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Effect of food components and processing parameterson DNA degradation in food

Published online by Cambridge University Press:  15 June 2005

Torsten Bauer ,
Walter P. Hammes ,
Norbert U. Haase  and
Christian Hertel
Torsten Bauer
Affiliation:
Institute of Food Technology, University of Hohenheim, 70593 Stuttgart, Germany
Walter P. Hammes
Affiliation:
Institute of Food Technology, University of Hohenheim, 70593 Stuttgart, Germany
Norbert U. Haase
Affiliation:
Federal Centre for Cereal, Potato and Lipid Research, 32765 Detmold, Germany
Christian Hertel
Affiliation:
Institute of Food Technology, University of Hohenheim, 70593 Stuttgart, Germany

Abstract

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The effect of food components on degradation of DNA by DNase I (EC 3.1.21.1) was monitored by electrotransformation of Escherichia coli, making it possible to determine the number of plasmid molecules capable of giving rise to transformed cells. The transformation frequency increased linearly with the plasmid number within the range of 2 × 106 to 2 × 1010. DNA degradation was reduced by one order of magnitude in the presence of 0.05% (w.v–1) maltol or 1 mM putrescine. Complete inhibition of degradation was observed with ≥0.2% (w.v–1) maltol, ≥0.01% (w.v–1) octyl gallate or ≥0.5 mM of spermine. To monitor degradation of plant DNA during food processing, a real-time PCR system was established. The ratio of copy numbers of a potato gbss DNA fragment of 325 bp and a nested 96 bp fragment was determined. The latter served as internal reference for normalization. The system made it possible to exclude process-dependent changes of DNA concentration in the food matrix. Processing of genetically modified potatoes to dried potato sticks, crisps or flakes was studied and drying steps were shown to exert the strongest effect on DNA degradation, resulting in a drop of the ratio from 0.73 to 0.16.

Keywords

food processingfood componentspotatoDNA stabilityreal-time PCR
Type
Research Article
Information
Environmental Biosafety Research , Volume 3 , Issue 4 , October 2004 , pp. 215 - 223
Copyright
© ISBR, EDP Sciences, 2004

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