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Extent of digestion affects the success of amplifying human DNA from blood meals of Anopheles gambiae (Diptera: Culicidae)

Published online by Cambridge University Press:  09 March 2007

W.R. Mukabana ,
W. Takken ,
P. Seda ,
G.F. Killeen ,
W.A. Hawley  and
B.G.J. Knols
W.R. Mukabana*
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), PO Box 30772, Nairobi, Kenya Laboratory of Entomology, Wageningen University and Research Center, PO Box 8031, 6700 EH Wageningen, The Netherlands Department of Zoology, University of Nairobi, PO Box 30197, Nairobi, Kenya
W. Takken
Affiliation:
Laboratory of Entomology, Wageningen University and Research Center, PO Box 8031, 6700 EH Wageningen, The Netherlands
P. Seda
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), PO Box 30772, Nairobi, Kenya
G.F. Killeen
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), PO Box 30772, Nairobi, Kenya Department of Tropical Medicine, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
W.A. Hawley
Affiliation:
Division of Parasitic Diseases, Centers for Disease Control and Prevention (CDC), MF22, GA 30333, USA
B.G.J. Knols
Affiliation:
International Centre of Insect Physiology and Ecology (ICIPE), PO Box 30772, Nairobi, Kenya
*
*Fax: +254 385 22190 E-mail: rmukabana@mbita.mincom.net
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Abstract

The success of distinguishing blood meal sources of Anopheles gambiae Giles through deoxyribonucleic acid (DNA) profiling was investigated by polymerase chain reaction (PCR) amplification at the TC-11 and VWA human short tandem repeats (STR) loci. Blood meal size and locus had no significant effect on the success of amplifying human DNA from blood meals digested for 0, 8, 16, 24 and 32 h (P = 0.85 and 0.26 respectively). However, logistic regression found a significant negative relationship between time since ingestion and the success probability of obtaining positive PCR products among meals digested for between 8 and 32 h (P = 0.001). Approximately 80% of fresh blood meals were successfully profiled. After 8 h, the proportion of blood meals that could be successfully profiled decreased slowly with time after ingestion, dropping to below 50% after approximately 15 h. There was no significant difference in the success of amplifying human DNA from blood meals of mosquitoes killed at time 0 and 8 h after ingestion (P = 0.272).

Type
Research Article
Information
Bulletin of Entomological Research , Volume 92 , Issue 3 , June 2002 , pp. 233 - 239
Copyright
Copyright © Cambridge University Press 2002

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