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Dominant symbiotic bacteria associated with wild medfly populations reveal a bacteriocin-like killing phenotype: a ‘cold-case’ study

Published online by Cambridge University Press:  09 December 2019

Silvia Ciolfi Open the ORCID record for Silvia Ciolfi [Opens in a new window]  and
Laura Marri Open the ORCID record for Laura Marri [Opens in a new window]
Silvia Ciolfi
Affiliation:
Department of Life Sciences, via A. Moro 2, University of Siena, Siena-53100, Italy
Laura Marri*
Affiliation:
Department of Life Sciences, via A. Moro 2, University of Siena, Siena-53100, Italy
*
Author for correspondence: Laura Marri, Email: laura.marri@unisi.it
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Abstract

The gut of the agricultural pest Ceratitis capitata hosts a varied community of bacteria, mainly Enterobacteriaceae, that were implicated in several processes that increase the fitness of the insect. In this study, we investigated the antagonistic activity in vitro of Klebsiella oxytoca strains isolated in the 1990s from the alimentary tract of wild medflies collected from different varieties of fruit trees at diverse localities. Assays were carried out against reference strains (representative of Gram-negative and -positive bacterial species) of the American Type Culture Collection (ATCC). Eight Klebsiella, out of 11, expressed a killing activity against Escherichia coli ATCC 23739, and Enterobacter cloacae ATCC 13047; among the eight strains, at least one showed activity against Salmonella typhimurium ATCC 23853. Genomic DNA derived from all Klebsiella strains was then subjected to PCR amplification using specific primer pairs designed from each of the four bacteriocin (KlebB, C, D, CCL) sequences found so far in Klebsiella. KlebD primer pairs were the only to produce a single product for all strains expressing the killing phenotype in vitro. One of the amplicons was cloned and sequenced; the DNA sequence shows 93% identity with a plasmid-carried colicin-D gene of a strain of Klebsiella michiganensis, and 86% identity with the sequence encoding for the klebicin D activity protein in K. oxytoca. Our work provides the first evidence that dominant symbiotic bacteria associated with wild medfly populations express a killing phenotype that may mediate inter and intraspecies competition among bacterial populations in the insect gut in vivo.

Keywords

BacteriocinsCeratitiscompetitionenterobacteriacaeKlebsiellasymbiosistephritidae
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 110 , Issue 4 , August 2020 , pp. 457 - 462
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Copyright
Copyright © Cambridge University Press 2019

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