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NUCLEOPOLYHEDROVIRUS PATHOLOGY IN SPRUCE BUDWORM LARVAE

Published online by Cambridge University Press:  31 May 2012

J.W. Barrett ,
T.R. Ladd ,
M.J. Primavera ,
A. Retnakaran ,
S.S. Sohi  and
S.R. Palli
J.W. Barrett*
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada P6A 5M7
T.R. Ladd
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada P6A 5M7
M.J. Primavera
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada P6A 5M7
A. Retnakaran
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada P6A 5M7
S.S. Sohi
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada P6A 5M7
S.R. Palli
Affiliation:
Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, Ontario, Canada P6A 5M7
*
1 Author to whom all corresponding should be sent at the following address: Viral Immunology and Pathology, Robarts Research Institute, 1400 Western Road, London, Ontario, Canada N6G 2V4 (E-mail: jbarrett@rri.on.ca).
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Abstract

Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae) multiple nucleopolyhedrovirus (CfMNPV) expressing green fluorescent protein was used to study aspects of nucleopolyhedrovirus infection in the spruce budworm. The temporal and spatial distribution of fluorescence indicated that the virus infected the midgut, entered the tracheal system, and traveled to the epidermis, fat body, and muscles. In contrast to Autographa californica (Speyer) (Lepidoptera: Noctuidae) multiple nucleopolyhedrovirus (AcMNPV) infection, hemocytes from infected C. fumiferana did not exhibit fluorescence until after CfMNPV had passed from the midgut into the tracheae. Therefore the role of hemocytes may be limited during CfMNPV infection. Also the fluorescence pattern spread from the tracheolar cells to tracheal epithelial cells throughout the tracheal system. Our results indicate that the temporal and spatial events involved in CfMNPV infection of C. fumiferana larvae are consistent with those observed in other lepidopteran hosts infected with AcMNPV Minor deviations between these two systems may be attributed to differences in virulence, infection rate, and possibly host range of the virus.

Résumé

Le nucléopolyhédrovirus multiple de la Tordeuse des bourgeons de l’épinette (Choristoneura fumiferana) (CfMNPV) à protéine fluorescente verte (GFP) a été utilisé pour étudier certains aspects de ce type d’infection chez la tordeuse. La répartition temporelle et spatiale de la fluorescence indique que le virus infecte l’intestin moyen, pénètre dans le système trachéen et se répand dans l’épiderme, le corps gras et les muscles. Contrairement à ce qui se produit dans une infection du nucléopolyhédrovirus multiple de l’Autographa californica (Speyer) (Lepidoptera : Noctuidae) (AcMNPV), les hémocytes des tordeuses infectées ne deviennent pas fluorescents avant que le virus CfMNPV ne soit passé de l’intestin moyen au système trachéen. Le rôle des hémocytes est probablement limité dans ce type d’infection. De plus, la fluorescence se répand des cellules trachéolaires aux cellules épithéliales dans tout le système trachéen. Nos résultats indiquent que les étapes du déroulement temporel et spatial de l’infection CfMNPV chez les larves de la tordeuse sont semblables à celles observées chez d’autres lépidoptères infectés du virus AcMNPV. Les différences mineures entre ces deux systèmes peuvent être le résultat de différences dans le degré de virulence, dans le taux d’infection et probablement aussi dans l’éventail des hôtes du virus.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 132 , Issue 5 , October 2000 , pp. 581 - 590
Copyright
Copyright © Entomological Society of Canada 2000

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