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Molecular evidence for sympatric taxa within Pemphigus betae (Hemiptera: Aphididae: Eriosomatinae)

  • R. G. Foottit (a1), K. Floate (a2) and E. Maw (a1)
Abstract
Abstract

Mitochondrial DNA cytochrome c oxidase subunit 1 (DNA barcode) and nuclear microsatellite flanking region sequences were used to analyse populations of putative “sugarbeet root aphid”, Pemphigus betae Doane, from sites in Alberta, Canada. Three sympatric genotypes were revealed, identified as P. betae, P. populivenae Fitch, and an undetermined third species. All three genotypes formed morphologically indistinguishable galls on the same set of cottonwood (Populus L., Salicaceae) host species, often on the same tree. Gall morphology is frequently used to identify Pemphigus species. Our results indicate that this practice may be unreliable for these three taxa at least.

Résumé

Les séquences de la sous-unité 1 de la cytochrome c oxydase de l'ADN mitochondrial (code à barres de l'ADN) et de la région flanquante des microsatellites nucléaires nous ont servi à analyser les populations de l'espèce putative du « puceron de la betterave à sucre », Pemphigus betae Doane, de sites de l'Alberta (Canada). Il existe trois génotypes sympatriques, identifiés comme P. betae, P. populivenae Fitch et une troisième espèce non identifiée. Les trois génotypes forment tous des galles qui sont impossibles à distinguer sur le même ensemble d'espèces-hôtes de peupliers (Populus L., Salicaceae), souvent sur le même arbre. La morphologie des galles sert souvent à identifier les espèces de Pemphigus; nos résultats indiquent que cette procédure n’est pas fiable au moins pour ces trois taxons.

[Traduction par la Rédaction]

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1 Corresponding author (e-mail: Robert.Foottit@agr.gc.ca).
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J.R. deWaard , N.V. Ivanova , M. Hajibabaei , and P.D.N. Hebert 2008. Assembling DNA barcodes: analytical protocols. InEnvironmental genomics: methods in molecular biology. Vol. 410. Edited byC.C. Martin . Humana Press, Totowa, New Jersey. pp. 275293.

K.D. Floate 2004. Extent and patterns of hybridization among the three species of Populus that constitute the riparian forest of southern Alberta, Canada. Canadian Journal of Botany, 82: 253264. doi:10.1139/b03-135.

K.D. Floate , and T.G. Whitham 1993. The “hybrid bridge” hypothesis: host shifting via plant hybrid swarms. The American Naturalist 141: 651662. PMID:19426003 doi:10.1086/285497.

K.D. Floate , G.D. Martinsen , and T.G. Whitham 1997. Cottonwood hybrid zones as centers of abundance for gall aphids in western North America: importance of relative habitat size. Journal of Animal Ecology, 66: 179188. doi:10.2307/6020.

R.G. Foottit , H.E.L. Maw , C.D von Dohlen , and P.D.N. Hebert 2008. Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes. Molecular Ecology Resources, 8: 11891201. doi:10.1111/j.1755-0998.2008.02297.x.

A.A. Grigarick , and W.H. Lange 1962. Host relationships of the sugar-beet root aphid in California. Journal of Economic Entomology, 55: 760764.

P.D.N. Hebert , A. Cywinska , S.L. Ball , and J.R. deWaard 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270: 313322.

W.D. Hutchison , and C.D. Campbell 1994. Economic impact of sugarbeet root aphid (Homoptera: Aphididae) on sugarbeet yield and quality in southern Minnesota. Journal of Economic Entomology, 87: 465475.

M. Kimura 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16: 11120. PMID:7441778 doi:10.1007/BF01731581.

A.C. Maxson 1916. Some unpublished notes on Pemphigus betae Doane. Journal of Economic Entomology, 9: 500504.

N.A. Moran , and T.G. Whitham 1988. Evolutionary reduction of complex life cycles: loss of host-alternation in Pemphigus (Homoptera: Aphididae). Evolution, 42: 717728. doi:10.2307/2408863.

D Posada , and K.A. Crandall 1998. Modeltest: testing the model of DNA substitution. Bioinformatics, 14: 817818. PMID:9918953 doi:10.1093/bioinformatics/14.9.817.

S. Ratnasingham , and P.D.N. Hebert 2007. BOLD: the Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7: 355364. PMID:18784790 doi:10.1111/j.1471-8286.2007.01678.x.

T.G. Whitham 1989. Plant hybrid zones as sinks for pests. Science (Washington, D.C.), 244: 14901493.

T.G. Whitham , G.D. Martinsen , K.D. Floate , H.S. Dungey , B.M. Potts , and P. Keim 1999. Plant hybrid zones affect biodiversity: tools for a genetic-based understanding of community structure. Ecology, 80: 416428. doi:10.1890/0012-9658(1999)080[0416:PHZABT]2.0.CO;2.

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The Canadian Entomologist
  • ISSN: 0008-347X
  • EISSN: 1918-3240
  • URL: /core/journals/canadian-entomologist
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