Human activities have fragmented amphibian habitats and affected amphibian diversity and distribution, but the ecology is poorly known. A four-year study assessed the diversity and distribution of amphibians in undisturbed and disturbed sites of the Kudremukh National Park (KNP), India. Iron-ore mining and associated activities in the KNP have induced habitat fragmentation. The disturbed sites had ranges of habitat variables clearly distinguishable from undisturbed sites. Thirty-six species of anurans and six species of caecilians have been recorded in the KNP and the total amphibian species richness represents 20% of the whole Indian amphibian fauna. Among these, 20 species were distributed in both disturbed and undisturbed sites, while 22 were found only in undisturbed sites indicating they may be threatened by further habitat fragmentation. Species diversity and richness formed two distinct groups clearly associated with disturbed and undisturbed habitats, respectively.