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Use of expressed sequence tag microsatellite markers for population genetic research of Helicoverpa armigera (Lepidoptera: Noctuidae) from India

Published online by Cambridge University Press:  14 July 2015

Thiruvengadam Venkatesan ,
Vaddi Sridhar ,
Yan R. Tomason ,
Sushil Kumar Jalali ,
Gajanan T. Behere ,
Rajagopalan M. Shanthi ,
Ravinder Kumar ,
Venkata Gopinath Vajja ,
Padma Nimmakayala  and
Umesh K. Reddy
Thiruvengadam Venkatesan*
Affiliation:
ICAR-National Bureau of Agricultural Insect Resources, Bangalore 560 024, India
Vaddi Sridhar
Affiliation:
ICAR-Indian Institute of Horticultural Research, Bangalore 560 089, India
Yan R. Tomason
Affiliation:
Gus R. Douglass Institute and Department of Biology, West Virginia State University, West Virginia 25112-1000, United States of America
Sushil Kumar Jalali
Affiliation:
ICAR-National Bureau of Agricultural Insect Resources, Bangalore 560 024, India
Gajanan T. Behere
Affiliation:
Indian Council of Agricultural Research-Research Complex-North East Region, Umiam 793 103, Meghalaya, India
Rajagopalan M. Shanthi
Affiliation:
Indian Council of Agricultural Research-Sugarcane Breeding Institute, Coimbatore 641 007, India
Ravinder Kumar
Affiliation:
Indian Council of Agricultural Research-Sugarcane Breeding Institute, Coimbatore 641 007, India
Venkata Gopinath Vajja
Affiliation:
Gus R. Douglass Institute and Department of Biology, West Virginia State University, West Virginia 25112-1000, United States of America
Padma Nimmakayala
Affiliation:
Gus R. Douglass Institute and Department of Biology, West Virginia State University, West Virginia 25112-1000, United States of America
Umesh K. Reddy
Affiliation:
Gus R. Douglass Institute and Department of Biology, West Virginia State University, West Virginia 25112-1000, United States of America
*
2Corresponding author (e-mail: tvenkat12@gmail.com).
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Abstract

Cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is a serious pest of several crops throughout the world, representing millions of United States of America dollars worth of damage. This pest can adapt to various cropping systems in a wide geographical range and has high migratory potential. It features high fecundity and can develop resistance to almost all insecticides used for its management. Several investigations to develop microsatellite markers for H. armigera have not been successful because of the paucity of microsatellites in the lepidopteran genome. As well, collections of H. armigera from cotton fields of southern and western India were not yet studied for molecular genetic diversity. The current study aimed to screen publicly available expressed sequence tag resources for simple sequence repeats and assess their potential as DNA markers for assessment of gene flow between collections of southern and western India. We identified 30 polymorphic microsatellites for potential use in diversity analysis of H. armigera collections. Genetic diversity analysis revealed that the collections were widely diverse with population differentiation index (Fst) of 0.17. Furthermore, gene flow analysis revealed a mean frequency of private alleles of 11% within the collections. The microsatellite resources we developed could be widely used for molecular diversity or population genetic research involving this important pest of cotton and food crops.

Type
Biodiversity & Evolution
Information
The Canadian Entomologist , Volume 148 , Supplement 2 , April 2016 , pp. 187 - 199
Copyright
© Entomological Society of Canada 2015 

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Footnotes

Subject editor: Amanda Roe

1

Contributed equally to the first author.

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