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DIGESTIVE ENZYMES OF SIXTH-INSTAR LARVAE OF THE PALE WESTERN CUTWORM, AGROTIS ORTHOGONIA (LEPIDOPTERA: NOCTUIDAE)

Published online by Cambridge University Press:  31 May 2012

M. R. Khan  and
R. Kasting
M. R. Khan
Affiliation:
Research Station, Canada Department of Agriculture, Lethbridge, Alberta
R. Kasting
Affiliation:
Research Station, Canada Department of Agriculture, Lethbridge, Alberta
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Abstract

Results of a qualitative survey of some digestive enzymes of sixth-instar larvae of the pale western cutworm are reported. An extract prepared from the mesenteron (midgut) and its contents showed activity for the enzymes, proteinase, aminopeptidase, dipeptidase, amylase, α-glucosidasc, α-galactosidase, and lipase. There was also evidence for a β-glucosidase activity in the brei prepared from the mesenteron. A brei of salivary glands showed activities for α-glucosidase and amylase, as well as a strong proteinase. These enzymes were not found in brei prepared from the stomodaeum (foregut) or proctodaeum (hindgut). There was no evidence for polygalacturonase, celiulase, β-galactosidase, or carboxypeptidase in any part of the digestive system.Values of pH for extracts from the different parts of the digestive system were: proctodaeum, 7.2; mesenteron, 8.2; stomodaeum, 7.8; and salivary glands, 7.8.

Type
Articles
Information
The Canadian Entomologist , Volume 101 , Issue 5 , May 1969 , pp. 494 - 499
Copyright
Copyright © Entomological Society of Canada 1969

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