Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-04-30T18:20:33.723Z Has data issue: false hasContentIssue false
  • English
  • Français

Egg laying suppression in Drosophila melanogaster (Diptera: Drosophilidae) and Dacus (Bactrocera) oleae (Diptera: Tephritidae) by phloroglucinol, a peroxidase inhibitor

Published online by Cambridge University Press:  10 July 2009

K.E. Keramaris ,
L.H. Margaritis ,
E.N. Zografou  and
G.J. Tsiropoulos
K.E. Keramaris
Affiliation:
Department of Biochemistry, Cell-Molecular Biology and Genetics, Athens University, Greece
L.H. Margaritis
Affiliation:
Department of Biochemistry, Cell-Molecular Biology and Genetics, Athens University, Greece
E.N. Zografou
Affiliation:
Institute of Biology, NCSR ‘Demokritos’, Athens, Greece
G.J. Tsiropoulos*
Affiliation:
Institute of Biology, NCSR ‘Demokritos’, Athens, Greece
*
* Correspondence: G.J. Tsiropoulos, Institute of Biology, ‘Demokritos’, National Research Center, Aghia Paraskevi 153 10 Attiki, Greece.
Get access Rights & Permissions [Opens in a new window]

Abstract

Eggshell peroxidase (ESP) is responsible for the hardening process in several Diptera, including Drosophila melanogaster Meigen and Bactrocera oleae Gmelin. Its action can be inhibited by phloroglucinol, a natural phenolic substance, during the formation of the egg-shell. We used phloroglucinol, in the diet of adults of D. melanogaster and B. oleae, at concentrations of 1 mM, 10 mM, 25 mM, 50 mM, 100 mM and 400 mM to study its effect on egg laying. In both insects, 1 mM had no effect. At concentrations above 10 mM, egg laying of D. melanogaster was gradually affected leading to the deposition of empty shells and chorion-less eggs, while in B. oleae, it was gradually suppressed and only amorphous masses were laid. The effect of phloroglucinol at the tested concentrations was reversible. It is concluded that phloroglucinol added at appropriate, non-lethal concentrations, affected egg-laying of D. melanogaster and B. oleae in different ways, related to differences in the ovipositor diameter relative to egg size. In B. oleae, it is relatively narrow, causing breakage of the phloroglucinol-induced non-elastic egg-shell, since covalent crosslinking of the chorion proteins is prevented. In D. melanogaster, chorion-less eggs and separate chorions are laid, due to egg dechorionation. A possible field application of these results is discussed.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 86 , Issue 4 , August 1996 , pp. 369 - 374
Copyright
Copyright © Cambridge University Press 1996

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ananthakrishanan, T.N., Senrayan, R., Annadurai, R.S. & Murogesan, S. (1990) Antibiotic effects of resorcinol gallic acid and phloroglucinol on Heliothis armigera Huebner, Insecta Noctuidae. Proceedings of the Indian Academy of Sciences 99, 39–52.Google Scholar
Brune, A. & Schink, B. (1990) Pyrogallol-to-phloroglucinol conversion and other hydroxyl-transfer reactions catalyzed by cell extracts of Pelobacter acibigallici. Journal of Bacteriology 172, 1070–1076.Google Scholar
Economopoulos, A.P. & Tzanakakis, M.E. (1967) Egg yolk and olive juice as supplements to the yeast hydrolyzate-sucrose diet for adults of Dacus oleae. Life Science 6, 2409–2416.Google Scholar
Haddock, J.D. & Ferry, J.G. (1989) Purification and properties of phloroglucinol reductase from Eubacterium oxidoreducens G-41*. Journal of Biological Chemistry 264, 4423.Google Scholar
Keramaris, K.E., Stravopodis, D. & Margaritis, L.H. (1991) Structural protein that plays an enzymatic role in the eggshell of Drosophila melanogaster. Cell Biology International Reports, 15, 151–159.Google Scholar
Krumholz, L.R. & Bryand, M.P. (1986) Characterization of the pyrogallol-phloroglucinol isomerase of Eubacterium oxidoreducens. Journal of Bacteriology 170, 2472.Google Scholar
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680–685.Google Scholar
Margaritis, L.H. (1985a) Comparative study of the eggshell of the fruit flies Dacus oleae and Ceratitis capitata. Canadian Journal of Zoology 63, 2194–206.Google Scholar
Margaritis, L.H. (1985b) Structure and physiology of the eggshell, pp. 153–230. in Gilbert, L.I. & Kerkut, G.A. (Eds) Comprehensive insect biochemistry, physiology and pharmacology, Vol. 1. Oxford and New York, Pergamon Press.Google Scholar
Margaritis, L.H. (1985c) The egg-shell of Drosophila melanogaster III. Covalent crosslinking of the chorion protein involves endogenous hydrogen peroxide. Tissue and Cell 17, 553–559.CrossRefGoogle ScholarPubMed
Margaritis, L.H. (1986) The eggshell of Drosophila melanogaster II. New staging characteristics and the five structural analysis of choriogenesis. Canadian journal of Zoology 64, 2152–2175.CrossRefGoogle Scholar
Mindrinos, M.N., Petri, W.H., Galanopoulos, V.K., Lombard, M.F. & Margaritis, L.H. (1980) Crosslinking of the Drosophila chorion involves a peroxidase. Wilhelm Roux's Archives 189, 187–196Google Scholar
Mouzaki, D.G., Zarani, F.E. & Margaritis, L.H. (1991) Structure and morphogenesis of the eggshell and micropylar apparatus of the olive fly Dacus oleae (Diptera: Tephritidae). Journal of Morphology 208, 1–14.Google Scholar
Patel, T.R., Hameed, N. & Martin, A.M. (1990) Initial steps of phloroglucinol metabolism in Penicillium simplicissimum. Archives of Microbiology 153, 438–443.Google Scholar
Petri, W.H., Mindrinos, M.N., Lombard, M.F., & Margaritis, L.H. (1971) In vitro development of the Drosophila chorion in a chemically defined organ culture medium. Wilhelm Roux's Archives 186, 351–362.Google Scholar
Pruidze, G.N., Grigorashvili, G.Z., Chachua, L.S.H. & Tokhadze, M.V. (1976) Purification and properties of peroxidase EC-1.11.1.7. from tea leaves. Biokhimiya 41, 1819–1828.Google Scholar
Toiguchi, S., Hayashi, K., Adachi, Y., Motoki, M. & Haruguchi, K. (1989) Purification and characterization of soybean oxidase. Journal of the Japanese Society of Food Science and Technology 36, 597–602.Google Scholar
Tsiropoulos, J.G. (1985) Dietary administration of antivitamins affected the survival and reproduction of D. oleae. Zeitschrift für Angewandte Entomologie 100, 35–39.Google Scholar