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Tomato yellow leaf curl disease in Guangdong is caused by Tomato leaf curl Taiwan virus

Published online by Cambridge University Press:  02 August 2007

He Zi-Fu ,
Yu Hao ,
Mao Ming-Jie ,
Luo Fang-Fang ,
Lin Yi-Han  and
Wang Sui-Tao
He Zi-Fu*
Affiliation:
Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Yu Hao
Affiliation:
Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Mao Ming-Jie
Affiliation:
Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China
Luo Fang-Fang
Affiliation:
Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Lin Yi-Han
Affiliation:
Baisha Vegetable Institute, Shantou 515800, China
Wang Sui-Tao
Affiliation:
Baisha Vegetable Institute, Shantou 515800, China
*
*Corresponding author. E-mail: hezf@gdppri.com
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Abstract

A yellow leaf curl disease with chlorotic and yellowish leaves, upward leaf curling and stunting symptoms was observed on tomato in Shantou city of Guangdong province. A virus isolate BS was obtained from a diseased tomato plant. The complete DNA-A sequence of the virus isolate BS was determined to be 2740 nucleotides long, with all the characteristic features of begomovirus genome organization. BS DNA-A encoded six potential open reading frames (ORFs), with two (AV1 and AV2) in virus sense and four (AC1, AC2, AC3 and AC4) in complementary sense, and contained an intergenic region of 269 nucleotides. The results of BLAST searches showed that BS DNA-A had higher sequence identity with reported begomoviruses in Asia than with those in America and Africa. Further sequence comparisons indicated that BS was most closely related to the isolate of Tomato leaf curl Taiwan virus (ToLCTWV-[Taiwan]) with a sequence identity of 97.7%. Nucleotide sequence identities of AV1, AV2, AC1, AC2, AC3, AC4 and intergenic region (IR) between BS and ToLCTWV-[Taiwan] were 98.6, 98.0, 98.0, 97.5, 96.3, 98.6 and 96.6%, respectively, while that of the six ORF-encoded proteins between BS and ToLCTWV-[Taiwan] were 97.7, 99.1, 97.5, 95.6, 91.8 and 99.0%, respectively. Phylogenetic analysis based on the DNA-A sequences has also indicated that BS is most closely related to ToLCTWV-[Taiwan], forming a branch with ToLCTWV-[Taiwan], Tomato leaf curl Guangdong virus and Tomato yellow leaf curl Guangdong virus. The above results demonstrate that BS is an isolate of ToLCTWV.

Keywords

tomato yellow leaf curl diseaseTomato leaf curl Taiwan virusBS isolatebegomovirus
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 2 , August 2007 , pp. 127 - 131
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(1): 119–123

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