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Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)

Published online by Cambridge University Press:  28 March 2016

M. Nenadić ,
M. Soković ,
J. Glamočlija ,
A. Ćirić ,
V. Perić-Mataruga ,
V. Tešević ,
L. Vujisić ,
M. Todosijević ,
N. Vesović  and
S. Ćurčić
M. Nenadić
Affiliation:
Institute of Zoology, University of Belgrade – Faculty of Biology, Studentski Trg 16, 11000 Belgrade, Serbia
M. Soković
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
J. Glamočlija
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
A. Ćirić
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
V. Perić-Mataruga
Affiliation:
Institute for Biological Research ‘Siniša Stanković’, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia
V. Tešević
Affiliation:
University of Belgrade – Faculty of Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia
L. Vujisić
Affiliation:
University of Belgrade – Faculty of Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia
M. Todosijević
Affiliation:
University of Belgrade – Faculty of Chemistry, Studentski Trg 12-16, 11000 Belgrade, Serbia
N. Vesović
Affiliation:
Institute of Zoology, University of Belgrade – Faculty of Biology, Studentski Trg 16, 11000 Belgrade, Serbia
S. Ćurčić*
Affiliation:
Institute of Zoology, University of Belgrade – Faculty of Biology, Studentski Trg 16, 11000 Belgrade, Serbia
*
*Author for correspondence Tel: +381 11 2187266 Fax: +381 11 2638500 E-mail: srecko@bio.bg.ac.rs
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Abstract

The antimicrobial activity of the pygidial gland secretion released by adult individuals of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828), applying microdilution method with the aim to detect minimal inhibitory concentration, minimal bactericidal concentration and minimal fungicidal concentration, has been investigated. In addition, morphology of the pygidial glands is observed. We have tested 16 laboratory and clinical strains of human pathogens – eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples have showed antimicrobial properties against all strains of treated bacteria and fungi. Micrococcus flavus proved to be more resistant compared with other bacterial strains. More significant antimicrobial properties of the secretion are observed against Escherichia coli, which proved to be the most sensitive bacteria. Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and Penicillium verrucosum var. cyclopium the most sensitive micromycetes. Commercial antibiotics Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied as positive controls, showed higher antibacterial properties for all bacterial and fungal strains, except for P. ochrochloron, which proved to be more resistant on Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart from the role in ecological aspects, the antimicrobial properties of the tested secretion possibly might have medical significance in the future.

Keywords

Laemostenus (Pristonychus) punctatuspygidial gland secretionantibacterial and antifungal activityhuman pathogensmicrodilutionmorphology
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 106 , Issue 4 , August 2016 , pp. 474 - 480
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Copyright
Copyright © Cambridge University Press 2016 

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