefGoogle ScholarPubMed
Jardine, K., Karl, T., Lerdau, M., Harley, P., Guenther, A. and Mak, J.E., 2009. Carbon isotope analysis of acetaldehyde emitted from leaves following mechanical stress and anoxia. Plant Biol., 11, 591–597.CrossRefGoogle ScholarPubMed
Ke, X. and Li, W., 2006. Hormonal correlates of seedling growth of two Vallisneria species grown at different current velocities. Hydrobiologia, 556, 243–249.CrossRefGoogle Scholar
Li, Y., Zhao, H., Duan, B., Korpelainen, H. and Li, C., 2011. Effect of drought and ABA on growth, photosynthesis and antioxidant system of Cotinus coggygria seedlings under two different light conditions. Environ. Exp. Bot., 71, 107–113.CrossRefGoogle Scholar
Madsen, J.D., Chambers, P.A., James, W.F., Koch, E.W. and Westlake, D.F., 2001. The interaction between water movement, sediment dynamics and submersed macrophytes. Hydrobiologia, 444, 71–84.CrossRefGoogle Scholar
Madsen, T. and Sondergaard, M., 1983. The effects of current velocity on the photosynthesis of Callitriche stagnalis scop. Aquat. Bot., 15, 187–193.CrossRefGoogle Scholar
Madsen, T.V., Enevoldsen, H.O. and Jørgensen, T.B., 1993. Effects of water velocity on photosynthesis and dark respiration in submerged stream macrophytes. Plant Cell Environ., 16, 317–322.CrossRefGoogle Scholar
Nishihara, G.N. and Ackerman, J.D., 2006. The effect of hydrodynamics on the mass transfer of dissolved inorganic carbon to the freshwater macrophyte Vallisneria americana. Limnol. Ocean., 51, 2734–2745.CrossRefGoogle Scholar
O'Brien, K., Meyer, D., Waite, A., Ivey, G. and Hamilton, D., 2004. Disaggregation of Microcystis aeruginosa colonies under turbulent mixing: laboratory experiments in a grid-stirred tank. Hydrobiologia, 519, 143–152.CrossRefGoogle Scholar
Parida, A.K. and Jha, B., 2010. Antioxidative defense potential to salinity in the euhalophyte Salicornia brachiata. J. Plant Growth Regul., 29, 137–148.CrossRefGoogle Scholar
Rasmussen, J.B. and Trudeau, V., 2007. Influence of velocity and chlorophyll standing stock on periphyton δ13C and δ15N in the ste. Marguerite river system, Quebec. Can. J. Fish. Aquat. Sci., 64, 1370–1381.CrossRefGoogle Scholar
Robinson, D., Handley, L.L., Scrimgeour, G.M., Gordon, D.C., Forster, D.B. and Ellis, R.P., 2000. Using stable isotope natural abundences (δ15N and δ13C) to integrate stress responses of wild barley (Hordeum spontaneum C. Koch.) genotypes. J. Exp. Bot., 55, 41–50.Google Scholar
Sand-Jensen, K. and Pedersen, O., 1999. Velocity gradients and turbulence around macrophyte stands in streams. Freshwater Biol., 42, 315–328.CrossRefGoogle Scholar
Sanford, L.P., 1997. Turbulent mixing in experimental ecosystem studies. Marine Ecol. Prog. Series, 161, 265–293.CrossRefGoogle Scholar
Szmeja, J. and Galka, A., 2008. Phenotypic responses to water flow and wave exposure in aquatic plants. Acta Soc. Bot. Pol., 77, 59–65.Google Scholar
Trudeau, V.R. and Rasmussen, J.B., 2003. The effect of water velocity on stable carbon and nitrogen isotope signatures of periphyton. Limonol. Ocean., 48, 2194–2199.CrossRefGoogle Scholar
Westlake, D.F., 1967. Some effects of low velocity currents on the metabolism of aquatic macrophytes. J. Exp. Bot., 18, 187–207.CrossRefGoogle Scholar
Wuest, A. and Lorke, A., 2003. Small scale hydrodynamics in lakes. Ann. Rev. Fluid Mech., 35, 373–412.CrossRefGoogle Scholar
Zeng, Q.F., Kong, F.X., Zhang, E.L., Tan, X. and Wu, X.D., 2008. Seasonality of stable carbon and nitrogen isotopes within the pelagic food web of Taihu lake. Ann. Limnol. ‐ Int. J. Lim., 44, 1–6.CrossRefGoogle Scholar
Zhang, H., Ye, Y.K., Wang, S.H., Luo, J.P., Tang, J. and Ma, D.F., 2009. Hydrogen sulfide counteracts chlorophyll loss in sweet potato seedling leaves and alleviates oxidative damage against osmotic stress. Plant Growth Regul., 58, 243–250.CrossRefGoogle Scholar